| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
|
||
| 10mg |
|
||
| 25mg |
|
||
| 50mg |
|
||
| 100mg |
|
||
| Other Sizes |
| Targets |
Viral enzymes; Isatin-β-thiosemicarbazone is an antiviral agent that targets multiple viral pathways. It is a potent inhibitor of poxviruses and herpes simplex viruses. Its exact mechanism of action involves interference with viral replication processes.
|
|---|---|
| ln Vitro |
In vitro, Isatin-β-thiosemicarbazone exhibits potent antiviral activity against poxviruses and HSV. It is used in antiviral assays to study its efficacy against various viral strains.
|
| ln Vivo |
In vivo, Isatin-β-thiosemicarbazone has potential for the treatment of poxvirus and HSV infections. It is an effective anti-poxvirus agent for the treatment of monkeypox, orthopox, and cowpox viruses. In vivo studies would be required to evaluate its efficacy.
|
| Enzyme Assay |
In vitro, antiviral activity is assessed using cell culture models infected with specific viruses. The compound is serially diluted and added to the infected cells. Viral replication is measured using plaque assays, qPCR, or other methods to determine the EC50.
|
| Cell Assay |
In cell-based assays, virus-infected cells are treated with Isatin-β-thiosemicarbazone. The compound is typically dissolved in DMSO. Viral replication and cell viability are measured to assess antiviral efficacy and cytotoxicity.
|
| Animal Protocol |
In vivo, Isatin-β-thiosemicarbazone is administered to animal models of viral infection. The dosage and administration route depend on the specific virus and model. Efficacy is evaluated by measuring viral load, survival, and clinical signs of infection.
|
| ADME/Pharmacokinetics |
Isatin-β-thiosemicarbazone has a molecular weight of 220.25 and formula C9H8N4OS. It is soluble in DMSO. Purity: >99%. Storage: Store at -20°C.
|
| Toxicity/Toxicokinetics |
Specific toxicity data are not extensively documented. The compound is for research use only. It should be handled with appropriate safety precautions.
|
| References | |
| Additional Infomation |
Isatin-β-thiosemicarbazone is a potent anti-poxvirus and anti-HSV agent. It interferes with multiple viral pathways.
|
| Molecular Formula |
C9H8N4OS
|
|---|---|
| Molecular Weight |
220.25102
|
| Exact Mass |
220.041
|
| CAS # |
27830-79-1
|
| PubChem CID |
690109
|
| Appearance |
Light yellow to yellow solid powder
|
| LogP |
2.1
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
1
|
| Heavy Atom Count |
15
|
| Complexity |
283
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
NC(NN=C1C2=CC=CC=C2NC1=O)=S
|
| InChi Key |
PEYNLAQGUNUQLY-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C9H8N4OS/c10-9(15)13-12-7-5-3-1-2-4-6(5)11-8(7)14/h1-4,11,14H,(H2,10,15)
|
| Chemical Name |
(2-hydroxy-1H-indol-3-yl)iminothiourea
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~125 mg/mL (~567.54 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (9.44 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.5403 mL | 22.7015 mL | 45.4030 mL | |
| 5 mM | 0.9081 mL | 4.5403 mL | 9.0806 mL | |
| 10 mM | 0.4540 mL | 2.2701 mL | 4.5403 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
