| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 100mg | |||
| Other Sizes |
| Targets |
Hexa-His targets metal ions, particularly nickel (Ni²⁺) and cobalt (Co²⁺), through coordination with the imidazole side chains of histidine residues. It binds to immobilized metal affinity chromatography (IMAC) resins, enabling purification of His-tagged recombinant proteins.
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|---|---|
| ln Vitro |
Hexa-His functions as a metal binding site for recombinant proteins, enabling their purification via IMAC. It can be placed on the N- or C-terminus of target proteins using expression vectors. It competitively binds to anti-His antibodies, allowing elution of 6xHis fusion proteins during immunoprecipitation.
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| ln Vivo |
As a peptide tag, Hexa-His is not evaluated for in vivo activity in animal models. Its application is in recombinant protein production and purification systems.
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| Enzyme Assay |
In vitro non-cell assays for Hexa-His involve immobilized metal affinity chromatography (IMAC) where the peptide is used as a fusion tag. The His-tagged protein is loaded onto a column containing Ni²⁺-NTA or Co²⁺-TALON resin, washed to remove contaminants, and eluted with imidazole-containing buffer. Binding affinity can be measured using surface plasmon resonance.
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| Cell Assay |
In vitro cell-based assays using Hexa-His typically involve expression of His-tagged recombinant proteins in cell lines (e.g., HEK293, CHO, E. coli). Cells are transfected with expression vectors encoding His-tagged proteins, followed by protein extraction and purification using IMAC. The purified protein is then characterized by SDS-PAGE and Western blot using anti-His antibodies.
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| Animal Protocol |
In vivo animal studies are not typically performed with Hexa-His as it is a peptide tag rather than a therapeutic agent. His-tagged proteins may be administered to animals for pharmacokinetic or efficacy studies, with the His tag serving as a detection epitope for tracking the protein in vivo.
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| ADME/Pharmacokinetics |
Hexa-His has a molecular weight of 840.85 g/mol and formula C₃₆H₄₄N₁₈O₇. It is soluble in water and should be stored at -20°C as a powder. The peptide is stable and does not exhibit systemic pharmacokinetics as it is not administered as a therapeutic agent.
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| Toxicity/Toxicokinetics |
Hexa-His is considered non-toxic as a peptide. Standard laboratory safety precautions should be followed when handling. No significant toxicity has been reported.
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| References | |
| Additional Infomation |
Hexa-His is widely used in molecular biology and protein biochemistry as the 6xHis tag for recombinant protein purification. It is commercially available as a synthetic peptide and is also known as His-Tag, Histidine hexapeptide, and H-His-His-His-His-His-His-OH. Not approved for therapeutic use; intended for research applications only.
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| Molecular Formula |
C36H44N18O7
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|---|---|
| Molecular Weight |
840.850960000001
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| Exact Mass |
840.364
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| CAS # |
64134-30-1
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| Related CAS # |
Hexa-His TFA;1456728-20-3
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| PubChem CID |
10123561
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| Appearance |
White to off-white solid powder
|
| LogP |
-5.7
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| Hydrogen Bond Donor Count |
13
|
| Hydrogen Bond Acceptor Count |
14
|
| Rotatable Bond Count |
23
|
| Heavy Atom Count |
61
|
| Complexity |
1500
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| Defined Atom Stereocenter Count |
6
|
| SMILES |
NC(C(NC(C(NC(C(NC(C(NC(C(NC(C(O)=O)CC1N=CNC=1)=O)CC1N=CNC=1)=O)CC1N=CNC=1)=O)CC1N=CNC=1)=O)CC1N=CNC=1)=O)CC1N=CNC=1
|
| InChi Key |
XSYUPRQVAHJETO-WPMUBMLPSA-N
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| InChi Code |
InChI=1S/C36H44N18O7/c37-25(1-19-7-38-13-44-19)31(55)50-26(2-20-8-39-14-45-20)32(56)51-27(3-21-9-40-15-46-21)33(57)52-28(4-22-10-41-16-47-22)34(58)53-29(5-23-11-42-17-48-23)35(59)54-30(36(60)61)6-24-12-43-18-49-24/h7-18,25-30H,1-6,37H2,(H,38,44)(H,39,45)(H,40,46)(H,41,47)(H,42,48)(H,43,49)(H,50,55)(H,51,56)(H,52,57)(H,53,58)(H,54,59)(H,60,61)/t25-,26-,27-,28-,29-,30-/m0/s1
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| Chemical Name |
(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-amino-3-(1H-imidazol-5-yl)propanoyl]amino]-3-(1H-imidazol-5-yl)propanoyl]amino]-3-(1H-imidazol-5-yl)propanoyl]amino]-3-(1H-imidazol-5-yl)propanoyl]amino]-3-(1H-imidazol-5-yl)propanoyl]amino]-3-(1H-imidazol-5-yl)propanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~50 mg/mL (~59.46 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (118.93 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.1893 mL | 5.9464 mL | 11.8927 mL | |
| 5 mM | 0.2379 mL | 1.1893 mL | 2.3785 mL | |
| 10 mM | 0.1189 mL | 0.5946 mL | 1.1893 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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