| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 100mg |
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| 250mg | |||
| Other Sizes |
| Targets |
L-NMMA acetate targets all three isoforms of nitric oxide synthase (NOS): neuronal NOS (nNOS, NOS1), endothelial NOS (eNOS, NOS3), and inducible NOS (iNOS, NOS2). It acts as a competitive inhibitor, meaning it competes with the natural substrate, L-arginine, for binding to the enzyme's active site. By inhibiting NOS, L-NMMA acetate prevents the conversion of L-arginine to L-citrulline and the subsequent release of nitric oxide (NO). The compound exhibits different inhibitory potencies for the three isoforms, with Ki values of approximately 0.18 µM for nNOS (rat), 0.4 µM for eNOS (human), and 6 µM for iNOS (mouse).
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| ln Vitro |
From 100 μM onward, L-NMMA generated concentration-dependent suppression (2Hz) of induced relaxation; the average maximal inhibition at 1 mM was about 35%. When L-arginine (L-Arg) was previously incubated with D-arginine, the inhibitory action of L-NMMA remained unaltered. The relaxation brought on by isoproterenol is unaffected by L-NMMA [2]. L-NMMA infusion decreased arteriolar diameter and increased arteriolar tone in a dose-dependent manner. L-NMMA functions quite instantly. When it comes to the endothelium-dependent vasodilator ACh, but not the endothelium-independent NP, L-NMMA reduces the vasodilatory response. L-NMMA dramatically increases the dose-related vasoconstriction that NE produces [3].
In vitro, L-NMMA acetate is a potent and effective inhibitor of NOS activity. Its activity is assessed by measuring the inhibition of NO production in various cell-free and cell-based systems. The compound's inhibitory potency is characterized by its Ki values for the different NOS isoforms. It is a standard reference inhibitor used to validate NOS activity assays and to study the role of NO in cellular signaling. Its effectiveness in inhibiting all three isoforms makes it a broad-spectrum tool for NO research. |
| ln Vivo |
In vivo, L-NMMA acetate is used to study the physiological and pathophysiological roles of NO. By systemically inhibiting NOS, it can be used to assess the contribution of NO to blood pressure regulation, vascular tone, and various disease models. It is a valuable tool for investigating the role of the NO pathway in conditions such as hypertension, inflammation, and septic shock. Detailed in vivo data from specific animal models are not extensively provided in the referenced sources.
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| Enzyme Assay |
Non-cell-based in vitro assays for L-NMMA acetate typically involve measuring the inhibition of NOS enzyme activity. In these assays, the enzyme (nNOS, eNOS, or iNOS) is incubated with its substrate, L-arginine, and cofactors in the presence of varying concentrations of L-NMMA acetate. The amount of NO produced is measured, often indirectly by quantifying the formation of the byproduct L-citrulline using radiometric or colorimetric methods. The Ki values are determined from these experiments.
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| Cell Assay |
Cellular assays for L-NMMA acetate involve treating cells that express NOS isoforms with the compound and measuring the inhibition of NO production. Cells are stimulated to produce NO, for example, with calcium ionophores (for eNOS and nNOS) or with LPS and cytokines (for iNOS). The concentration of nitrite/nitrate, the stable breakdown products of NO, in the culture supernatant is measured using the Griess reaction or chemiluminescence. The IC₅₀ or Ki for NOS inhibition is determined.
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| Animal Protocol |
In vivo animal models for L-NMMA acetate include studies in rodents to assess the effects of NOS inhibition on cardiovascular function. The compound is administered intravenously or intraperitoneally, and blood pressure, heart rate, and vascular resistance are measured. The compound is also used in models of septic shock to study the role of iNOS in hypotension. These studies help to elucidate the physiological roles of NO in vivo.
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| ADME/Pharmacokinetics |
L-NMMA acetate has a molecular weight of 248.28 g/mol and a molecular formula of C₉H₂₀N₄O₄. Its CAS number is 53308-83-1. The compound is a white to off-white solid. It is soluble in water. Purity is typically ≥98%. Storage conditions: -20°C, protected from light and moisture. The compound is supplied for research use only.
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| Toxicity/Toxicokinetics |
Detailed toxicological data for L-NMMA acetate are not extensively provided in the referenced sources. As a research compound, its safety profile would need to be established through standard preclinical toxicity assessments. The compound is intended for research use only and is not for human consumption. Standard laboratory safety precautions should be followed when handling the compound.
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| References |
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| Additional Infomation |
L-NMMA acetate is also known as NG-monomethyl-L-arginine acetate and Tilarginine acetate. It is a non-selective inhibitor of all three NOS isoforms (nNOS, eNOS, iNOS), with Ki values of approximately 0.18, 0.4, and 6 µM, respectively. It is a widely used research tool for studying the role of NO in various physiological and pathological processes. Its CAS number is 53308-83-1.
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| Molecular Formula |
C9H20N4O4
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|---|---|
| Molecular Weight |
248.28
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| Exact Mass |
248.148
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| CAS # |
53308-83-1
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| PubChem CID |
135242
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| Appearance |
White to off-white solid powder
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| Boiling Point |
392.7ºC at 760 mmHg
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| Melting Point |
180-190ºC
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| Flash Point |
191.3ºC
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| LogP |
0.595
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
17
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| Complexity |
224
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| Defined Atom Stereocenter Count |
1
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| SMILES |
CC(=O)O.CN=C(N)NCCC[C@@H](C(=O)O)N
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| InChi Key |
IKPNWIGTWUZCKM-JEDNCBNOSA-N
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| InChi Code |
InChI=1S/C7H16N4O2.C2H4O2/c1-10-7(9)11-4-2-3-5(8)6(12)13;1-2(3)4/h5H,2-4,8H2,1H3,(H,12,13)(H3,9,10,11);1H3,(H,3,4)/t5-;/m0./s1
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| Chemical Name |
acetic acid;(2S)-2-amino-5-[(N'-methylcarbamimidoyl)amino]pentanoic acid
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| Synonyms |
LNMMA acetate; L NMMA acetate; L-NMMA acetate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ≥ 50 mg/mL (~201.39 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (402.77 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.0277 mL | 20.1386 mL | 40.2771 mL | |
| 5 mM | 0.8055 mL | 4.0277 mL | 8.0554 mL | |
| 10 mM | 0.4028 mL | 2.0139 mL | 4.0277 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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