| Size | Price | Stock | Qty |
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| 25mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
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| ln Vitro |
For Huh-7, Hep-G2, SNU-449, and SNU-387, isocorydine (0-400 ug/ml; 48 hours) significantly reduces the IC50 for both ICD and DOX; the corresponding CI values are 0.605, 0.644, 0.804, and 0.707[1]. In human HCC cell lines, isocorydine (0-400 ug/ml; 48 hours) blocks the overexpression of mesenchymal markers and the downregulation of epithelial markers caused by DOX[1].
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| ln Vivo |
While isocorydine (0.4 mg/kg intraperitoneal injection given every two days for two weeks) slows the growth of tumors, the combination of isocorydine and doxorubicin (DOX) treatment greatly slows tumor growth[1].
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| Cell Assay |
Cell Viability Assay[1]
Cell Types: Huh-7, Hep-G2, SNU-387, SNU-449 cells Tested Concentrations: 0-400 ug/ml Incubation Duration: 24 hrs (hours) Experimental Results: Had a higher cytotoxicity in HCC cells in comparison to ICD or DOX alone. Western Blot Analysis [1] Cell Types: Huh-7, Hep-G2, SNU-387, SNU-449 cells Tested Concentrations: Incubation Duration: 24 hrs (hours) Experimental Results: Downregulated protein levels of Claudin-1 and E-cadherin. |
| Animal Protocol |
Animal/Disease Models: Female nude mice[1]
Doses: 0.4 mg/ml Route of Administration: Injected intraperitoneally every 2 days for 2 weeks Experimental Results: Combined treatment of isocorydine and DOX demonstrated a promising potential to eradicate HCC. |
| Toxicity/Toxicokinetics |
Toxicity Summary
Isocorydine induces G2/M phase arrest by increasing cyclin B1 and p-CDK1 expression levels, which was caused by decreasing the expression and inhibiting the activation of Cdc25C. The phosphorylation levels of Chk1 and Chk2 were increased after ICD treatment. Furthermore, G2/M arrest induced by ICD can be disrupted by Chk1 siRNA but not by Chk2 siRNA. In addition, isocorydine treatment led to a decrease in the percentage of CD133+ PLC/PRF/5 cells. Interestingly, isocorydine treatment dramatically decreased the tumorigenicity of SMMC-7721 and Huh7 cells. (A15211) |
| References | |
| Additional Infomation |
Isocorydine is an aporphine alkaloid.
Isocorydine has been reported in Pteridophyllum racemosum, Stephania lincangensis, and other organisms with data available. (S)-Isocorydine is found in cherimoya. (S)-Isocorydine is an alkaloid from Peumus boldus (boldo). (S)-Isocorydine belongs to the family of Aporphines. These are quinoline alkaloids containing the dibenzo[de,g]quinoline ring system. See also: Peumus boldus leaf (part of). |
| Molecular Formula |
CHEMICALFORMULAC20H23NO4
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|---|---|
| Molecular Weight |
341.4
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| Exact Mass |
341.162
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| CAS # |
475-67-2
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| PubChem CID |
10143
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| Appearance |
White to off-white solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
506.1±50.0 °C at 760 mmHg
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| Melting Point |
216-220ºC(lit.)
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| Flash Point |
259.9±30.1 °C
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| Vapour Pressure |
0.0±1.4 mmHg at 25°C
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| Index of Refraction |
1.604
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| LogP |
3.09
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
25
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| Complexity |
475
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| Defined Atom Stereocenter Count |
1
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| SMILES |
CN1CCC2=CC(=C(C3=C2[C@@H]1CC4=C3C(=C(C=C4)OC)O)OC)OC
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| InChi Key |
QELDJEKNFOQJOY-ZDUSSCGKSA-N
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| InChi Code |
InChI=1S/C20H23NO4/c1-21-8-7-12-10-15(24-3)20(25-4)18-16(12)13(21)9-11-5-6-14(23-2)19(22)17(11)18/h5-6,10,13,22H,7-9H2,1-4H3/t13-/m0/s1
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| Chemical Name |
(6aS)-1,2,10-trimethoxy-6-methyl-5,6,6a,7-tetrahydro-4H-dibenzo[de,g]quinolin-11-ol
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| Synonyms |
d-Isocorydine; Luteanin; Isocorydine
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~20 mg/mL (~58.58 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (7.32 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (7.32 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. View More
Solubility in Formulation 3: ≥ 2 mg/mL (5.86 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9291 mL | 14.6456 mL | 29.2912 mL | |
| 5 mM | 0.5858 mL | 2.9291 mL | 5.8582 mL | |
| 10 mM | 0.2929 mL | 1.4646 mL | 2.9291 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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