| Size | Price | Stock | Qty |
|---|---|---|---|
| 5g |
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| Other Sizes |
| Targets |
Isohomovanillic acid is a metabolite of dopamine and other catecholamines. It does not have a specific therapeutic target but serves as a biomarker for catecholamine metabolism. It is a member of the phenylacetic acids class and is a 4-O-methyl ether of (3,4-dihydroxyphenyl)acetic acid.
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|---|---|
| ln Vitro |
Isohomovanillic acid is an endogenous metabolite and is not known to have direct pharmacological activity. Its primary use is as a research compound and biomarker. It is not found in appreciable values in many normal human urines.
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| ln Vivo |
In vivo, Isohomovanillic acid serves as a metabolic product of catecholamine metabolism. It is extracted from urine at pH 2 by ethyl acetate. The compound is used in Parkinson's disease research as a biomarker.
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| Enzyme Assay |
Characterization of Isohomovanillic acid involves standard analytical techniques such as HPLC and mass spectrometry. The compound can be isolated from urine. Its role as a metabolite is confirmed through metabolic pathway analysis.
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| Cell Assay |
Cell-based studies for Isohomovanillic acid are not typically performed as it is a metabolite rather than a pharmacologically active compound. Its detection and quantification in biological samples are the primary research applications.
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| Animal Protocol |
In vivo studies involve the detection and quantification of Isohomovanillic acid in urine as a measure of catecholamine metabolism. The compound is used as a biomarker in Parkinson's disease research.
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| ADME/Pharmacokinetics |
Isohomovanillic acid has a molecular weight of 182.17 and a molecular formula of C9H10O4. Storage recommendations include standard conditions for research metabolites. The compound is soluble in organic solvents and can be extracted from urine.
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| Toxicity/Toxicokinetics |
Isohomovanillic acid is an endogenous metabolite and is not considered toxic at physiological levels. As a research compound, standard laboratory safety precautions should be followed when handling the compound.
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| References | |
| Additional Infomation |
Isohirovanillic acid is a phenylacetic acid compound, specifically the 4-O-methyl ether of (3,4-dihydroxyphenyl)acetic acid. It is a metabolite. It is an aromatic ether belonging to the phenolic and phenylacetic acid classes. Its function is related to that of (3,4-dihydroxyphenyl)acetic acid.
Isohomovanillic acid is a deaminated metabolite of catecholamines formed by catechol-O-methyltransferase. It is used in Parkinson's disease research and is a valuable compound for studying catecholamine metabolism. Synonyms include 3-Hydroxy-4-methoxyphenylacetic acid. |
| Molecular Formula |
C9H10O4
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|---|---|
| Molecular Weight |
182.1733
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| Exact Mass |
182.057
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| CAS # |
1131-94-8
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| PubChem CID |
160562
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| Appearance |
Off-white to light brown solid powder
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
371.3±27.0 °C at 760 mmHg
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| Melting Point |
128 °C
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| Flash Point |
153.1±17.2 °C
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| Vapour Pressure |
0.0±0.9 mmHg at 25°C
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| Index of Refraction |
1.573
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| LogP |
0.47
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
13
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| Complexity |
181
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
BWXLCOBSWMQCGP-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C9H10O4/c1-13-8-3-2-6(4-7(8)10)5-9(11)12/h2-4,10H,5H2,1H3,(H,11,12)
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| Chemical Name |
2-(3-hydroxy-4-methoxyphenyl)acetic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~548.94 mM)
H2O : ~6.67 mg/mL (~36.61 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (13.72 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (13.72 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (13.72 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.4894 mL | 27.4469 mL | 54.8938 mL | |
| 5 mM | 1.0979 mL | 5.4894 mL | 10.9788 mL | |
| 10 mM | 0.5489 mL | 2.7447 mL | 5.4894 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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