| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
|
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| Other Sizes |
| Targets |
l-Limonene does not have a specific pharmacological target. It is a natural product used as a solvent, flavoring agent, and in research. It has been shown to inhibit the proliferation of colon cancer cells in vitro.
|
|---|---|
| ln Vitro |
In vitro, l-Limonene has been shown to inhibit the proliferation of colon cancer cells. Its effects on cell proliferation and other cellular processes have been studied in various cell lines.
|
| ln Vivo |
In vivo, l-Limonene has been studied for its potential chemopreventive effects. However, specific in vivo activity data is not detailed in the provided search results.
|
| Enzyme Assay |
The in vitro activity of l-Limonene can be assessed using cell viability assays. Cancer cells are cultured in appropriate media and treated with various concentrations of l-Limonene. Cell viability is measured using standard assays such as MTT to determine its antiproliferative effects.
|
| Cell Assay |
For cellular assays, cancer cell lines, such as colon cancer cells, are used. Cells are treated with different concentrations of l-Limonene, and cell proliferation, apoptosis, and other cellular responses are assessed.
|
| Animal Protocol |
In vivo, l-Limonene is typically administered orally to animal models. Its effects on tumor growth and other parameters are assessed.
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| ADME/Pharmacokinetics |
Metabolism / Metabolites
(-)-Limonene's known human metabolites include carvacrol and perillol. l-Limonene has a molecular weight of 136.13 g/mol and a molecular formula of C10H16. It is a clear, colorless liquid with a characteristic odor. It is insoluble in water but soluble in organic solvents. |
| Toxicity/Toxicokinetics |
l-Limonene is generally recognized as safe (GRAS) for use as a food additive. It has low toxicity. However, it can cause skin and eye irritation in high concentrations.
|
| References | |
| Additional Infomation |
(4S)-Limonene is an optically active limonene with a (4S) configuration and is the enantiomer of (4R)-limonene. (-)-Limonene has been reported to exist in Pinus densiflora, Minthostachys mollis, and other organisms with relevant data. Limonene is a naturally occurring monoene that is a clear, colorless liquid at room temperature. It is the main component of orange oil and has a wide range of uses, including as a flavoring agent and spice. The U.S. Food and Drug Administration (FDA) has determined it to be safe for use in food. See also: Spearmint Oil (partial).
l-Limonene is a natural monoterpene with applications as a flavoring agent and in research. It is found in various citrus oils. It has been studied for its potential chemopreventive effects. It is not a pharmaceutical drug. |
| Molecular Formula |
C10H16
|
|---|---|
| Molecular Weight |
136.23
|
| Exact Mass |
136.125
|
| CAS # |
5989-54-8
|
| PubChem CID |
439250
|
| Appearance |
Colorless to light yellow liquid
|
| Density |
0.8±0.1 g/cm3
|
| Boiling Point |
175.4±20.0 °C at 760 mmHg
|
| Melting Point |
-104--84ºC
|
| Flash Point |
42.8±0.0 °C
|
| Vapour Pressure |
1.5±0.2 mmHg at 25°C
|
| Index of Refraction |
1.468
|
| LogP |
4.45
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
0
|
| Rotatable Bond Count |
1
|
| Heavy Atom Count |
10
|
| Complexity |
163
|
| Defined Atom Stereocenter Count |
1
|
| SMILES |
C=C(C)[C@@H]1CC=C(C)CC1
|
| InChi Key |
XMGQYMWWDOXHJM-SNVBAGLBSA-N
|
| InChi Code |
InChI=1S/C10H16/c1-8(2)10-6-4-9(3)5-7-10/h4,10H,1,5-7H2,2-3H3/t10-/m1/s1
|
| Chemical Name |
(4S)-1-methyl-4-prop-1-en-2-ylcyclohexene
|
| Synonyms |
beta-Limonene; (S)-Limonene; l-Limonene
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~734.05 mM)
H2O : ~50 mg/mL (~367.03 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (18.35 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (18.35 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (18.35 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 50 mg/mL (367.03 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 7.3405 mL | 36.7026 mL | 73.4053 mL | |
| 5 mM | 1.4681 mL | 7.3405 mL | 14.6811 mL | |
| 10 mM | 0.7341 mL | 3.6703 mL | 7.3405 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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