| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
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| ln Vitro |
Preparation of Hoechst working solution 1.1 Preparation of stock solution Use DMSO to prepare 1 mg/mL Hoechst stock solution. Note: Hoechst storage solution is recommended to be stored at -4℃ or -20℃ in the dark after filling. 1.2 Replacement of working solution: Inject excellent serum-free cell culture medium or PBS into the storage solution before use. The ultimate concentration is 10 μg/mL Hoechst working solution. Note: Please adjust the concentration of Hoechst working fluid according to the real condition, and make it suitable for usage. 2. Suspended cell staining (2.1): Centrifuge cells, add PBS, then wash twice for five minutes each time. When the cell density reaches 1×106/mL 2.2, add 1 mL Hoechst working solution for 3-10 minutes. 2.3 Centrifuge at 400 g for 3-4 minutes and discard. 2.4 Wash the cells twice with PBS, giving them five minutes each time. 2.5 Resuspend the cells in 1 mL of serum-free or PBS, and observe using a fluorescence microscope or flow cytometer. 3. Cell staining (adhesion) 3.1 Culture adherent cells on sterile coverslips. 3.2 Remove the coverslip from the culture medium and aspirate surplus cells. 3.3 Add 100 μL dye working solution, shake gently to completely cover the cells, leaving 3 3.4 Aspirate the dye working solution, wash 2-3 times with culture media, 5 minutes each time, and observe using a fluorescence microscope or flow cytometer. Notes 1. Please adjust the concentration of Hoechst working fluid according to the real conditions. -10 minutes. 2. This product is solely for scientific research by professionals and may not be used for clinical diagnosis or treatment, and may not be used in food or medicine. 3. For your safety and health, please use a lab coat and gloves when operating.
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| References |
| Molecular Formula |
C25H25N7O2S.3[HCL]
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|---|---|
| Molecular Weight |
596.95952
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| Exact Mass |
595.109
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| CAS # |
74681-68-8
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| PubChem CID |
123837
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| Appearance |
Light yellow to green yellow solid powder
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| Melting Point |
>300℃
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| Hydrogen Bond Donor Count |
6
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
38
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| Complexity |
837
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
NZVGXJAQIQJIOY-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C25H25N7O2S.3ClH/c1-31-10-12-32(13-11-31)18-5-9-21-23(15-18)30-25(28-21)17-4-8-20-22(14-17)29-24(27-20)16-2-6-19(7-3-16)35(26,33)34;;;/h2-9,14-15H,10-13H2,1H3,(H,27,29)(H,28,30)(H2,26,33,34);3*1H
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| Chemical Name |
4-[6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazol-2-yl]benzenesulfonamide;trihydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ≥ 6 mg/mL (~10.05 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: 5 mg/mL (8.38 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication (<60°C).
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.6752 mL | 8.3758 mL | 16.7515 mL | |
| 5 mM | 0.3350 mL | 1.6752 mL | 3.3503 mL | |
| 10 mM | 0.1675 mL | 0.8376 mL | 1.6752 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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