| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Targets |
IA-alkyne targets cysteine residues in proteins through its iodoacetamide group, which alkylates thiol groups to form a stable thioether bond. This covalent modification allows for the labeling and identification of cysteine-containing proteins in complex mixtures. The alkyne group serves as a bioorthogonal handle for click chemistry, enabling the conjugation of a detection tag (e.g., biotin-azide or fluorescent azide) to the labeled proteins. IA-alkyne is also a TRP channel (TRPC) agonist. By targeting cysteine residues and acting as a TRPC agonist, IA-alkyne is a versatile tool for studying protein function and signaling pathways.
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| ln Vitro |
Cysteine and IA-Alkyne (2.5–10 μM; 1 hour) react. IA-Alkyne was used to treat the soluble fraction of HeLa internal cells (2.0 mg/mL). Conjugation of CuAAC to hydrophobic tags of fluorescent rhodamine-labeled proteome samples was used for analysis, followed by SDS-PAGE folding. The highest fluorescence intensity is found in IA-Alkyne, according to fluorescence imaging inside the hydrophobicity [2].
In vitro, IA-alkyne is used for quantitative cysteine-reactivity profiling, where it enables the identification and quantification of cysteine residues in proteins. It is a click chemistry reagent that can undergo CuAAc with azide-containing molecules. As a TRP channel agonist, it has the potential for the study of respiratory infections. Its activity is typically measured using proteomics-based approaches, where the labeled proteins are identified and quantified by mass spectrometry. These in vitro studies confirm IA-alkyne's utility as a cysteine-labeling probe and TRPC agonist. |
| ln Vivo |
In vivo activity data for IA-alkyne is limited, as the compound is primarily used as a research tool in in vitro studies. As a TRP channel agonist, it has the potential for the study of respiratory infections. However, specific in vivo protocols and results are not detailed in standard product descriptions. IA-alkyne is a research compound used for cysteine-reactivity profiling and the study of TRP channel function.
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| Enzyme Assay |
In vitro assays for IA-alkyne measure its ability to label cysteine residues in proteins and its activity as a TRPC agonist. Cysteine-labeling assays involve incubating proteins or cell lysates with IA-alkyne, followed by click chemistry to attach a detection tag. The labeled proteins are then identified and quantified by mass spectrometry or gel electrophoresis. TRPC agonist activity can be assessed using calcium flux assays in cells expressing TRPC channels. These assays confirm the compound's utility as a cysteine-labeling probe and TRPC agonist.
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| Cell Assay |
In vitro cell-based assays for IA-alkyne are used to study its effects on TRPC channel function and to profile cysteine reactivity in cells. Cells expressing TRPC channels are treated with IA-alkyne, and calcium flux is measured using a fluorescent calcium indicator. For cysteine-reactivity profiling, cells are treated with IA-alkyne, and the labeled proteins are identified and quantified by mass spectrometry. These assays confirm the compound's activity as a TRPC agonist and its utility as a cysteine-labeling probe.
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| Animal Protocol |
In vivo animal experiments for IA-alkyne are not extensively described in the available literature. As a research compound, its use in vivo would be determined by the specific research question being addressed. A typical protocol for studying a TRPC agonist would involve its administration to animal models of respiratory infections or other diseases. However, specific protocols for IA-alkyne are not detailed.
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| ADME/Pharmacokinetics |
IA-alkyne has a molecular weight of 265.09 g/mol and a molecular formula of C8H12INO. Its IUPAC name is N-Hex-5-ynyl-2-iodo-acetamide. It is a solid compound with a purity of >98.0% (GC). For storage, it is recommended to keep the compound at -20°C. Detailed pharmacokinetic properties such as absorption, distribution, metabolism, and excretion (ADME) have not been extensively characterized. As a research compound, its stability is maintained by proper storage as a dry powder.
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| Toxicity/Toxicokinetics |
Detailed toxicity data for IA-alkyne is not provided in standard product descriptions. As a research compound, its toxicity profile has not been extensively characterized. IA-alkyne contains an iodoacetamide group, which is a reactive alkylating agent and can be toxic. It should be handled with caution using appropriate personal protective equipment. Its use is limited to research applications and it is not intended for human or veterinary use.
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| References | |
| Additional Infomation |
IA-alkyne is a research compound and is not approved for any clinical or therapeutic use. It is a trifunctional probe that combines an iodoacetamide group for covalent labeling of cysteine residues, an alkyne group for click chemistry, and a linker. IA-alkyne is a TRP channel (TRPC) agonist and has the potential for the study of respiratory infections. It is also used for quantitative cysteine-reactivity profiling, where it enables the identification and quantification of cysteine residues in proteins. IA-alkyne is a click chemistry reagent that can undergo CuAAc with azide-containing molecules. It is a valuable tool for studying protein function, signaling pathways, and cysteine reactivity.
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| Molecular Formula |
C8H12INO
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|---|---|
| Molecular Weight |
265.0945
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| Exact Mass |
264.996
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| CAS # |
930800-38-7
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| Related CAS # |
124-87-8;
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| PubChem CID |
44448710
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| Appearance |
White to off-white solid powder
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| LogP |
2.181
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
1
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
11
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| Complexity |
159
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C#CCCCCNC(=O)CI
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| InChi Key |
YCRUVTMZPHEOAM-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C8H12INO/c1-2-3-4-5-6-10-8(11)7-9/h1H,3-7H2,(H,10,11)
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| Chemical Name |
N-hex-5-ynyl-2-iodoacetamide
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| Synonyms |
IA alkyne IAalkyne IA-alkyne
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~377.23 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (9.43 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.43 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (9.43 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.7723 mL | 18.8615 mL | 37.7230 mL | |
| 5 mM | 0.7545 mL | 3.7723 mL | 7.5446 mL | |
| 10 mM | 0.3772 mL | 1.8862 mL | 3.7723 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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