My cart
In the shopping cart is not goods, to choose and buy!
  • Product Name
  • Size
  • Quantity
  • Amount
    Selected items : 0 pieces Total : CHECK OUT()
    Indirubin (NSC 105327; Couroupitine B)
    Indirubin (NSC 105327; Couroupitine B)

    Price:
    Market Price:

    This product is for research use only, not for human use. We do not sell to patients.
    Number: - + Pieces(InventoryPieces)
    InvivoChem Cat #: V0225
    CAS #: 479-41-4Purity ≥98%

    Description: Indirubin (also known as NSC-105327; Couroupitine B) is a potent and selective inhibitor of CDK (cyclin-dependent kinases) and GSK-3β (glycogen synthase kinase-3) with potential antitumor activity. It inhibits CDK and  GSK-3β with IC50s of ~5 μM and 0.6 μM, respectively. Indirubin is a nturally occuring and the active ingredient of Danggui Longhui Wan, a mixture of traditional Chinese herbal medicine.

    References: J Biol Chem. 2006 Aug 18;281(33):23425-35; J Biol Chem. 2001 Jan 5;276(1):251-60.

    Customer Validation
    Official Supplier of
    • VE
    • OF
    • YALE
    • hhmi
    • 香港大学
    Related Products
    Publications Citing InvivoChem Products
    • Physicochemical and Storage Information
    • Protocol
    • Quality Control Documentation
    • Related Biological Data
    • Customer Review

    Molecular Weight (MW)

    262.26

    Formula

    C16H10N2O2

    CAS No.

    479-41-4

    Storage

    -20℃ for 3 years in powder form

    -80℃ for 2 years in solvent

    Solubility (In vitro)

    DMSO: 53 mg/mL (202.1 mM)

    Water: <1 mg/mL

    Ethanol: <1 mg/mL

    Other info

    Chemical Name: 3-(1,3-Dihydro-3-oxo-2H-indol-2-ylidene)-1,3-dihydro-2H-indol-2-one

    InChi Key: CRDNMYFJWFXOCH-YPKPFQOOSA-N

    InChi Code: InChI=1S/C16H10N2O2/c19-15-10-6-2-4-8-12(10)17-14(15)13-9-5-1-3-7-11(9)18-16(13)20/h1-8,17H,(H,18,20)/b14-13-

    SMILES Code: O=C1NC2=C(C=CC=C2)/C1=C3NC4=C(C=CC=C4)C/3=O

    Synonyms

    NSC 105327, Couroupitine B; C.I. 73200, Indigo red, Indigopurpurin; NSC-105327, NSC 105327; NSC105327.


    • Molarity Calculator
    • Dilution Calculator
    • The molarity calculator equation

      Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

      • Mass
      • Concentration
      • Volume
      • Molecular Weight *
      • =
      • ×
      • ×
    • The dilution calculator equation

      Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

      This equation is commonly abbreviated as: C1V1 = C2V2

      • Concentration (start)
      • ×
      • Volume (start)
      • =
      • Concentration (final)
      • ×
      • Volume (final)
      • ×
      • =
      • ×
      • C1
      •  
      • V1
      •  
      • C2
      •  
      • V2

    In Vitro

    In vitro activity: Indirubin is the active ingredient of Danggui Longhui Wan, a mixture of plants that is used in traditional Chinese medicine to treat chronic diseases. Indirubin inhibits CDKs activity with IC50 of 2.2 - 10 μM resulting in cell cycle arrest in the G2/M phase. Indirubin also inhibits GSK-3β with an IC50 of 0.6 μM, attenuating CDK5- and GSK-3β-mediated tau phosphorylation, a process over-active in Alzheimer disease states. It also suppresses tumor necrosis factor (TNF)-induced NF-κB activation in a dose- and time-dependent manner. Indirubin also suppresses the NF-κB activation induced by various inflammatory agents and carcinogens. Indirubin blocks the phosphorylation and degradation of IκBα through the inhibition of activation of IκBα kinase and phosphorylation and nuclear translocation of p65.

     

    Cell Assay: Indirubin exerted its inhibitory effects not only on interferon-γ production by human myelomonocytic HBL-38 cells but also on interferon-γ and interleukin-σ production by murine splenocytes with no influence on the proliferation of either cells.

    In Vivo

    Because of Indirubin’s inhibitory activity on interferon-γ production, the authors further investigated the effects of indirubin on 2,4,6-trinitro-l-chlorobenzene-induced delayed-type hypersensitivity. When injected intraperitoneally, indirubin significantly inhibited the ear swelling of TNCB-elicited mice. Moreover,the amount of interferon-γ in the culture supernatants of elicited mouse lymphocytes was inhibited by indirubin treatment 

    Animal model

    Mice

    Formulation & Dosage

    i.p.

    References

    J Biol Chem. 2006 Aug 18;281(33):23425-35; J Biol Chem. 2001 Jan 5;276(1):251-60.


    These protocols are for reference only. InvivoChem does not independently validate these methods.

    Indirubin

    J Biol Chem. 2001 Jan 5;276(1):251-60.

    Indirubin

    Indirubin inhibits TNF-induced expression of NF-κB-dependent antiapoptotic, proliferative, and metastatic proteins.

    Indirubin

    Indirubin enhances TNF-induced cytotoxicity.


    评论

      Home Prev Next Last page / pices

      发评论

      ×
      Your information is safe with us. * Required Fields.
      Products are for research use only;  We do not sell to patients
      Tel: 1-708-310-1919
      Fax: 1-708-557-7486
      Subscribe to our E-newsletter
      • Name*
      • *
      • E-mail*
      • *
      • instructions:
      • *
      Copyright 2020 InvivoChem LLC | All Rights Reserved
      prompt
      Do you confirm the receipt?