| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| Other Sizes |
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| ln Vitro |
The actinomycete Kitasatosporia kifunense 9482 is the source of the alkaloid kifunensine. It can inhibit N-glycan synthesis at the an8GlcNAc2 (Man8) or Man9GlcNAc2 (Man9) stage and is the most potent α-mannosidase I inhibitor [3].
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|---|---|
| Cell Assay |
RT-PCR[3]
Cell Types: hybridoma cells expressing human IgG1 monoclonal antibody [3]. Tested Concentrations: 2 micrograms/ml. Incubation Duration: 4 days. Experimental Results: Significant reduction in lentil lectin binding. Kifunensine was the most effective of the inhibitors tested in producing antibodies containing oligomannose residues but no fucose. |
| Animal Protocol |
Animal/Disease Models: BALB/c mouse[3].
Doses: 5 mg/kg. Route of Administration: intravenously (iv) (iv)(iv) via tail vein. Experimental Results: There were no significant differences in serum levels of anti-TEM B mAb produced by cells treated with or without kifunensine over a 7-day period. |
| References |
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| Additional Infomation |
Kifunensine has been reported in Kitasatospora kifunensis with data available.
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| Molecular Formula |
C8H12N2O6
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|---|---|
| Molecular Weight |
232.19068
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| Exact Mass |
232.069
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| CAS # |
109944-15-2
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| PubChem CID |
130611
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| Appearance |
White to off-white solid powder
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| Density |
1.9±0.1 g/cm3
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| Melting Point |
>280℃
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| Index of Refraction |
1.706
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| LogP |
-2.21
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
16
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| Complexity |
335
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| Defined Atom Stereocenter Count |
5
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| SMILES |
C([C@@H]1[C@H]([C@@H]([C@@H]([C@@H]2N1C(=O)C(=O)N2)O)O)O)O
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| InChi Key |
OIURYJWYVIAOCW-PQMKYFCFSA-N
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| InChi Code |
InChI=1S/C8H12N2O6/c11-1-2-3(12)4(13)5(14)6-9-7(15)8(16)10(2)6/h2-6,11-14H,1H2,(H,9,15)/t2-,3-,4+,5+,6+/m1/s1
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| Chemical Name |
(5R,6R,7S,8R,8aS)-6,7,8-trihydroxy-5-(hydroxymethyl)-1,5,6,7,8,8a-hexahydroimidazo[1,2-a]pyridine-2,3-dione
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~11.9 mg/mL (~51.25 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.19 mg/mL (5.13 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 11.9 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.19 mg/mL (5.13 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 11.9 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 1.19 mg/mL (5.13 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.3068 mL | 21.5341 mL | 43.0682 mL | |
| 5 mM | 0.8614 mL | 4.3068 mL | 8.6136 mL | |
| 10 mM | 0.4307 mL | 2.1534 mL | 4.3068 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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