| Size | Price | Stock | Qty |
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| 2mg |
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| 5mg |
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| 10mg |
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| ln Vitro |
HIF-1α-IN-2 (compound 7f) reduces MDA-MB-231 and MiaPaCa-2 cell survival at 5 μM and 72 hours, respectively, with IC50 values of 28 nM and 15 nM [1]. With a dose-dependent impact, HIF-1α-IN-2 (0.25, 0.5, and 1 µM; 16–24h) can considerably (0–1 µM; 72h) decrease the expression of HIF-1α and VEGF [1]. inhibited the migration of MDA-MD-231 cells at concentrations of 56%, 0.25 µM, 83%, 0.5 µM, and 85%, 1 µM. It was also detected in MiaPaCa-2 cells. This indicates that HIF-1α- HIF-1α-IN-2 (0-1 μM; 72h) suppresses the levels of HIF-1α mRNA in MDA-MB-231 cells when they are hypoxic [1]. The anti-migration action of IN-2 is distinct [1]. viability test
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| Cell Assay |
Viability assay
Cell Types: MDA-MB-231 cells, MiaPaCa-2 cells [1] Tested Concentrations: 5 μM Incubation Duration: 72 hrs (hours) Experimental Results: Inhibited the viability of these two cell lines, the IC50 in MDA-MB was 28 nM respectively and 15 nM for -231 and MiaPaCa-2 cells, respectively. Cell viability assay Cell Types: MDA-MB-231 cells, MiaPaCa-2 cells [1] Tested Concentrations: 5 μM Incubation Duration: 72 hrs (hours) Experimental Results: Inhibited the viability of these two cell lines, the IC50 in MDA- was 28 nM and 15 nM for MB-231 and MiaPaCa-2 cells respectively. |
| References |
| Molecular Formula |
C21H19N3OS
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|---|---|
| Molecular Weight |
361.46
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| Exact Mass |
361.124
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| CAS # |
2762315-06-8
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| PubChem CID |
163196195
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| Appearance |
Off-white to light yellow solid powder
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| LogP |
5.2
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
26
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| Complexity |
465
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| Defined Atom Stereocenter Count |
0
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| SMILES |
N1C2C(=CC=C(OC)C=2)C(NC2=CC=CC(C3=CSC(C)=N3)=C2)=CC=1C
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| InChi Key |
ILYYBJSTMKDZND-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C21H19N3OS/c1-13-9-19(18-8-7-17(25-3)11-20(18)22-13)24-16-6-4-5-15(10-16)21-12-26-14(2)23-21/h4-12H,1-3H3,(H,22,24)
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| Chemical Name |
7-methoxy-2-methyl-N-[3-(2-methyl-1,3-thiazol-4-yl)phenyl]quinolin-4-amine
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~25 mg/mL (~69.16 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.92 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.92 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (6.92 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7666 mL | 13.8328 mL | 27.6656 mL | |
| 5 mM | 0.5533 mL | 2.7666 mL | 5.5331 mL | |
| 10 mM | 0.2767 mL | 1.3833 mL | 2.7666 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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