| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| 10mg |
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| 100mg | |||
| Other Sizes |
| ln Vitro |
- Proliferative activity on breast cancer cells: Iristectorin A exhibits proliferative activity on two human breast cancer cell lines, MCF-7 and MDA-MB-231. At concentrations of 1 μM, 5 μM, and 10 μM, it significantly increases the cell viability of MCF-7 cells by 12.5%, 28.3%, and 35.7% respectively compared to the control group. For MDA-MB-231 cells, the compound increases cell viability by 10.2%, 24.6%, and 31.4% at the same concentrations. No cytotoxicity is observed at the tested concentrations [1]
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|---|---|
| Cell Assay |
- Breast cancer cell proliferation assay: MCF-7 and MDA-MB-231 cells are seeded in 96-well plates at a density of 5×10³ cells per well and incubated overnight to allow attachment. Iristectorin A is dissolved in an appropriate solvent and added to the wells at final concentrations of 1 μM, 5 μM, and 10 μM. The control group receives the same volume of solvent without the compound. Cells are incubated at 37°C in a humidified atmosphere with 5% CO₂ for 72 hours. After incubation, a cell viability assay reagent is added to each well, and the plates are incubated for an additional 4 hours. The absorbance is measured at 490 nm using a microplate reader, and the cell viability rate is calculated relative to the control group [1]
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| References | |
| Additional Infomation |
Irisin A is reported to exist in plants of the genus Iris (such as Iris kemaonensis and Iris domestica) and other organisms with relevant data.
- Irisin A is a phenolic compound isolated from the rhizome of the Thai medicinal plant Belamcanda chinensis [1] - This study aimed to investigate the proliferative activity of phenolic compounds in Belamcanda chinensis and found that Irisin A is one of the active ingredients that has a proliferative effect on breast cancer cell lines [1] |
| Molecular Formula |
C23H24O12
|
|---|---|
| Molecular Weight |
492.4295
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| Exact Mass |
492.126
|
| CAS # |
37744-61-9
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| PubChem CID |
11968629
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| Appearance |
Off-white to light yellow solid powder
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| Density |
1.6±0.1 g/cm3
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| Boiling Point |
813.6±65.0 °C at 760 mmHg
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| Flash Point |
281.1±27.8 °C
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| Vapour Pressure |
0.0±3.1 mmHg at 25°C
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| Index of Refraction |
1.676
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| LogP |
-0.08
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| Hydrogen Bond Donor Count |
6
|
| Hydrogen Bond Acceptor Count |
12
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| Rotatable Bond Count |
6
|
| Heavy Atom Count |
35
|
| Complexity |
776
|
| Defined Atom Stereocenter Count |
5
|
| SMILES |
COC1=C(C=C(C=C1)C2=COC3=CC(=C(C(=C3C2=O)O)OC)O[C@H]4[C@@H]([C@H]([C@@H]([C@H](O4)CO)O)O)O)O
|
| InChi Key |
HFODKTZIQVSGJO-ZTATXHNCSA-N
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| InChi Code |
InChI=1S/C23H24O12/c1-31-12-4-3-9(5-11(12)25)10-8-33-13-6-14(22(32-2)19(28)16(13)17(10)26)34-23-21(30)20(29)18(27)15(7-24)35-23/h3-6,8,15,18,20-21,23-25,27-30H,7H2,1-2H3/t15-,18-,20+,21-,23-/m1/s1
|
| Chemical Name |
5-hydroxy-3-(3-hydroxy-4-methoxyphenyl)-6-methoxy-7-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxychromen-4-one
|
| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~203.07 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.08 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.08 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.0307 mL | 10.1537 mL | 20.3075 mL | |
| 5 mM | 0.4061 mL | 2.0307 mL | 4.0615 mL | |
| 10 mM | 0.2031 mL | 1.0154 mL | 2.0307 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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