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| 10mg |
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| 25mg |
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| 50mg |
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| ln Vitro |
Melanin accumulation is stimulated by isozolin in a dose-dependent way. Additionally, after 48 and 72 hours, 30 μM isosakuranetin progressively raised Tyr, TRP1, and TRP2 expression. Isosakuranetin decreased the number of cells at concentrations higher than 45 μM, according to the MTT experiment. Trypan blue staining revealed through additional study that isosakuranetin decreased the proliferation rate of B16 melanocytes but had no effect on their viability. Tyrosinase activity of B16 melanocytes treated with 15 and 30 μM Isosakuranetin for 72 hours rose significantly by 2 and 3.2 fold, respectively, compared with control cells [1].
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| ln Vivo |
In the dose range of 1.5 to 6 mg/kg, isosakuranetin increases the threshold for paw withdrawal in a dose-dependent manner. Isosakuranetin raised the paw withdrawal threshold with statistical significance between 30 and 40 minutes for the 3 mg/kg dose and between 30 and 60 minutes for the 6 mg/kg dose, according to post hoc analysis [2].
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| References |
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| Additional Infomation |
4'-methoxy-5,7-dihydroxyflavanone is a dihydroxyflavanone that is flavanone substituted by hydroxy groups at positions 5 and 7 and a methoxy group at position 4' (the 2S stereoisomer). It has a role as a plant metabolite. It is a dihydroxyflavanone, a monomethoxyflavanone, a member of 4'-methoxyflavanones and a (2S)-flavan-4-one. It is functionally related to a (S)-naringenin.
Isosakuranetin has been reported in Prunus serrulata var. pubescens, Populus szechuanica, and other organisms with data available. |
| Molecular Formula |
C16H14O5
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|---|---|
| Molecular Weight |
286.2794
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| Exact Mass |
286.084
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| CAS # |
480-43-3
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| PubChem CID |
160481
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| Appearance |
White to yellow solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
539.2±50.0 °C at 760 mmHg
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| Melting Point |
193-194ºC
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| Flash Point |
205.6±23.6 °C
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| Vapour Pressure |
0.0±1.5 mmHg at 25°C
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| Index of Refraction |
1.638
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| LogP |
3.84
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
21
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| Complexity |
377
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| Defined Atom Stereocenter Count |
1
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| SMILES |
COC1=CC=C(C=C1)[C@@H]2CC(=O)C3=C(C=C(C=C3O2)O)O
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| InChi Key |
HMUJXQRRKBLVOO-AWEZNQCLSA-N
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| InChi Code |
InChI=1S/C16H14O5/c1-20-11-4-2-9(3-5-11)14-8-13(19)16-12(18)6-10(17)7-15(16)21-14/h2-7,14,17-18H,8H2,1H3/t14-/m0/s1
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| Chemical Name |
(2S)-5,7-dihydroxy-2-(4-methoxyphenyl)-2,3-dihydrochromen-4-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~62.5 mg/mL (~218.32 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 20.83 mg/mL (72.76 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of clear DMSO stock solution of 208.3 mg/mL to 900 μL of corn oil and mix evenly. Solubility in Formulation 2: ≥ 2.08 mg/mL (7.27 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (7.27 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.4931 mL | 17.4654 mL | 34.9308 mL | |
| 5 mM | 0.6986 mL | 3.4931 mL | 6.9862 mL | |
| 10 mM | 0.3493 mL | 1.7465 mL | 3.4931 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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