| Size | Price | |
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| Other Sizes |
| Targets |
L-Cystine diHCl does not have a specific pharmacological target. It is a nutrient and amino acid source used in cell culture media. As a precursor for glutathione synthesis, it plays a role in maintaining cellular redox balance. Cystine is also used to characterize and identify specific amino acid transport systems in cells, such as system Xc(-).
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|---|---|
| ln Vitro |
In vitro, L-Cystine diHCl is used as a cell culture media component for the commercial biomanufacture of therapeutic recombinant proteins and monoclonal antibodies. It is a sulfur-containing precursor for glutathione synthesis. Homeostasis of L-Cystine is important for glutathione function. It plays a key role in maintaining cellular redox balance.
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| ln Vivo |
In vivo, L-Cystine is a naturally occurring amino acid that plays a crucial role in the formation of proteins and other important biomolecules. It is a dimer of cysteine and is involved in sulfur transport. Cystine is also essential for oxygen production in arterial smooth muscle cells and the modification of low-density lipoprotein. Specific in vivo therapeutic applications are not detailed.
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| Enzyme Assay |
A cell-free assay for L-Cystine diHCl is not applicable as it is not an enzyme inhibitor. Its properties as an amino acid can be characterized by standard biochemical methods. It can be used as a substrate or standard in assays measuring amino acid content or transport.
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| Cell Assay |
Cellular experiments with L-Cystine diHCl typically involve its use as a component in cell culture media to support cell growth and protein production. It can also be used to study amino acid transport systems. Specific protocols depend on the cell type and experimental design.
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| Animal Protocol |
In vivo animal experiments for L-Cystine diHCl are not described in the available sources. As a nutritional supplement, it has been studied in animal models of nutrition and metabolism. However, specific protocols and data are not provided.
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| ADME/Pharmacokinetics |
L-Cystine diHCl has a molecular formula of C6H14Cl2N2O4S2 and a molecular weight of 313.22 g/mol. Its CAS number is 30925-07-6. It is a solid. For storage, it should be kept at room temperature in a cool and dark place (<15°C). Specific pharmacokinetic properties are not applicable as it is a nutrient rather than a therapeutic drug.
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| Toxicity/Toxicokinetics |
Toxicity data for L-Cystine diHCl are not provided in the available sources. As an amino acid, it is generally recognized as safe for its intended uses in cell culture and as a nutritional supplement. No specific toxicological concerns are reported.
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| References | |
| Additional Infomation |
L-Cystine diHCl (CAS: 30925-07-6) is the dihydrochloride salt of L-cystine. It is a sulfur-containing amino acid derivative used as a cell culture component and a precursor for glutathione synthesis. It is used in research related to protein structure, cell biology, and oxidative stress. It is not a therapeutic drug and is strictly for research purposes.
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| Molecular Formula |
C6H14CL2N2O4S2
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|---|---|
| Molecular Weight |
311.20648
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| Exact Mass |
311.977
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| CAS # |
30925-07-6
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| PubChem CID |
21121987
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| Appearance |
White to off-white solid powder
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| Boiling Point |
505.5ºC at 760 mmHg
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| Flash Point |
259.5ºC
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| LogP |
2.196
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| Hydrogen Bond Donor Count |
6
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
16
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| Complexity |
192
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| Defined Atom Stereocenter Count |
2
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| SMILES |
C([C@@H](C(=O)O)N)SSC[C@@H](C(=O)O)N.Cl
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| InChi Key |
IOCJWNPYGRVHLN-MMALYQPHSA-N
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| InChi Code |
InChI=1S/C6H12N2O4S2.ClH/c7-3(5(9)10)1-13-14-2-4(8)6(11)12;/h3-4H,1-2,7-8H2,(H,9,10)(H,11,12);1H/t3-,4-;/m0./s1
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| Chemical Name |
(2R)-2-amino-3-[[(2R)-2-amino-2-carboxyethyl]disulfanyl]propanoic acid;hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~159.63 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (3.99 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.2133 mL | 16.0663 mL | 32.1326 mL | |
| 5 mM | 0.6427 mL | 3.2133 mL | 6.4265 mL | |
| 10 mM | 0.3213 mL | 1.6066 mL | 3.2133 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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