| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
L-Anserine nitrate does not have a single defined molecular target but acts through multiple mechanisms. As a dipeptide, it functions as a physiological buffer, helping to maintain pH homeostasis in muscle tissue during intense physical activity. Its antioxidant activity is attributed to its ability to scavenge reactive oxygen species (ROS), particularly hydroxyl radicals, thereby protecting cells from oxidative damage. It also inhibits protein glycation, which is the non-enzymatic addition of sugars to proteins, a process implicated in aging and diabetic complications. These combined activities contribute to its neuroprotective and anti-inflammatory effects.
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| ln Vitro |
In vitro, L-Anserine nitrate exhibits potent antioxidant activity, effectively scavenging hydroxyl radicals in cell-free systems. It inhibits nonenzymatic protein glycation induced by reducing sugars, though it is much less potent than L-carnosine as an inhibitor of protein crosslinking. The compound has been shown to protect against neurotoxicity induced by endoplasmic reticulum (ER) stress inducers. These in vitro activities confirm its role as an antioxidant and cytoprotective agent.
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| ln Vivo |
Detailed in vivo activity data for L-Anserine nitrate are not extensively provided in the referenced sources. However, as a naturally occurring compound in skeletal muscle, it is believed to enhance exercise performance and recovery by buffering acidity and reducing oxidative stress. Its antioxidant and anti-inflammatory properties suggest potential for in vivo protective effects in models of neurodegenerative diseases and other conditions involving oxidative stress. Further studies are needed to fully characterize its in vivo efficacy.
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| Enzyme Assay |
Non-cell-based assays for L-Anserine nitrate typically involve measuring its antioxidant capacity. Common assays include the hydroxyl radical scavenging assay, where the compound's ability to neutralize hydroxyl radicals is measured using a fluorescent or colorimetric probe. The inhibition of protein glycation can be assessed in vitro by incubating the compound with a sugar (e.g., glucose or ribose) and a target protein (e.g., bovine serum albumin) and measuring the formation of advanced glycation end-products (AGEs).
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| Cell Assay |
Cellular assays for L-Anserine nitrate are performed using various cell lines to assess its cytoprotective and antioxidant effects. Cells are treated with the compound and then exposed to oxidative stressors, such as hydrogen peroxide or ER stress inducers. Cell viability is measured using standard assays like MTT, and markers of oxidative stress and apoptosis are assessed. These assays confirm the compound's ability to protect cells from damage.
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| Animal Protocol |
In vivo animal models for L-Anserine nitrate are not extensively detailed in the referenced sources. However, studies could involve animal models of exercise-induced muscle damage, where the compound is administered orally or intraperitoneally, and markers of muscle damage, inflammation, and oxidative stress are measured. Models of neurodegenerative diseases, such as Parkinson's or Alzheimer's, could also be used to assess its neuroprotective potential.
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| ADME/Pharmacokinetics |
L-Anserine nitrate has a molecular weight of 305.27 g/mol and a molecular formula of C₁₀H₁₇N₅O₆. Its CAS number is 10030-52-1. The compound is a white to off-white solid powder. It is soluble in water. Purity is typically ≥98%. Storage conditions: powder at -20°C. The compound is supplied for research use only. Detailed pharmacokinetic parameters are not extensively reported.
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| Toxicity/Toxicokinetics |
Detailed toxicological data for L-Anserine nitrate are not extensively provided in the referenced sources. As a naturally occurring dipeptide found in food sources, it is generally considered safe. The compound is intended for research use only and is not for human consumption. Standard laboratory safety precautions should be followed when handling the compound.
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| Additional Infomation |
L-Anserine nitrate is also known as L-Anserine nitrate salt and β-alanyl-3-methyl-L-histidine nitrate. It is a dipeptide found in most animal tissues with antioxidant, anti-cross-linking, and anti-glycation activities. It protects against neurotoxicity induced by ER-stress inducers. The compound is of interest in sports nutrition and supplements, potentially enhancing exercise performance and recovery. Its CAS number is 10030-52-1.
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| Molecular Formula |
C10H17N5O6
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|---|---|
| Molecular Weight |
303.27
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| Exact Mass |
303.117
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| CAS # |
10030-52-1
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| Related CAS # |
10030-52-1 (nitrate);584-85-0;
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| PubChem CID |
112071
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| Appearance |
White to off-white solid powder
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| Boiling Point |
611.3ºC at 760mmHg
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| Melting Point |
226-228ºC
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| Flash Point |
323.5ºC
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| Vapour Pressure |
8.52E-16mmHg at 25°C
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| LogP |
0.147
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
21
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| Complexity |
310
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| Defined Atom Stereocenter Count |
1
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| SMILES |
CN1C=NC=C1C[C@@H](C(=O)O)NC(=O)CCN.[N+](=O)(O)[O-]
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| InChi Key |
OLWOKAYJAHHSNY-QRPNPIFTSA-N
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| InChi Code |
InChI=1S/C10H16N4O3.HNO3/c1-14-6-12-5-7(14)4-8(10(16)17)13-9(15)2-3-11;2-1(3)4/h5-6,8H,2-4,11H2,1H3,(H,13,15)(H,16,17);(H,2,3,4)/t8-;/m0./s1
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| Chemical Name |
(2S)-2-(3-aminopropanoylamino)-3-(3-methylimidazol-4-yl)propanoic acid;nitric acid
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| Synonyms |
EINECS 233-079-7; L-Anserine nitrate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~250 mg/mL (~824.32 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 50 mg/mL (164.86 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.2974 mL | 16.4870 mL | 32.9739 mL | |
| 5 mM | 0.6595 mL | 3.2974 mL | 6.5948 mL | |
| 10 mM | 0.3297 mL | 1.6487 mL | 3.2974 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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