| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg | |||
| Other Sizes |
| Targets |
L012 sodium does not have a specific biological target; instead, it acts as a chemiluminescent probe that reacts with reactive oxygen species (ROS), particularly superoxide anions (O₂•⁻) and peroxynitrite. The probe is oxidized by ROS, producing light that can be measured quantitatively. It is used to detect NADPH oxidase (Nox)-derived superoxide and to identify Nox inhibitors. L012 sodium is highly sensitive and produces significantly higher luminescence yields than other CL probes.
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|---|---|
| ln Vitro |
L012 Sodium salt is a chemical analogue of Luminol that, when compared to MCLA and Lucigenin, other CL highlights, greatly enhances luminescence production.
In vitro, L012 sodium is used to detect superoxide production in cell-free systems and cultured cells. The probe is added to samples containing NADPH oxidase or other ROS-generating systems, and the resulting chemiluminescence is measured using a luminometer. L012 sodium greatly enhances luminescence production compared to MCLA and lucigenin. It is used to screen for Nox inhibitors and to study the role of ROS in various biological processes. The probe's high sensitivity allows for the detection of low levels of superoxide. |
| ln Vivo |
It is possible to track the growth and control of biosensors by utilizing the robust ROS/RNS-dependent luminescence signals that are mediated in vivo by the sodium salt L-012 [2], which is widely distributed in mice.
In vivo, L012 sodium is used to track ROS/RNS-dependent luminescence signals in mice. The probe is widely distributed in mice after administration, allowing for real-time imaging of ROS production in living animals. It is used to study the role of NADPH oxidase in inflammation, cardiovascular disease, and neurodegeneration. The robust luminescence signals mediated by L012 sodium enable the monitoring of biosensor growth and control in vivo. |
| Enzyme Assay |
The non-cellular assay for L012 sodium involves measuring chemiluminescence in response to superoxide generated by a cell-free system. Xanthine/xanthine oxidase or other superoxide-generating systems are incubated with L012 sodium, and the luminescence is measured using a luminometer. The intensity of the chemiluminescence is proportional to the amount of superoxide produced. The probe is used to screen for Nox inhibitors by measuring the reduction in luminescence in the presence of test compounds.
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| Cell Assay |
The cellular assay for L012 sodium involves treating cultured cells (e.g., macrophages, neutrophils, or endothelial cells) with the probe and measuring chemiluminescence in response to cellular ROS production. Cells are stimulated with phorbol myristate acetate (PMA) or other activators of NADPH oxidase, and L012 sodium is added to the culture medium. The resulting chemiluminescence is measured using a luminometer or a plate reader equipped with luminescence detection. The probe is used to assess the effects of Nox inhibitors or genetic manipulations on cellular ROS production.
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| Animal Protocol |
In the in vivo imaging study, L012 sodium is administered to mice by intraperitoneal or intravenous injection. The probe is widely distributed in tissues, and ROS-dependent luminescence is measured using an in vivo imaging system (IVIS). Mice may be subjected to inflammatory stimuli (e.g., LPS injection) to induce ROS production. The luminescence signal is quantified and compared between treated and control groups.
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| ADME/Pharmacokinetics |
L012 sodium has a molecular weight of 310.67 and a molecular formula of C₁₃H₈ClN₄NaO₂. The compound is soluble in DMSO (approximately 50 mg/mL) and in water (approximately 3.33 mg/mL). It should be stored as a powder at -20°C for up to 3 years or at 4°C for up to 2 years. In solvent, it should be stored at -80°C for 6 months or at -20°C for 1 month. The compound should be stored in a sealed and protected environment (e.g., under nitrogen) to avoid exposure to moisture.
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| Toxicity/Toxicokinetics |
L012 sodium is generally considered to have low toxicity at the concentrations used for chemiluminescence detection. However, as a reactive compound, it should be handled with appropriate laboratory safety precautions. The compound is not approved for human use and is strictly a research reagent.
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| References |
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| Additional Infomation |
L012 sodium is a highly sensitive chemiluminescent probe for the detection of superoxide and other reactive oxygen species. It is a derivative of luminol and is commonly used for the detection of NADPH oxidase-derived superoxide. Its high sensitivity and luminescence yield make it a valuable tool for studying oxidative stress in inflammation, cardiovascular disease, and neurodegenerative disorders. L012 sodium is not a therapeutic agent and is strictly a research tool.
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| Molecular Formula |
C13H8CLN4NAO2
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|---|---|
| Molecular Weight |
310.67
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| Exact Mass |
310.023
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| CAS # |
143556-24-5
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| PubChem CID |
78243715
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| Appearance |
White to yellow solid powder
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
21
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| Complexity |
430
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
IGEUYSJHQQCEFP-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C13H9ClN4O2.Na/c14-11-8-7(12(19)17-18-13(8)20)9(15)10(16-11)6-4-2-1-3-5-6;/h1-5H,15H2,(H,17,19)(H,18,20);/q;+1/p-1
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| Chemical Name |
sodium;8-amino-5-chloro-1-oxo-7-phenyl-2H-pyrido[3,4-d]pyridazin-4-olate
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| Synonyms |
L-012 sodium; L012 sodium; L012 sodium salt
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~160.94 mM)
H2O : ~3.33 mg/mL (~10.72 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (4.02 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (4.02 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.2188 mL | 16.0942 mL | 32.1885 mL | |
| 5 mM | 0.6438 mL | 3.2188 mL | 6.4377 mL | |
| 10 mM | 0.3219 mL | 1.6094 mL | 3.2188 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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