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Purity: =98.24%
Indocyanine Green (IC Green; Cardiogreen; Fox) is a non-toxic tricarbocyanine/fluorescent dye used diagnostically in liver function tests and to determine blood volume and cardiac output, as well as for ophthalmic angiography. Indocyanine Green has an Emission (Em) of 813 nm, and an Excitation (Ex) of 785 nm.
| Targets |
Fluorescent dye
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|---|---|
| ln Vitro |
The expression of BAX was dramatically elevated in HGF cells by indocyanine green (Foxgreen)-photodynamic therapy (ICG-PDT) at a concentration of 1000 μg/mL. On the other hand, BCL-2 gene Impact expression was not significantly affected by laser irradiation or ICG.
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| Cell Assay |
Cell Staining Example 1
Indocyanine green is a non-toxic/low toxic fluorescent dye. It can only be taken up by hepatocytes after intravenous injection, after which it is secreted by hepatocytes. When cultured with serum-free medium, hepatocytes take up Indocyanine green, and the cytoplasm appears green. After restoration of serum, Indocyanine green is secreted out of the cytoplasm. Method: For Indocyanine green uptake assay. 1. Add Indocyanine green to serum-free hepatocyte medium with a final concentration of 1 mg/mL and cells are cultured with the medium for 1 hour at 37℃. 2. Remove serum-free medium, and wash cells with PBS for three times. 3. Cells are examined and imaged with a microscope (IX71FL, OLYMPUS). Cell Staining Example 2 Indocyanine green is a non-toxic/low toxic fluorescent dye. It can be taken up by lymph and provides contrast for visualizing the lumen of lymphatic vessels using its single-photon fluorescence in both animal models and human subjects. Method: For noninvasive 2PF imaging. |
| Animal Protocol |
In Vivo Staining Example 1
Indocyanine green is a non/low-toxic fluorescent dye that can be used to detect the distribution of polysaccharide-nanoemulsion following oral administration. Method: For polysaccharide-nanoemulsion distribution detecting. 1. Indocyanine green is dissolved in the polysaccharide aqueous solution and then added to the nanoemulsion to form an complex (The final concentration of Indocyanine green in both solutions is 0.05 mg/mL). 2. A total of 9 BALB/C nude mice are assigned to each of three experimental groups. The mice sre subjected to fasting 4 h following their respective treatment and then placed in a live imaging device (Lumina XR Series III; Perkin-Elmer, Inc) to observe fluorescence distribution in vivo. |
| ADME/Pharmacokinetics |
Absorption, Distribution and Excretion
FOLLOWING IV INJECTION, INDOCYANINE GREEN IS RAPIDLY BOUND TO PLASMA PROTEIN, QUICKLY REMOVED FROM CIRCULATION BY LIVER, & EXCRETED IN BILE IN UNCONJUGATED FORM. WITH INDOCYANINE GREEN, IT HAS BEEN SHOWN THAT RAT HAS HIGHER MAX BILIARY EXCRETION RATE (0.065 MG/KG/MIN) THAN DOES RABBIT (0.05 MG/KG/MIN) OR DOG (0.027 MG/KG/MIN). T/2 INCR WITH INCR DOSAGE. ... CONSIDERABLE DIFFERENCE HAS BEEN NOTED BETWEEN NORMAL MONGRELS & PUREBRED BEAGLES IN CLEARANCE TIMES. APPARENT HEPATIC EXTRACTION OF INDOCYANINE GREEN IN RATS WAS SURPRISINGLY LOW @ DOSES ABOVE 1 MG/KG. CHANGES IN HEPATIC BLOOD FLOW WOULD NOT ALTER CLEARANCE. For more Absorption, Distribution and Excretion (Complete) data for INDOCYANINE GREEN (10 total), please visit the HSDB record page. Metabolism / Metabolites ...INDOCYANINE GREEN...APPEARS IN BILE UNCHANGED |
