| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 50mg |
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| 100mg | |||
| 250mg | |||
| Other Sizes |
| Targets |
Mitochondrial Na⁺/Ca²⁺ exchanger (mNCX). ITH-12575 also reduces Ca²⁺ influx through CALHM1 channels at low micromolar concentrations. The compound regulates mitochondrial calcium homeostasis and is involved in neuronal excitability and calcium-dependent metabolism.
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|---|---|
| ln Vitro |
ITH-12575 reduces Ca²⁺ efflux from the mitochondria to the cytosol in HeLa cells with an EC₅₀ of 0.69 μM. It reduces Ca²⁺ influx through CALHM1 at low micromolar concentrations. The compound shows neuroprotective activity and protects against Ca²⁺ overload.
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| ln Vivo |
ITH-12575 has neuroprotective activity and was shown to protect against Ca²⁺ overload and mitigate neuronal damage in a model of oxidative stress. The compound is used to study mitochondrial signaling, neuronal excitability, calcium-dependent metabolism, and mechanisms of neurodegenerative diseases. Detailed in vivo efficacy data has been reported in neuroprotection models.
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| Enzyme Assay |
Mitochondrial calcium efflux assay: HeLa cells are loaded with a calcium-sensitive fluorescent dye and treated with ITH-12575 at varying concentrations. Mitochondrial and cytosolic calcium levels are measured using ratiometric fluorescence imaging. The EC₅₀ for inhibition of Ca²⁺ efflux from mitochondria to cytosol is calculated.
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| Cell Assay |
CALHM1 calcium influx assay: Cells expressing CALHM1 channels are treated with ITH-12575 at low micromolar concentrations. Ca²⁺ influx is measured using fluorescent calcium indicators (e.g., Fura-2 or Fluo-4). The reduction in Ca²⁺ influx is quantified, and dose-response curves are generated.
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| Animal Protocol |
Oxidative stress neuroprotection model: Neuronal cells or animal models are subjected to oxidative stress conditions. ITH-12575 is administered at various doses. Neuronal damage is assessed by cell viability assays, mitochondrial function measurements, and histological analysis. Protection against Ca²⁺ overload is evaluated.
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| ADME/Pharmacokinetics |
ITH-12575 has a molecular weight of 331.86 g/mol and molecular formula C₁₈H₁₈ClNOS. Purity is ≥98% by HPLC. The compound is stored as powder at -20°C for up to 3 years. Comprehensive PK parameters remain to be fully characterized.
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| Toxicity/Toxicokinetics |
No detailed toxicity data has been published in the available literature. ITH-12575 is for research use only and not for human therapeutic applications. Standard laboratory safety practices should be followed when handling this compound.
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| References | |
| Additional Infomation |
ITH-12575 is a potent and selective inhibitor of mitochondrial Na⁺/Ca²⁺ exchange (mNCX) with an EC₅₀ of 0.69 μM for reducing Ca²⁺ efflux from mitochondria to cytosol. It also reduces Ca²⁺ influx through CALHM1 at low micromolar concentrations. The compound has neuroprotective activity and is used to study mitochondrial signaling, neurodegeneration, and calcium-dependent metabolism. No clinical trials or approved上市 status have been reported.
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| Molecular Formula |
C18H18CLNOS
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|---|---|
| Molecular Weight |
331.858
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| Exact Mass |
331.079
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| Elemental Analysis |
C, 65.15; H, 5.47; Cl, 10.68; N, 4.22; O, 4.82; S, 9.66
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| CAS # |
1802013-08-6
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| PubChem CID |
124201848
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| Appearance |
White to off-white solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
484.5±45.0 °C at 760 mmHg
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| Flash Point |
246.8±28.7 °C
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| Vapour Pressure |
0.0±1.2 mmHg at 25°C
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| Index of Refraction |
1.604
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| LogP |
5.76
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
22
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| Complexity |
403
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC(C)C1=CC=CC=C1C2C3=C(C=CC(=C3)Cl)NC(=O)CS2
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| InChi Key |
RDMUJSJZBYAAEF-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C18H18ClNOS/c1-11(2)13-5-3-4-6-14(13)18-15-9-12(19)7-8-16(15)20-17(21)10-22-18/h3-9,11,18H,10H2,1-2H3,(H,20,21)
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| Chemical Name |
7-chloro-5-(2-propan-2-ylphenyl)-1,5-dihydro-4,1-benzothiazepin-2-one
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| Synonyms |
ITH12575; ITH 12575; ITH-12575
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~150.67 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (7.53 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0133 mL | 15.0666 mL | 30.1332 mL | |
| 5 mM | 0.6027 mL | 3.0133 mL | 6.0266 mL | |
| 10 mM | 0.3013 mL | 1.5067 mL | 3.0133 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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