| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| Other Sizes |
| Targets |
Isorhynchophylline targets multiple pathways. It inhibits monoamine oxidase, contributing to its antidepressant-like effects. It suppresses rat blood vessel contraction and counteracts angiotensin II's hypertensive effect. Its neuroprotective effects are likely mediated through its anti-inflammatory and antioxidant activities. It enhances cognitive function and protects against neuronal damage.
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| ln Vitro |
In vitro, Isorhynchophylline exhibits neuroprotective, anticancer, and antihypertensive activities. It suppresses blood vessel contraction. It has anti-inflammatory effects. Its ability to inhibit monoamine oxidase has been demonstrated. These activities are measured in various cell-based and tissue-based assays.
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| ln Vivo |
In vivo, Isorhynchophylline (10-40 mg/kg) exhibits antidepressant-like effects in mice. It lowers blood pressure and provides protection against ischemia-induced neuronal damage. It enhances cognitive function and protects against neuronal damage, showing potential in Alzheimer’s and Parkinson’s disease research.
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| Enzyme Assay |
In vitro non-cell enzyme assays for Isorhynchophylline typically involve measuring the inhibition of monoamine oxidase (MAO) activity. The compound is incubated with the enzyme and a substrate, and the production of the oxidized product is measured. Its effects on angiotensin-converting enzyme (ACE) could also be assessed in a cell-free system.
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| Cell Assay |
In vitro cell-based assays for Isorhynchophylline use neuronal cell lines to study its neuroprotective effects against various insults, such as oxidative stress or excitotoxicity. Its effects on cancer cell lines can be studied to assess its anticancer activity. Its anti-inflammatory activity is studied in immune cells by measuring cytokine production.
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| Animal Protocol |
In vivo animal studies for Isorhynchophylline employ models of depression (e.g., forced swim test), hypertension (e.g., spontaneously hypertensive rats), and neurodegeneration (e.g., models of Alzheimer's or Parkinson's disease). The compound is administered, and behavioral, physiological, and biochemical parameters are assessed.
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| ADME/Pharmacokinetics |
Isorhynchophylline has a molecular weight of 384.46 g/mol and a molecular formula of C₂₂H₂₈N₂O₄. It is an oxindole alkaloid. It is soluble in DMSO. As a small, lipophilic molecule, it is expected to be orally bioavailable and to cross the blood-brain barrier. Detailed pharmacokinetic data are available from preclinical studies.
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| Toxicity/Toxicokinetics |
The toxicity profile of Isorhynchophylline is not extensively detailed in the provided search results. As a natural product from Uncaria rhynchophylla, which has a history of use, it is generally considered to have a good safety profile. However, comprehensive toxicological studies are required for therapeutic development.
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| References |
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| Additional Infomation |
Isorhynchophylline is an indoleazine compound and a metabolite. It has been reported to exist in Uncaria sinensis, Uncaria macrophylla, and other organisms with relevant data. See also: Nepeta cataria (partial).
Isorhynchophylline is an oxindole alkaloid from Uncaria rhynchophylla with neuroprotective, anticancer, and antihypertensive properties. It exhibits antidepressant-like effects and enhances cognitive function. It is also known as (+)-Isorhynchophylline. It is not approved for clinical use and is intended for research purposes only. |
| Molecular Formula |
C22H28N2O4
|
|---|---|
| Molecular Weight |
384.4687
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| Exact Mass |
384.204
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| CAS # |
6859-01-4
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| PubChem CID |
3037048
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| Appearance |
White to off-white solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
447.6±25.0 °C at 760 mmHg
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| Melting Point |
114ºC
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| Flash Point |
224.5±23.2 °C
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| Vapour Pressure |
0.0±1.1 mmHg at 25°C
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| Index of Refraction |
1.677
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| LogP |
1.9
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
28
|
| Complexity |
663
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| Defined Atom Stereocenter Count |
4
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| SMILES |
CC[C@H]1CN2CC[C@@]3([C@@H]2C[C@@H]1/C(=C\OC)/C(=O)OC)C4=CC=CC=C4NC3=O
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| InChi Key |
DAXYUDFNWXHGBE-VKCGGMIFSA-N
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| InChi Code |
InChI=1S/C22H28N2O4/c1-4-14-12-24-10-9-22(17-7-5-6-8-18(17)23-21(22)26)19(24)11-15(14)16(13-27-2)20(25)28-3/h5-8,13-15,19H,4,9-12H2,1-3H3,(H,23,26)/b16-13+/t14-,15-,19-,22-/m0/s1
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| Chemical Name |
methyl (E)-2-[(3S,6'R,7'S,8'aS)-6'-ethyl-2-oxospiro[1H-indole-3,1'-3,5,6,7,8,8a-hexahydro-2H-indolizine]-7'-yl]-3-methoxyprop-2-enoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~260.10 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.75 mg/mL (7.15 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 27.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.75 mg/mL (7.15 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 27.5 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6010 mL | 13.0049 mL | 26.0098 mL | |
| 5 mM | 0.5202 mL | 2.6010 mL | 5.2020 mL | |
| 10 mM | 0.2601 mL | 1.3005 mL | 2.6010 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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