| Toxicity/Toxicokinetics |
Effects During Pregnancy and Lactation
◉ Summary of Use during Lactation Data from one patient indicates that a low subcutaneous dose of indocyanine green is not detectable in breastmilk. No data are available on the user of larger intravenous doses. Until more data become available, indocyanine green should be used with caution during breastfeeding, especially while nursing a newborn or preterm infant. ◉ Effects in Breastfed Infants Relevant published information was not found as of the revision date. ◉ Effects on Lactation and Breastmilk Relevant published information was not found as of the revision date. Interactions INCR IN HEPATIC EXCRETORY FUNCTION HAS BEEN OBSERVED AFTER PRETREATMENT WITH SOME DRUGS. FOR EXAMPLE, PHENOBARBITAL HAS BEEN DEMONSTRATED TO INCR PLASMA DISAPPEARANCE & BILIARY EXCRETION OF...INDOCYANINE GREEN... SUPPRESSION OF BILIARY SECRETION OF INDOCYANINE GREEN...BY PRETREATMENT WITH SKF 525-A WAS SHOWN TO BE DUE TO TEMP EFFECT RATHER THAN TO EFFECT ON HEPATIC ENDOPLASMIC RETICULUM. INDOCYANINE GREEN DECR INITIAL RATE OF UPTAKE OF OUABAIN IN RAT LIVER CELLS BY MORE THAN 50%. DATA INDICATE THAT CMPD MAY ENTER LIVER CELLS BY PROCESS SIMILAR TO ACTIVE CARRIER-MEDIATED TRANSPORT. IN MALE RATS INJECTED IV WITH 1.0 MG/KG MERCURY, INDOCYANINE GREEN INHIBITED BILIARY EXCRETION OF METHYLMERCURY. For more Interactions (Complete) data for INDOCYANINE GREEN (13 total), please visit the HSDB record page. |
| References | |
| Additional Infomation |
Indocyanine green is a cyanine dye, a benzoindole and a 1,1-diunsubstituted alkanesulfonate.
Indocyanine Green is a nontoxic, fluorescent, tricarbocyanine dye with a peak spectral absorption at 790 nm, with application in determining cardiac output, hepatic function, liver blood flow, as well as ophthalmic angiography. Upon administration, indocyanine green (ICG) rapidly binds to its principle carrier, plasma protein, and is thereby confined to the vascular space. This agent, with a half-life of 150 to 180 seconds, is removed exclusively by the liver from circulation to bile juice. Furthermore, due to poor uptake, ICG is not suitable for angiography or functional output analysis of kidney, lung, cerebro-spinal, or peripheral tissues. A tricarbocyanine dye that is used diagnostically in liver function tests and to determine blood volume and cardiac output. See also: indocyanine green acid form (has active moiety). Therapeutic Uses Dyes TO DETERMINE CARDIAC OUTPUT, HEPATIC FUNCTION, & LIVER BLOOD FLOW. FOR HEPATIC FUNCTION STUDIES, CALCULATED AMT OF DIAGNOSTIC AGENT IS INJECTED INTO ARM VEIN. 20 MIN AFTER INJECTION, 6 ML OF VENOUS BLOOD IS WITHDRAWN FROM OPPOSITE ARM. AFTER COAGULATION & CENTRIFUGATION, CLEAR SERUM IS READ IN PHOTOMETER @ 800-810 NM. DYE RETENTION OF LESS THAN 4% IS FOUND IN HEALTHY SUBJECTS. ... FAILURE TO REMOVE DYE, AS INDICATED BY SERUM LEVELS IN EXCESS OF 4%, IS INDICATIVE OF IMPAIRED HEPATIC FUNCTION. USUAL, IV, BLOOD VOL DETERMINATION, 5 MG IN 1 ML; HEPATIC FUNCTION DETERMINATION, 0-5 MG/KG. DYE IS STABLE IN PLASMA & WHOLE BLOOD PERMITTING LATER ANALYSIS. USERS VARY BOTH CONCN & DOSAGE. IN MAN TOTAL DOSAGE HAS NORMALLY BEEN BELOW 2 MG/KG. Drug Warnings ...CONTAINS SMALL AMT OF /5%/ SODIUM IODIDE; THUS, IT SHOULD BE USED WITH CAUTION IN PT ALLERGIC TO IODIDES & RADIOACTIVE IODINE UPTAKE STUDIES SHOULD NOT BE PERFORMED FOR AT LEAST 1 WK FOLLOWING ITS USE. SINCE PROBENECID HAS BEEN SHOWN IN DOGS TO AFFECT HEPATIC UPTAKE, THIS POSSIBILITY SHOULD BE KEPT IN MIND. SAFE USE OF THIS DRUG IN PREGNANCY HAS NOT BEEN ESTABLISHED. USE FRESH SOLN ONLY AS RECOMMENDED. |
