| Size | Price | Stock | Qty |
|---|---|---|---|
| 10mg |
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| 50mg |
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| Other Sizes |
| Targets |
ICG-carboxylic acid does not have a traditional biological target; it is a fluorescent probe used for imaging applications. It is a near-infrared (NIR) cyanine dye that can be used for fluorescence-guided surgery, blood flow measurement, angiography, and targeted imaging. The carboxylic acid group allows conjugation to antibodies or peptides for targeted applications.
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|---|---|
| ln Vitro |
Because of its well-established fluorescent qualities, indocyanine green (ICG), a water-soluble chemical, is often and safely employed in medical diagnostics. It has been applied to the identification of important structures, such as intra-abdominal malignancies, during fluorescence-guided surgery [1].
In vitro, ICG-carboxylic acid is used as a fluorescent probe for labeling and imaging cells or tissues. It can be conjugated to targeting molecules for specific cell labeling. Its well-established fluorescent qualities make it a safe and commonly used reagent in medical diagnostics. |
| ln Vivo |
In vivo, ICG is used for fluorescence-guided surgery to identify critical structures, including intra-abdominal tumors. ICG-carboxylic acid, as a derivative, can be used for similar applications, including targeted imaging. It can absorb near-infrared laser energy and release heat within the dyed tissue, which has potential for photothermal therapy.
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| Enzyme Assay |
In vitro assays for ICG-carboxylic acid involve measuring its fluorescence properties. The compound's excitation and emission spectra are recorded to confirm its NIR fluorescence characteristics. Its ability to label cells or proteins is assessed by fluorescence microscopy or flow cytometry.
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| Cell Assay |
Cellular assays for ICG-carboxylic acid are performed using cells incubated with the compound or its conjugates. Cellular uptake and localization are assessed by fluorescence microscopy. The compound's fluorescence intensity is measured to quantify labeling efficiency.
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| Animal Protocol |
In vivo animal studies for ICG-carboxylic acid are not detailed in the available sources. ICG is widely used in preclinical and clinical imaging studies. ICG-carboxylic acid can be used for similar applications, including fluorescence-guided surgery and tumor imaging.
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| ADME/Pharmacokinetics |
ICG-carboxylic acid is a fluorescent probe with absorption peaking at 800 nm. It is water-soluble and has a well-established safety profile in medical diagnostics. Specific PK parameters are not detailed in the available sources.
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| Toxicity/Toxicokinetics |
ICG has a well-established safety profile and is commonly used in clinical diagnostics. ICG-carboxylic acid is expected to have a similar safety profile. Specific toxicological data are not provided in the available sources.
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| References | |
| Additional Infomation |
ICG-carboxylic acid is a near-infrared fluorescent probe derived from indocyanine green. It is used for fluorescence-guided surgery, angiography, and targeted imaging. The compound is available from commercial suppliers for research purposes.
|
| Molecular Formula |
C45H50N2O5S
|
|---|---|
| Molecular Weight |
730.953911304474
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| Exact Mass |
730.344
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| CAS # |
181934-09-8
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| PubChem CID |
145710953
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| Appearance |
Brown to black solid powder
|
| LogP |
9.4
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| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
14
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| Heavy Atom Count |
53
|
| Complexity |
1530
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| Defined Atom Stereocenter Count |
0
|
| SMILES |
S(CCCCN1C2C=CC3C=CC=CC=3C=2C(C)(C)/C/1=C/C=C/C=C/C=C/C1C(C)(C)C2C3C=CC=CC=3C=CC=2[N+]=1CCCCCC(=O)O)(=O)(=O)[O-]
|
| InChi Key |
HIBVKROMGIHYKL-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C45H50N2O5S/c1-44(2)39(46(30-16-8-11-25-41(48)49)37-28-26-33-19-12-14-21-35(33)42(37)44)23-9-6-5-7-10-24-40-45(3,4)43-36-22-15-13-20-34(36)27-29-38(43)47(40)31-17-18-32-53(50,51)52/h5-7,9-10,12-15,19-24,26-29H,8,11,16-18,25,30-32H2,1-4H3,(H-,48,49,50,51,52)
|
| Chemical Name |
4-[(2Z)-2-[(2E,4E,6E)-7-[3-(5-carboxypentyl)-1,1-dimethylbenzo[e]indol-3-ium-2-yl]hepta-2,4,6-trienylidene]-1,1-dimethylbenzo[e]indol-3-yl]butane-1-sulfonate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~68.40 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (3.42 mM) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.3681 mL | 6.8404 mL | 13.6808 mL | |
| 5 mM | 0.2736 mL | 1.3681 mL | 2.7362 mL | |
| 10 mM | 0.1368 mL | 0.6840 mL | 1.3681 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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