| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| 100mg | |||
| Other Sizes |
| Targets |
DNA (minor groove, A/T-rich regions).
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| ln Vitro |
Preparation of Hoechst working solution 1.1 Preparation of stock solution Use DMSO to prepare 1 mg/mL Hoechst stock solution. Note: Hoechst storage solution is recommended to be stored at -4℃ or -20℃ in the dark after filling. 1.2 Replacement of working solution: Inject excellent serum-free cell culture medium or PBS into the storage solution before use. The ultimate concentration is 10 μg/mL Hoechst working solution. Note: Please adjust the concentration of Hoechst working fluid according to the real condition, and make it suitable for usage. 2. Suspended cell staining (2.1): Centrifuge cells, add PBS, then wash twice for five minutes each time. When the cell density reaches 1×106/mL 2.2, add 1 mL Hoechst working solution for 3-10 minutes. 2.3 Centrifuge at 400 g for 3-4 minutes and discard. 2.4 Wash the cells twice with PBS, giving them five minutes each time. 2.5 Resuspend the cells in 1 mL of serum-free or PBS, and observe using a fluorescence microscope or flow cytometer. 3. Cell staining (adhesion) 3.1 Culture adherent cells on sterile coverslips. 3.2 Remove the coverslip from the culture medium and aspirate surplus cells. 3.3 Add 100 μL dye working solution, shake gently to completely cover the cells, leaving 3 3.4 Aspirate the dye working solution, wash 2-3 times with culture media, 5 minutes each time, and observe using a fluorescence microscope or flow cytometer. Notes 1. Please adjust the concentration of Hoechst working fluid according to the real conditions. -10 minutes. 2. This product is solely for scientific research by professionals and may not be used for clinical diagnosis or treatment, and may not be used in food or medicine. 3. For your safety and health, please use a lab coat and gloves when operating.
In vitro, Hoechst S 769121 binds to DNA with enhanced fluorescence intensity upon binding to A/T-rich double-stranded DNA. It is used as a live nuclear marker dye for staining nuclei in live or fixed cells. The dye can be used for living cell labeling and is suitable for multiplexing experiments due to its distinct excitation/emission profile. |
| ln Vivo |
Hoechst S 769121 is not used as a therapeutic agent in vivo; it is a laboratory reagent for cell staining. No in vivo activity data are reported as the compound is used exclusively as a fluorescent stain in cell biology research.
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| Enzyme Assay |
DNA binding assays are performed by incubating Hoechst S 769121 with purified DNA (e.g., calf thymus DNA, plasmid DNA, or synthetic oligonucleotides) in appropriate buffer (10 mM Tris-HCl, pH 7.4, 100 mM NaCl, 1 mM EDTA) at room temperature. Fluorescence intensity is measured using a fluorometer or fluorescence plate reader with excitation at 335 nm and emission at 495 nm. Binding affinity is assessed by titrating increasing concentrations of DNA with a fixed concentration of the dye, and the fluorescence increase is plotted against DNA concentration. Competitive binding studies can be performed using unlabeled DNA-binding compounds. For sequence specificity analysis, A/T-rich and G/C-rich oligonucleotides are used to demonstrate preferential binding to A/T-rich sequences.
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| Cell Assay |
Cells (e.g., adherent or suspension cell lines) are cultured in appropriate medium. For live cell staining, cells are incubated with Hoechst S 769121 at concentrations of 0.5-5 microg/mL in culture medium or PBS for 15-30 minutes at 37degC. Cells are then washed and visualized by fluorescence microscopy (excitation 335 nm, emission 495 nm) or analyzed by flow cytometry. For fixed cell staining, cells are fixed with paraformaldehyde or methanol, permeabilized if necessary, and stained with the dye. The dye is also compatible with multiplexing experiments using other fluorescent probes. Optimal staining conditions should be determined empirically for each cell type.
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| Animal Protocol |
Hoechst S 769121 is a research reagent and is not administered to animals for therapeutic purposes. For ex vivo tissue staining, tissue sections are incubated with the dye (0.5-5 microg/mL) in PBS for 15-30 minutes at room temperature, washed, and mounted for fluorescence microscopy. For in vivo imaging studies, the dye may be administered locally or systemically in animal models to label nuclei, but such applications are limited. No standardized in vivo protocols are established.
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| ADME/Pharmacokinetics |
Hoechst S 769121 (Nuclear Yellow) has molecular formula C2₅H2₈Cl3N₇O2S and molecular weight 596.96. The dye is cell-permeant and can stain live cells without requiring fixation. It is soluble in DMSO and other appropriate solvents. The compound is stable when stored protected from light. Upon binding to DNA, the dye shows significant fluorescence enhancement, with A/T-rich sequences producing the strongest signal.
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| Toxicity/Toxicokinetics |
The dye is classified as a laboratory reagent and should be handled with standard safety precautions. No specific toxicity data are reported for Hoechst S 769121. As a DNA-binding dye, it may have genotoxic potential and should be handled as a potential mutagen. Appropriate personal protective equipment should be used. The compound is for research use only and not for human therapeutic or diagnostic use.
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| References | |
| Additional Infomation |
Hoechst S 769121 (Nuclear Yellow) is a DNA-selective fluorescent dye used extensively in cell biology research. It is part of the Hoechst series of dyes, which are among the most commonly used nuclear stains for fluorescence microscopy and flow cytometry. The dye preferentially binds to A/T-rich regions in the minor groove of double-stranded DNA, with excitation/emission maxima ~335/495 nm when bound. Nuclear Yellow is used for labeling DNA content in live and fixed cells, and its fluorescence can be measured using fluorescence microscopy, microplate fluorometry, or flow cytometry. The compound is available as a research-grade dye and is not an approved diagnostic or therapeutic agent.
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| Molecular Formula |
C25H25N7O2S.3[HCL]
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|---|---|
| Molecular Weight |
596.95952
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| Exact Mass |
595.109
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| CAS # |
74681-68-8
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| PubChem CID |
123837
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| Appearance |
Light yellow to green yellow solid powder
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| Melting Point |
>300℃
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| Hydrogen Bond Donor Count |
6
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
38
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| Complexity |
837
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
NZVGXJAQIQJIOY-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C25H25N7O2S.3ClH/c1-31-10-12-32(13-11-31)18-5-9-21-23(15-18)30-25(28-21)17-4-8-20-22(14-17)29-24(27-20)16-2-6-19(7-3-16)35(26,33)34;;;/h2-9,14-15H,10-13H2,1H3,(H,27,29)(H,28,30)(H2,26,33,34);3*1H
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| Chemical Name |
4-[6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazol-2-yl]benzenesulfonamide;trihydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ≥ 6 mg/mL (~10.05 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 5 mg/mL (8.38 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication (<60°C).
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.6752 mL | 8.3758 mL | 16.7515 mL | |
| 5 mM | 0.3350 mL | 1.6752 mL | 3.3503 mL | |
| 10 mM | 0.1675 mL | 0.8376 mL | 1.6752 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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