| Size | Price | Stock | Qty |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| ln Vitro |
Hydroxyphenylacetylglycine (4-Hydroxyphenacyglycine) is an endogenous metabolite of humans and an acylglycine [1].
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|---|---|
| References | |
| Additional Infomation |
4-Hydroxyphenylacetylglycine is formed by hydroxylation of phenylacetylglycine at the C-4 position of the benzene ring. It is a mouse metabolite. It is a monocarboxylic acid amide, monocarboxylic acid, and N-acylglycine. It is functionally related to phenylacetylglycine.
There are reports on the presence of 4-hydroxyphenylacetylglycine in Arabidopsis thaliana and honeybee, and relevant data are available. Hydroxyphenylacetylglycine is an acylglycine compound found in human urine [1]. The selected literature [1] focuses on developing an isotope-labeled ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS) method for the quantitative analysis of this compound, rather than studying its pharmacological activity, metabolism, or toxicity [1]. The detection process includes: 1) urine sample pretreatment (acetonitrile precipitation of proteins, solid phase extraction purification); 2) derivatization using isotope-labeled reagents to improve detection sensitivity; 3) ultra-high performance liquid chromatography separation (C18 column, mobile phase: acetonitrile-water solution containing formic acid); 4) mass spectrometry quantification (electrospray ionization, multiple reaction monitoring mode) [1]. This method showed good linearity (R² > 0.995), precision (relative standard deviation < 8%) and recovery (85–105%) for the quantitative analysis of hydroxyphenylacetylglycine in human urine [1]. |
| Molecular Formula |
C10H11NO4
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|---|---|
| Molecular Weight |
209.19864
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| Exact Mass |
209.069
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| CAS # |
28116-23-6
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| Related CAS # |
Hydroxyphenylacetylglycine; 28116-23-6
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| PubChem CID |
440732
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| Appearance |
White to off-white solid powder
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| Density |
1.356 g/cm3
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| Boiling Point |
563ºC at 760 mmHg
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| Flash Point |
294.3ºC
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| Source |
Endogenously produced metabolite
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| LogP |
0.975
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
15
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| Complexity |
234
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=C(O)CNC(CC1=CC=C(O)C=C1)=O
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| InChi Key |
CPPDWYIPKSSNNM-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C10H11NO4/c12-8-3-1-7(2-4-8)5-9(13)11-6-10(14)15/h1-4,12H,5-6H2,(H,11,13)(H,14,15)
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| Chemical Name |
2-[[2-(4-hydroxyphenyl)acetyl]amino]acetic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~100 mg/mL (~478.0 mM; with ultrasonication)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (11.95 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (11.95 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.7801 mL | 23.9006 mL | 47.8011 mL | |
| 5 mM | 0.9560 mL | 4.7801 mL | 9.5602 mL | |
| 10 mM | 0.4780 mL | 2.3901 mL | 4.7801 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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