| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 100mg | |||
| Other Sizes |
| ln Vitro |
In human fibroblasts, illudin S (0-0.26 nM; 24-72 hours) exhibits cytotoxicity [2].
In vitro, Illudin S is a potent antitumor sesquiterpene. It causes a complete block of cell cycling at the G1-S phase interface, particularly in myeloid and T-lymphocyte leukemia cells with IC50 values of 6-11 nM. It inhibits DNA synthesis and induces apoptosis. |
|---|---|
| ln Vivo |
In vivo, Illudin S has shown activity against various tumor types in preclinical studies. It is a candidate for further development in cancer therapeutics. However, its genotoxicity and potential toxicity limit its clinical application.
|
| Enzyme Assay |
In vitro assays for Illudin S typically measure its effects on DNA synthesis and cell cycle progression. DNA synthesis is measured by the incorporation of radiolabeled thymidine. Cell cycle analysis is performed by flow cytometry. Its cytotoxicity is assessed using various cancer cell lines.
|
| Cell Assay |
Cell viability assay [2]
Cell Types: human fibroblasts Tested Concentrations: 0-0.26 nM Incubation Duration: 24 hrs (hours), 72 hrs (hours) Experimental Results: diminished cell survival rate. Cellular assays for Illudin S are performed using cancer cell lines, particularly leukemia cells. Cells are treated with the compound, and cell viability is assessed using MTT or CellTiter-Glo assays. Apoptosis is measured by Annexin V staining or caspase activity assays. |
| Animal Protocol |
In vivo animal studies with Illudin S are conducted in mouse xenograft models of various cancers. Mice bearing subcutaneous tumors are treated with the compound. Tumor volume is measured over time, and tumor growth inhibition is calculated. Its efficacy and toxicity are evaluated.
|
| ADME/Pharmacokinetics |
Illudin S is a natural product with potent antitumor activity. Its molecular weight is 262.34 g/mol. For research use, it is typically dissolved in DMSO for in vitro studies. Its pharmacokinetic properties have been studied in preclinical models.
|
| Toxicity/Toxicokinetics |
Illudin S is genotoxic and has significant toxicity, which limits its clinical application. It is cytotoxic and can cause DNA damage.
|
| References | |
| Additional Infomation |
Illudin S is a sesquiterpene compound. It has been reported in Omphalotus olearius, Omphalotus guepiniformis, and other organisms with available data.
Illudin S is a natural sesquiterpene with potent antitumor activity. It inhibits DNA synthesis and causes cell cycle arrest. However, its genotoxicity and toxicity have limited its development as a therapeutic agent. Illudin S is used as a research tool to study DNA damage and cell cycle regulation. |
| Molecular Formula |
C15H21O4
|
|---|---|
| Molecular Weight |
265.32
|
| Exact Mass |
264.136
|
| Elemental Analysis |
C, 68.16; H, 7.63; O, 24.21
|
| CAS # |
1149-99-1
|
| PubChem CID |
344200
|
| Appearance |
Off-white to yellow solid powder
|
| Density |
1.34g/cm3
|
| Boiling Point |
509.6ºC at 760mmHg
|
| Flash Point |
276.1ºC
|
| Vapour Pressure |
1.61E-12mmHg at 25°C
|
| Index of Refraction |
1.619
|
| LogP |
0.716
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
1
|
| Heavy Atom Count |
19
|
| Complexity |
540
|
| Defined Atom Stereocenter Count |
3
|
| SMILES |
CC1=C2C(=C[C@@](C)(CO)[C@@H]2O)C(=O)[C@@](C)(C31CC3)O
|
| InChi Key |
DDLLIYKVDWPHJI-RDBSUJKOSA-N
|
| InChi Code |
InChI=1S/C15H20O4/c1-8-10-9(6-13(2,7-16)12(10)18)11(17)14(3,19)15(8)4-5-15/h6,12,16,18-19H,4-5,7H2,1-3H3/t12-,13+,14+/m1/s1
|
| Chemical Name |
(1R,2S,5R)-1,5-dihydroxy-2-(hydroxymethyl)-2,5,7-trimethylspiro[1H-indene-6,1'-cyclopropane]-4-one
|
| Synonyms |
DR 15978; DR-15978; Illudin S
|
| HS Tariff Code |
2934.99.03.00
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~189.16 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (9.46 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.46 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.7690 mL | 18.8452 mL | 37.6903 mL | |
| 5 mM | 0.7538 mL | 3.7690 mL | 7.5381 mL | |
| 10 mM | 0.3769 mL | 1.8845 mL | 3.7690 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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