| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Kahweol targets multiple signaling pathways. It blocks LPS-induced activation of NF-κB by preventing IκB degradation and inhibiting IκB kinase activity. Kahweol also activates AMP-activated protein kinase (AMPK), which inhibits adipogenesis and increases glucose uptake. Additionally, kahweol induces apoptosis.
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| ln Vitro |
In AML12 and LX2 cells, kahweol (20–40 μM; 24 hours) decreases TGF-β-induced CTGF protein production [1]. While Kahweol (20–40 μM; 1 hour) has no effect on expression in LX2 cells, it decreases TGF-β-induced phosphorylated Smad2/3 expression in AML12 cells [1]. In primary hepatocytes, kahweol (20–40 μM) only significantly lowers TGF-β-stimulated phospho-ERK and JNK expression [1]. In 3T3-L1 cells, kahweol (0-25 μg/ml; 6-24 hours) leads to a time-dependent increase in the phosphorylation of AMPK and its downstream target, acetyl-CoA carboxylase (ACC) [2]. Kahweol (0–25 μg/ml) regulates adipocyte development and lipid metabolism by substantially altering the protein expression of PPARγ, C/EBPα, FABP4, and FASN [2].
Kahweol has anti-inflammatory effects in RAW macrophages, inhibiting LPS-induced nitric oxide and prostaglandin E2 production with IC50 values of 54 and 23 μM, respectively. It inhibits adipogenesis and increases glucose uptake through AMPK activation. Kahweol also functions as an angiogenesis inhibitor and apoptosis inducer. |
| ln Vivo |
Specific in vivo efficacy data for kahweol are not extensively detailed in standard reference sources. Given its anti-inflammatory, anti-angiogenic, and anti-cancer activities, it has been investigated for potential therapeutic applications in cancer, inflammation, and metabolic disorders. Kahweol is a component of coffee and may contribute to the health benefits associated with coffee consumption.
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| Enzyme Assay |
Kahweol's anti-inflammatory activity can be assessed using in vitro enzyme assays. The inhibition of IκB kinase (IKK) activity is measured using recombinant IKK enzyme and its substrate (IκBα) in the presence of varying concentrations of kahweol. NF-κB activation can be studied using electrophoretic mobility shift assay (EMSA) or reporter gene assays in cell lysates.
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| Cell Assay |
Western Blot Analysis[2]
Cell Types: 3T3-L1 Cell Tested Concentrations: 0, 5, 10, 25 μg/ml Incubation Duration: Experimental Results: PPARγ, C/EBPα, FABP4 and FASN expression diminished in a dose-dependent manner. The cellular activity of kahweol is evaluated in macrophage cell lines such as RAW 264.7 cells. Cells are stimulated with LPS in the presence or absence of kahweol. Nitric oxide production is measured using the Griess assay, and prostaglandin E2 production is measured by ELISA. NF-κB activation is assessed by Western blot for IκB degradation and p65 nuclear translocation. |
| Animal Protocol |
Specific in vivo animal experimental protocols for kahweol are not extensively detailed in standard reference sources. For evaluating its anti-inflammatory or anti-cancer activity, kahweol would be administered orally or intraperitoneally to animal models of inflammation or cancer. Inflammatory markers, tumor growth, and angiogenesis would be assessed to evaluate efficacy.
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| ADME/Pharmacokinetics |
Kahweol has a molecular formula of C20H26O3 and a molecular weight of 314.42. It is a diterpene molecule. Specific pharmacokinetic parameters such as half-life, bioavailability, and plasma protein binding are not extensively detailed in standard reference sources.
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| Toxicity/Toxicokinetics |
The toxicity profile of kahweol is not extensively documented. As a natural product, it is generally considered safe at levels present in coffee consumption. Specific LD50 values and organ-specific toxicity data are not readily available.
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| References |
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| Additional Infomation |
Kahweol is a diterpenoid compound with the molecular formula C20H26O3, isolated from Arabica coffee beans (Coffea arabica). It possesses antioxidant, anti-inflammatory, anti-angiogenic, and anti-proliferative properties. It can function as an angiogenesis inhibitor, apoptosis inducer, antioxidant, anti-inflammatory agent, antitumor agent, and plant metabolite. Kahweol belongs to the diterpenoid class, furan class, organic heteropentane compound, tertiary alcohol, and primary alcohol. It has been reported to exist in Coffea congensis, Coffea sessiliflora, and other organisms with relevant data.
Kahweol (CAS# 6894-43-5) is a natural product isolated from coffee beans. It has anticarcinogenic, anti-angiogenic, and anti-inflammatory activities. Kahweol inhibits NF-κB activation, activates AMPK, and induces apoptosis. It is a research tool for studying inflammation, cancer, and metabolism. |
| Molecular Formula |
C20H26O3
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|---|---|
| Molecular Weight |
314.4186
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| Exact Mass |
314.188
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| CAS # |
6894-43-5
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| Related CAS # |
6894-43-5;81760-47-6;
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| PubChem CID |
114778
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| Appearance |
Off-white to light yellow solid powder
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
476.6±45.0 °C at 760 mmHg
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| Melting Point |
88-90 °C
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| Flash Point |
242.0±28.7 °C
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| Vapour Pressure |
0.0±1.3 mmHg at 25°C
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| Index of Refraction |
1.623
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| LogP |
3.38
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
23
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| Complexity |
545
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| Defined Atom Stereocenter Count |
6
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| SMILES |
C[C@@]12C=CC3=C([C@H]1CC[C@]45[C@H]2CC[C@H](C4)[C@](C5)(CO)O)C=CO3
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| InChi Key |
JEKMKNDURXDJAD-HWUKTEKMSA-N
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| InChi Code |
InChI=1S/C20H26O3/c1-18-7-5-16-14(6-9-23-16)15(18)4-8-19-10-13(2-3-17(18)19)20(22,11-19)12-21/h5-7,9,13,15,17,21-22H,2-4,8,10-12H2,1H3/t13-,15-,17+,18-,19+,20+/m1/s1
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| Chemical Name |
(1S,4S,12S,13R,16R,17R)-17-(hydroxymethyl)-12-methyl-8-oxapentacyclo[14.2.1.01,13.04,12.05,9]nonadeca-5(9),6,10-trien-17-ol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 33.33 mg/mL (~106.00 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.67 mg/mL (5.31 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 16.7 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.67 mg/mL (5.31 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 16.7 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. View More
Solubility in Formulation 3: 1.67 mg/mL (5.31 mM) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1805 mL | 15.9023 mL | 31.8046 mL | |
| 5 mM | 0.6361 mL | 3.1805 mL | 6.3609 mL | |
| 10 mM | 0.3180 mL | 1.5902 mL | 3.1805 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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