| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
|
||
| 10mg |
|
||
| 25mg |
|
||
| 50mg |
|
||
| 100mg |
|
||
| 250mg | |||
| 500mg | |||
| Other Sizes |
| Targets |
INVIVO35833 specifically targets the Oct3/4 transcription factor, a core component of the pluripotency network. By promoting its expression and stabilization, the compound enhances Oct3/4's transcriptional activity in various human cells. This mechanism is crucial for its role in cell reprogramming and differentiation studies. It is also referred to as an Oct-4 activator.
|
|---|---|
| ln Vitro |
In vitro, INVIVO35833 demonstrates its activity by promoting the expression and stabilization of Oct3/4 in diverse human somatic cells. Its primary function is to enhance the transcriptional activity of Oct3/4, which is a key step in maintaining or inducing a pluripotent state. This makes it a valuable tool for studying the mechanisms of pluripotency and for improving the efficiency of induced pluripotent stem cell (iPSC) generation.
|
| ln Vivo |
In vivo, INVIVO35833 is used in research to study the effects of Oct3/4 activation. While the compound itself is not administered as a therapeutic, its mechanism of enhancing Oct3/4 activity is relevant for understanding stem cell biology and developing cell-based therapies. By promoting Oct3/4 expression, it can influence the differentiation and reprogramming of cells in vivo.
|
| Enzyme Assay |
In vitro enzyme/receptor binding studies are not applicable to INVIVO35833, as its target is a transcription factor, not an enzyme or a receptor. Its activity is assessed by measuring its effects on gene expression. In these studies, cells are treated with the compound, and the levels of Oct3/4 mRNA and protein are measured by qRT-PCR and Western blotting, respectively. The transcriptional activity of Oct3/4 is assessed using reporter assays.
|
| Cell Assay |
In vitro cellular assays for INVIVO35833 are the primary method for evaluating its activity. In these experiments, various human somatic cells are treated with the compound. The key readouts include the expression levels of Oct3/4 and its target genes, which are measured by qRT-PCR or Western blotting. Additionally, the effect of the compound on cell differentiation or reprogramming efficiency is assessed, particularly in the context of iPSC generation.
|
| Animal Protocol |
In vivo animal studies for INVIVO35833 are not extensively documented in the provided sources. However, as a tool for stem cell biology, its effects could be studied in animal models of regenerative medicine. For example, it could be used to assess whether enhancing Oct3/4 activity in vivo can promote tissue regeneration or improve the outcome of cell transplantation. Such studies would involve administering the compound and analyzing the resulting cellular and tissue changes.
|
| ADME/Pharmacokinetics |
Pharmacokinetic properties of INVIVO35833 are indicated by its molecular weight of 241.27 g/mol and a molecular formula of C₁₅H₁₂FNO. It is typically a solid powder and is soluble in DMSO. For storage, it is recommended to keep the powder at -20°C to maintain stability. These properties are important for its use in cell culture and for preparing formulations for in vivo studies.
|
| Toxicity/Toxicokinetics |
Toxicological data for INVIVO35833 are limited, as it is a research compound. Its primary value is as a tool for studying stem cell biology. While specific toxicity profiles are not detailed, its mechanism of promoting Oct3/4 expression is not expected to cause acute toxicity in standard cell culture conditions. However, comprehensive toxicological studies are not available in the public domain.
|
| References | |
| Additional Infomation |
INVIVO35833 is a research-use-only compound that functions as a potent inducer of the Oct3/4 transcription factor. It is also known as Oct3/4-inducer-1 and has the CAS number 1016535-83-3. This compound is a valuable tool for researchers studying pluripotency, stem cell differentiation, and cellular reprogramming. It is not an approved drug.
|
| Molecular Formula |
C15H12FNO
|
|---|---|
| Molecular Weight |
241.2654
|
| Exact Mass |
241.09
|
| CAS # |
1016535-83-3
|
| PubChem CID |
24690924
|
| Appearance |
Off-white to light brown solid powder
|
| Density |
1.2±0.1 g/cm3
|
| Boiling Point |
391.7±27.0 °C at 760 mmHg
|
| Flash Point |
190.7±23.7 °C
|
| Vapour Pressure |
0.0±0.9 mmHg at 25°C
|
| Index of Refraction |
1.568
|
| LogP |
3.07
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
18
|
| Complexity |
282
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C1=CC(=CC=C1CC#N)OCC2=CC=C(C=C2)F
|
| InChi Key |
PXFUNIXWNXRVEJ-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C15H12FNO/c16-14-5-1-13(2-6-14)11-18-15-7-3-12(4-8-15)9-10-17/h1-8H,9,11H2
|
| Chemical Name |
2-[4-[(4-fluorophenyl)methoxy]phenyl]acetonitrile
|
| Synonyms |
INVIVO 35833 INVIVO-35833 Oct3/4-inducer-1, INVIVO35833
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~414.49 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (10.36 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (10.36 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.1447 mL | 20.7237 mL | 41.4473 mL | |
| 5 mM | 0.8289 mL | 4.1447 mL | 8.2895 mL | |
| 10 mM | 0.4145 mL | 2.0724 mL | 4.1447 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
