| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| ln Vitro |
- Isomaltose was used as a specific substrate to determine the activity of α-1,6-glucosidase in leucocytes from healthy controls and patients with late-onset glycogenosis type II (GSD II). In healthy controls, the α-1,6-glucosidase activity toward Isomaltose was 4.2±0.8 nmol/h/mg protein. In patients with late-onset GSD II, this enzyme activity was significantly reduced, ranging from 0.3 to 1.1 nmol/h/mg protein (P<0.01 vs. healthy controls) [1]
- Isomaltose did not interfere with the activity of α-1,4-glucosidase (measured using maltose as the substrate), confirming its specificity for α-1,6-glucosidase without cross-reactivity with α-1,4-glucosidase [1] |
|---|---|
| Enzyme Assay |
- For α-1,6-glucosidase activity assay with Isomaltose: Leucocytes were isolated from venous blood via density gradient centrifugation and lysed in a buffer containing Triton X-100. The reaction mixture was prepared by combining the leucocyte lysate (serving as the enzyme source), Isomaltose (final concentration 50 mM, as the substrate), and sodium acetate buffer (pH 4.0) to a total volume of 0.2 mL. The mixture was incubated at 37°C for 2 hours. The reaction was terminated by adding 0.8 mL of 10% trichloroacetic acid, and the mixture was centrifuged to remove precipitated proteins. The amount of glucose released from Isomaltose hydrolysis was measured using the glucose oxidase-peroxidase method. Enzyme activity was expressed as nanomoles of glucose produced per hour per milligram of protein (nmol/h/mg protein) [1]
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| References | |
| Additional Infomation |
Isomaltose is a glycosyl glucose composed of two D-glucanose units linked by an α-(1-6) glycosidic bond. It is a metabolite, found in both humans and mice. Isomaltose is present in or produced by Escherichia coli (strains K12 and MG1655). It has been reported as an arm-shaped sugar in Drosophila melanogaster, Arabidopsis thaliana, and other organisms with relevant data. Isomaltose is present in or produced by Saccharomyces cerevisiae. Isomaltose is a disaccharide composed of two glucose units linked by an α-(1-6) glycosidic bond.
- Isomaltose (6-O-α-D-glucopyranosyl-D-glucose) is a disaccharide that is a specific substrate for α-1,6-glucosidase because it can only be hydrolyzed by this enzyme (not by other glucosidases, such as... α-1,4-glucosidase)[1] - In this study, isomaltose was not used as a therapeutic agent, but as a diagnostic tool: it helps identify late-onset type II glycogen storage disease by detecting a decrease in α-1,6-glucosidase activity in the leukocytes of patients, which is a key diagnostic feature of the disease[1] |
| Molecular Formula |
C12H22O11
|
|---|---|
| Molecular Weight |
342.3
|
| Exact Mass |
342.116
|
| CAS # |
499-40-1
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| PubChem CID |
439193
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| Appearance |
White to off-white solid powder
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| Density |
1.68±0.1 g/cm3 (20 ºC 760 Torr)
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| Boiling Point |
774.5±60.0 °C at 760 mmHg
|
| Melting Point |
120 ºC
|
| Flash Point |
288.9±26.4 °C
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| Vapour Pressure |
0.0±6.0 mmHg at 25°C
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| Index of Refraction |
1.623
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| LogP |
-4.34
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| Hydrogen Bond Donor Count |
8
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| Hydrogen Bond Acceptor Count |
11
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
23
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| Complexity |
382
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| Defined Atom Stereocenter Count |
9
|
| SMILES |
C([C@@H]1[C@H]([C@@H]([C@H]([C@H](O1)OC[C@@H]2[C@H]([C@@H]([C@H](C(O2)O)O)O)O)O)O)O)O
|
| InChi Key |
DLRVVLDZNNYCBX-RTPHMHGBSA-N
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| InChi Code |
InChI=1S/C12H22O11/c13-1-3-5(14)8(17)10(19)12(23-3)21-2-4-6(15)7(16)9(18)11(20)22-4/h3-20H,1-2H2/t3-,4-,5-,6-,7+,8+,9-,10-,11?,12+/m1/s1
|
| Chemical Name |
(3R,4S,5S,6R)-6-[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxymethyl]oxane-2,3,4,5-tetrol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~150 mg/mL (~438.21 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 50 mg/mL (146.07 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9214 mL | 14.6071 mL | 29.2141 mL | |
| 5 mM | 0.5843 mL | 2.9214 mL | 5.8428 mL | |
| 10 mM | 0.2921 mL | 1.4607 mL | 2.9214 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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