| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
|
||
| 10mg |
|
||
| 100mg | |||
| Other Sizes |
| Targets |
Isopropyl ferulate does not have a well-defined biological target. As an ester derivative of ferulic acid, it exhibits antioxidant activity by scavenging free radicals and chelating metal ions. Its anti-inflammatory activity may involve the inhibition of pro-inflammatory mediators. The compound may also act as a UV-protective agent by absorbing ultraviolet radiation. Its primary targets are reactive oxygen species and inflammatory pathways.
|
|---|---|
| ln Vitro |
In vitro studies demonstrate that Isopropyl ferulate exhibits antioxidant, anti-inflammatory, and UV-protective activities. The compound's antioxidant activity is assessed using DPPH, ABTS, or ORAC assays, where it scavenges free radicals. Its anti-inflammatory activity is evaluated in cell-based assays by measuring the inhibition of pro-inflammatory cytokine production (e.g., TNF-alpha, IL-6) in stimulated immune cells. The compound's UV-protective activity is assessed by measuring its ability to absorb UV radiation.
|
| ln Vivo |
In vivo activity of Isopropyl ferulate has not been extensively characterized in the available literature. As a natural product with antioxidant and anti-inflammatory properties, the compound has potential applications in cosmetics and pharmaceuticals. However, detailed in vivo efficacy data, including specific disease models and dosing regimens, are not extensively reported. The compound is primarily used as a research tool.
|
| Enzyme Assay |
In vitro assays for Isopropyl ferulate involve measuring its antioxidant, anti-inflammatory, and UV-protective activities. Antioxidant activity is assessed using DPPH, ABTS, or ORAC assays, where the compound's ability to scavenge free radicals is measured spectrophotometrically. Anti-inflammatory activity is evaluated in cell-based assays by measuring the inhibition of pro-inflammatory cytokine production (e.g., TNF-alpha, IL-6) in LPS-stimulated macrophages using ELISA. UV-protective activity is assessed by measuring the compound's UV absorption spectrum.
|
| Cell Assay |
Cellular assays for Isopropyl ferulate are conducted to evaluate its anti-inflammatory and antioxidant effects. Immune cells (e.g., macrophages) are treated with the compound and stimulated with LPS or other inflammatory stimuli. Cytokine production (e.g., TNF-alpha, IL-6, IL-1beta) is measured by ELISA or qPCR. Oxidative stress markers such as ROS levels are measured using fluorescent probes (e.g., DCFH-DA). Cell viability is assessed using MTT or similar assays to ensure that observed effects are not due to cytotoxicity.
|
| Animal Protocol |
In vivo animal studies for Isopropyl ferulate are limited. For anti-inflammatory studies, animal models of inflammation (e.g., carrageenan-induced paw edema) could be used. For antioxidant studies, models of oxidative stress could be employed. Animals would be treated with the compound via oral or topical administration at various doses. Inflammatory markers or oxidative stress markers would be assessed. However, specific in vivo study protocols are not extensively reported.
|
| ADME/Pharmacokinetics |
Pharmacokinetic properties of Isopropyl ferulate have not been extensively characterized. The compound has a molecular weight of 236.26 and a molecular formula of C13H1₆O4. It is soluble in chloroform, dichloromethane, ethyl acetate, DMSO, and acetone. The compound is typically stored at -20degC, protected from light. Detailed PK parameters such as half-life, bioavailability, and volume of distribution are not reported in the available literature.
|
| Toxicity/Toxicokinetics |
Toxicological data for Isopropyl ferulate are limited. The compound is not intended for human therapeutic use and is supplied for research purposes only. In cell-based assays, the compound is generally well-tolerated at concentrations used for activity studies. The compound is derived from natural sources and is considered to have low toxicity. Standard safety precautions should be followed when handling this compound.
|
| References | |
| Additional Infomation |
Isopropyl ferulate is an ester derivative of ferulic acid with antioxidant, anti-inflammatory, and UV-protective properties. It is an extract of Notopterygium incisum. The compound has potential applications in cosmetics, pharmaceuticals, and food preservation. It is not an FDA-approved drug and has no clinical indications. The compound is available in high purity (≥98%) and is typically stored at -20degC, protected from light.
|
| Molecular Formula |
C13H16O4
|
|---|---|
| Molecular Weight |
236.2637
|
| Exact Mass |
236.105
|
| CAS # |
59831-94-6
|
| PubChem CID |
6434600
|
| Appearance |
White to off-white solid
|
| Density |
1.145g/cm3
|
| Boiling Point |
358.2ºC at 760mmHg
|
| Flash Point |
131.9ºC
|
| Index of Refraction |
1.556
|
| Source |
Originated from plants: Compositae Kleinia odora (Forssk.) DC.
Originated from plants: Umbelliferae |
| LogP |
2.365
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
5
|
| Heavy Atom Count |
17
|
| Complexity |
273
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
O(C(/C(/[H])=C(\[H])/C1C([H])=C([H])C(=C(C=1[H])OC([H])([H])[H])O[H])=O)C([H])(C([H])([H])[H])C([H])([H])[H]
|
| InChi Key |
KBTWMQRNUHIETA-FNORWQNLSA-N
|
| InChi Code |
InChI=1S/C13H16O4/c1-9(2)17-13(15)7-5-10-4-6-11(14)12(8-10)16-3/h4-9,14H,1-3H3/b7-5+
|
| Chemical Name |
propan-2-yl (E)-3-(4-hydroxy-3-methoxyphenyl)prop-2-enoate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: ~100 mg/mL (~423.3 mM; with ultrasonication)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (10.58 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (10.58 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (10.58 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.2326 mL | 21.1631 mL | 42.3263 mL | |
| 5 mM | 0.8465 mL | 4.2326 mL | 8.4653 mL | |
| 10 mM | 0.4233 mL | 2.1163 mL | 4.2326 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