| Molecular Formula |
C43H47N2NAO6S2
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|---|---|
| Molecular Weight |
774.9629
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| Exact Mass |
774.277
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| Elemental Analysis |
C, 66.64; H, 6.11; N, 3.61; Na, 2.97; O, 12.39; S, 8.27
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| CAS # |
3599-32-4
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| PubChem CID |
5282412
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| Appearance |
Light green to dark green solid powder
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| Boiling Point |
157ºC
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| Melting Point |
235 °C
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| LogP |
10.555
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
12
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| Heavy Atom Count |
54
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| Complexity |
1520
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| Defined Atom Stereocenter Count |
0
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| SMILES |
S(C([H])([H])C([H])([H])C([H])([H])C([H])([H])[N+]1C2C([H])=C([H])C3=C([H])C([H])=C([H])C([H])=C3C=2C(C([H])([H])[H])(C([H])([H])[H])C=1/C(/[H])=C(/[H])\C(\[H])=C(/[H])\C(\[H])=C(/[H])\C(\[H])=C1/C(C([H])([H])[H])(C([H])([H])[H])C2C3=C([H])C([H])=C([H])C([H])=C3C([H])=C([H])C=2N/1C([H])([H])C([H])([H])C([H])([H])C([H])([H])S(=O)(=O)[O-])(=O)(=O)[O-].[Na+]
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| InChi Key |
MOFVSTNWEDAEEK-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C43H48N2O6S2.Na/c1-42(2)38(44(28-14-16-30-52(46,47)48)36-26-24-32-18-10-12-20-34(32)40(36)42)22-8-6-5-7-9-23-39-43(3,4)41-35-21-13-11-19-33(35)25-27-37(41)45(39)29-15-17-31-53(49,50)51/h5-13,18-27H,14-17,28-31H2,1-4H3,(H-,46,47,48,49,50,51)/q+1/p-1
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| Chemical Name |
sodium 4-(2-((1E,3E,5E,7E)-7-(1,1-dimethyl-3-(4-sulfonatobutyl)-1,3-dihydro-2H-benzo[e]indol-2-ylidene)hepta-1,3,5-trien-1-yl)-1,1-dimethyl-1H-benzo[e]indol-3-ium-3-yl)butane-1-sulfonate
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| Synonyms |
Cardio-Green Fox IR-125 IR 125 IR125
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~83.33 mg/mL (~107.53 mM)
H2O : ~5 mg/mL (~6.45 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (3.23 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (3.23 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.2904 mL | 6.4519 mL | 12.9039 mL | |
| 5 mM | 0.2581 mL | 1.2904 mL | 2.5808 mL | |
| 10 mM | 0.1290 mL | 0.6452 mL | 1.2904 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
The Use of Indocyanine Green to Visualize Blood Flow to the Gastrojejunostomy During Bariatric Surgery.
CTID: NCT06002906
Phase: Phase 4 Status: Recruiting
Date: 2024-10-26
Products are for research use only; We do not sell to patients
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