| Size | Price | Stock | Qty |
|---|---|---|---|
| 10mg |
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| 50mg |
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| Other Sizes |
|
| ln Vitro |
To PANC-1 cells in culture medium, I-OMe-Tyrphostin AG 538 (I-OMe-AG 538) (0.1-1000 μM; 24 hours) is cytotoxic [1].
|
|---|---|
| Cell Assay |
Cell Viability Assay[1]
Cell Types: PANC-1 cells Tested Concentrations: 0.1, 1, 10, 1000 µM Incubation Time (0-3 µM; 1 hour) Inhibits the phosphorylation of IGF-1R, Akt and Erk[1]. Incubation Duration: 24 hour Experimental Results: Cytotoxic to PANC-1 cells in nutrient-poor medium. Western Blot Analysis [1] Cell Types: PANC-1 cells (stimulated with 50 ng/ml IGF-1 for 10 minutes) Tested Concentrations: 0.03, 0.3, 3 µM Incubation Duration: 1 hour Experimental Results: Phosphorylation of IGF-1R, Akt was Block and Elk. |
| References |
|
| Additional Infomation |
2-[(3,4-dihydroxyphenyl)-oxomethyl]-3-(4-hydroxy-3-iodo-5-methoxyphenyl)-2-propenenitrile is a member of chalcones.
|
| Molecular Formula |
C17H12INO5
|
|---|---|
| Molecular Weight |
437.185357093811
|
| Exact Mass |
436.976
|
| CAS # |
1094048-77-7
|
| PubChem CID |
5353685
|
| Appearance |
Yellow to orange solid powder
|
| LogP |
3.2
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
6
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
24
|
| Complexity |
542
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
IC1C(=C(C=C(C=1)C=C(C#N)C(C1C=CC(=C(C=1)O)O)=O)OC)O
|
| InChi Key |
HSRMHXWCTRFVHK-NYYWCZLTSA-N
|
| InChi Code |
InChI=1S/C17H12INO5/c1-24-15-6-9(5-12(18)17(15)23)4-11(8-19)16(22)10-2-3-13(20)14(21)7-10/h2-7,20-21,23H,1H3/b11-4+
|
| Chemical Name |
(E)-2-(3,4-dihydroxybenzoyl)-3-(4-hydroxy-3-iodo-5-methoxyphenyl)prop-2-enenitrile
|
| Synonyms |
IOMeTyrphostin AG 538; I OMe Tyrphostin AG 538
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~114.37 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.72 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.72 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2873 mL | 11.4367 mL | 22.8734 mL | |
| 5 mM | 0.4575 mL | 2.2873 mL | 4.5747 mL | |
| 10 mM | 0.2287 mL | 1.1437 mL | 2.2873 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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