| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 100mg |
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| 250mg | |||
| Other Sizes |
| ln Vitro |
Isomangiferin exhibited concentration-dependent antiviral activity against Herpes Simplex Virus (HSV) in vitro. In Vero cells (African green monkey kidney cells) infected with HSV-1, it inhibited viral replication and cytopathic effect (CPE) formation. At concentrations of 50 μg/mL, 100 μg/mL, and 200 μg/mL, the viral inhibition rates were 35%, 62%, and 87% respectively. The 50% inhibitory concentration (IC50) for HSV-1 was 86 μg/mL [1]
Against HSV-2 in Vero cells, Isomangiferin showed similar antiviral efficacy: 50 μg/mL inhibited viral replication by 31%, 100 μg/mL by 58%, and 200 μg/mL by 83%, with an IC50 of 92 μg/mL. It did not affect Vero cell viability at concentrations up to 200 μg/mL (MTT assay: >90% viability vs. control) [1] |
|---|---|
| Cell Assay |
Vero cell culture and HSV infection assay: Vero cells were cultured in RPMI 1640 medium supplemented with fetal bovine serum, seeded in 96-well plates (1×10⁴ cells/well), and incubated for 24 hours to adhere. Isomangiferin was dissolved in culture medium to prepare concentrations of 25-200 μg/mL. Cells were pretreated with the compound for 1 hour, then infected with HSV-1 or HSV-2 at a multiplicity of infection (MOI) of 0.1. After 72 hours of incubation, CPE was observed under a microscope, and viral inhibition rate was calculated by comparing CPE-positive wells with the virus control group. Cell viability was assessed by MTT assay [1]
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| References | |
| Additional Infomation |
Isomangin is a xanthonesone compound with the structure 9H-xanthon-9-one, substituted with hydroxyl groups at positions 1, 3, 6, and 7, and partially substituted with 1,5-dehydro-D-glucol at position 1. It possesses anti-HSV-1 activity and is a plant metabolite. It belongs to the flavonoid class, is a C-glycoside, and a polyphenol. Isomangin has been reported to exist in Rigidella, Hypericum ascyron, and other organisms with relevant data.
Isomangin is a natural polyphenol compound belonging to the flavonoid glycoside family[1][2] It is naturally found in plants of the genus Hypericum (e.g., Hypericum perforatum, Hypericum hirsutum) and can be isolated from the aerial parts of these plants[2] Its mechanism against HSV virus is thought to be blocking the virus from adsorbing to the host cell and inhibiting viral DNA replication, without direct virucidal activity (pre-incubation of the virus with this compound did not reduce the infectivity of the virus)[1] |
| Molecular Formula |
C19H18O11
|
|---|---|
| Molecular Weight |
422.3396
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| Exact Mass |
422.084
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| CAS # |
24699-16-9
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| PubChem CID |
5318597
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| Appearance |
Light yellow to green yellow solid powder
|
| Density |
1.8±0.1 g/cm3
|
| Boiling Point |
798.8±60.0 °C at 760 mmHg
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| Flash Point |
288.2±26.4 °C
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| Vapour Pressure |
0.0±3.0 mmHg at 25°C
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| Index of Refraction |
1.789
|
| LogP |
0.13
|
| Hydrogen Bond Donor Count |
8
|
| Hydrogen Bond Acceptor Count |
11
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
30
|
| Complexity |
646
|
| Defined Atom Stereocenter Count |
5
|
| SMILES |
C1=C2C(=CC(=C1O)O)OC3=C(C2=O)C(=CC(=C3[C@H]4[C@@H]([C@H]([C@@H]([C@H](O4)CO)O)O)O)O)O
|
| InChi Key |
CDYBOKJASDEORM-HBVDJMOISA-N
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| InChi Code |
InChI=1S/C19H18O11/c20-4-11-15(26)16(27)17(28)19(30-11)13-9(24)2-8(23)12-14(25)5-1-6(21)7(22)3-10(5)29-18(12)13/h1-3,11,15-17,19-24,26-28H,4H2/t11-,15-,16+,17-,19+/m1/s1
|
| Chemical Name |
1,3,6,7-tetrahydroxy-4-[(2S,3R,4R,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]xanthen-9-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~118.39 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.92 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.92 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.3678 mL | 11.8388 mL | 23.6776 mL | |
| 5 mM | 0.4736 mL | 2.3678 mL | 4.7355 mL | |
| 10 mM | 0.2368 mL | 1.1839 mL | 2.3678 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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