| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
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| ln Vitro |
ICCB-19 hydrochloride has an IC50 of roughly 1 μM and inhibits both RIPK1-dependent cellular fluorescence (RDA) and cellular fluorescence caused by benezomib [1]. mTOR is not affected by ICCB-19 hydrochloride. Using ICCB-19 hydrochloride (10 μM) as a therapy, autophagy is stimulated via the following mechanisms. The number of DsRed-FYVE spots, levels, and inhibitory inhibitory activity of VPS34 can be increased by cells via the E3 ubiquitin that links cIAP1 and cIAP2 and the adaptor TRAF2[1]. The fast activation of RIPK1 in complex I caused by TNF is lessened by ICCB-19 hydrochloride (10 μM). TRADD, HOIP, and A20 recruitment are increased upon treatment with ICCB-19 hydrochloride, however beclin 1 K63 ubiquitination is not affected [1]. RIPK1 recruitment to complex I is increased[1].
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| ln Vivo |
Tradd is decreased by ICCB-19 hydrochloride?*? The TNF-induced factor target gene products NOS and COXII27, as well as the factor cytokine expression in cells stimulated by related molecular patterns, can all be downregulated by ICCB-19 hydrochloride [1].
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| References |
[1]. Daichao Xu, et al. Modulating TRADD to restore cellular homeostasis and inhibit apoptosis. Nature. 2020 Nov;587(7832):133-138.
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| Molecular Formula |
C12H22CLN3OS
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|---|---|
| Molecular Weight |
291.84
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| CAS # |
1803605-68-6
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| SMILES |
Cl.C1(NC(=O)CSC2NCCN=2)CCCCCC1
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| Synonyms |
ICCB19 HClICCB 19 HClICCB-19 HClICCB-19hydrochlorideICCB 19hydrochlorideICCB19hydrochloride
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ≥ 100 mg/mL (~342.65 mM)
DMSO : ~83.33 mg/mL (~285.53 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (7.13 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (7.13 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (7.13 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.4265 mL | 17.1327 mL | 34.2654 mL | |
| 5 mM | 0.6853 mL | 3.4265 mL | 6.8531 mL | |
| 10 mM | 0.3427 mL | 1.7133 mL | 3.4265 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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