| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
Beta2-adrenergic receptor; Isoetharine mesylate is a selective agonist of the beta2-adrenergic receptor. Activation of this receptor in the bronchial smooth muscle leads to an increase in intracellular cAMP, which causes muscle relaxation and bronchodilation. It is a catecholamine that is methylated by catechol O-methyltransferase (COMT).
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| ln Vitro |
In HepG2 human hepatoma cells, isoetharine mesylate (50 μM, 18 hours) stimulates the synthesis and release of [35S] sulfated metabolites of catechol medications [1].
In vitro, Isoetharine mesylate acts as a beta2-adrenergic receptor agonist. It stimulates the production of cAMP in cells expressing the receptor. It is used in cell-based assays to study beta-adrenergic receptor signaling and smooth muscle relaxation. |
| ln Vivo |
Isoetharine mesylate fully suppresses pigment formation at 7.50 mM and inhibits melanin deposition with an AC50 value of 5.10 mM in the zebrafish larval model [2].
In vivo, Isoetharine mesylate is used as a bronchodilator to provide quick relief for bronchospasm and asthma. It is administered via inhalation. It relaxes the smooth muscles of the airways, improving airflow and reducing symptoms of respiratory distress. |
| Enzyme Assay |
In vitro, the binding affinity and agonist activity of Isoetharine mesylate are assessed using cell lines expressing the beta2-adrenergic receptor. Cells are treated with the compound, and cAMP production is measured. The compound is typically dissolved in DMSO or water.
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| Cell Assay |
In cell-based assays, cells expressing the beta2-adrenergic receptor are treated with Isoetharine mesylate. The compound is added to the culture medium. After incubation, intracellular cAMP levels are measured using ELISA or other assays. The compound's ability to stimulate cAMP production is quantified.
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| Animal Protocol |
In vivo, Isoetharine mesylate is administered to animal models via inhalation or injection. The dosage and route of administration depend on the specific model. Efficacy is evaluated by measuring airway resistance, bronchial relaxation, and other respiratory parameters.
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| ADME/Pharmacokinetics |
Isoetharine mesylate has a molecular weight of 335.42 and formula C14H25NO6S. It is soluble in water. Storage: Store at room temperature.
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| Toxicity/Toxicokinetics |
Specific toxicity data are documented from clinical use. Common side effects include tachycardia, tremor, and nervousness. It is for research use only and not for human therapeutic use outside approved indications.
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| References |
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| Additional Infomation |
β2-adrenergic agonists are used as bronchodilators to treat emphysema, bronchitis, and asthma.
Isoetharine mesylate is a selective beta2-adrenergic receptor agonist used as a bronchodilator. It is also known as isoetarine mesilate. |
| Molecular Formula |
C14H25NO6S
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|---|---|
| Molecular Weight |
335.41600
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| Exact Mass |
335.14
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| CAS # |
7279-75-6
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| Related CAS # |
Isoetharine;530-08-5;Isoetharine hydrochloride;50-96-4
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| PubChem CID |
23702
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| Appearance |
White to off-white solid powder
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| Boiling Point |
429.3ºC at 760 mmHg
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| Flash Point |
156.7ºC
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| LogP |
2.883
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
22
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| Complexity |
315
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
SOYAGMVKMXZVNZ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C13H21NO3.CH4O3S/c1-4-10(14-8(2)3)13(17)9-5-6-11(15)12(16)7-9;1-5(2,3)4/h5-8,10,13-17H,4H2,1-3H3;1H3,(H,2,3,4)
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| Chemical Name |
4-[1-hydroxy-2-(propan-2-ylamino)butyl]benzene-1,2-diol;methanesulfonic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~125 mg/mL (~372.67 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (6.20 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (6.20 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (6.20 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9813 mL | 14.9067 mL | 29.8134 mL | |
| 5 mM | 0.5963 mL | 2.9813 mL | 5.9627 mL | |
| 10 mM | 0.2981 mL | 1.4907 mL | 2.9813 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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