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| 5mg |
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| 25mg |
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Purity: ≥98%
ID-8 is a novel and potent small molecule DYRK (Dual-specificity tyrosine phosphorylation-regulated kinase) inhibitor that sustains embryonic stem cell self-renewal in long-term culture. ID-8 (0.5 μM) increased hESC survival by 1.1% in HES2 cells. Wnt3a and ID-8 together totally blocked Wnt-induced morphological differentiation and raised survival by 1.7%. Additionally, ID-8 dramatically decreased the expression of Wnt-induced differentiation marker genes GATA6, GSC, SOX17, and CDX2. By inhibiting DYRKs, ID-8 promoted Wnt-mediated hESC proliferation and survival in hESCs. Additionally, ID-8 directly targets the DYRK family.
| Targets |
DYRK
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| ln Vivo |
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| Cell Assay |
The hESCs in feeder-free culture are, in short, fully dissociated using 0.05% trypsin-EDTA, seeded at 104 cells per well in Matrigel-coated 6-well culture plates, and cultured in MEF-CM. When seeding begins and continues throughout the entire culturing process, different concentrations of Wnt3, IQ-1, ID-8, and/or ICG-001 are added to the culture media. All assays involve a microscope examination of the cell and colony morphology, and a count of the number of colonies after 7 days of culture is used to determine the replating efficiency.
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| Animal Protocol |
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| References |
| Molecular Formula |
C16H14N2O4
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| Molecular Weight |
298.29
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| Exact Mass |
298.10
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| Elemental Analysis |
C, 64.42; H, 4.73; N, 9.39; O, 21.45
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| CAS # |
147591-46-6
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| Related CAS # |
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| Appearance |
Solid powder
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| SMILES |
CC1=C(C2=C(N1C3=CC=C(C=C3)OC)C=C(C=C2)O)[N+](=O)[O-]
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| InChi Key |
VVZNWYXIOADGSW-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C16H14N2O4/c1-10-16(18(20)21)14-8-5-12(19)9-15(14)17(10)11-3-6-13(22-2)7-4-11/h3-9,19H,1-2H3
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| Chemical Name |
1-(4-methoxyphenyl)-2-methyl-3-nitroindol-6-ol
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| Synonyms |
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (8.38 mM) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3524 mL | 16.7622 mL | 33.5244 mL | |
| 5 mM | 0.6705 mL | 3.3524 mL | 6.7049 mL | |
| 10 mM | 0.3352 mL | 1.6762 mL | 3.3524 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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