| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg | |||
| 250mg | |||
| Other Sizes |
| Targets |
Heptamethine cyanine dye-1 is a fluorescent labeling reagent that is used to label biomolecules for detection and imaging. It has excellent cell membrane permeability and tumor-targeting properties. The dye is commonly used in in vivo imaging, tumor detection, and drug delivery studies. It does not have a specific biological target like an enzyme.
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|---|---|
| ln Vitro |
In vitro, Heptamethine cyanine dye-1 is used for labeling cells and biomolecules. Its near-infrared fluorescence properties allow for deep tissue imaging with minimal autofluorescence. The dye is stable and photostable, making it suitable for long-term imaging applications. It is used in fluorescence microscopy, flow cytometry, and as a contrast agent in various assays.
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| ln Vivo |
In vivo, Heptamethine cyanine dye-1 is used for fluorescence imaging in small animal models. Its NIR fluorescence enables deep tissue penetration, making it ideal for visualizing tumors, tracking cells, and studying biodistribution. The dye's tumor-targeting properties allow for the specific accumulation in tumor tissues, which is valuable for cancer research.
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| Enzyme Assay |
In vitro assays using Heptamethine cyanine dye-1 primarily involve labeling and imaging. For example, cells are incubated with the dye, washed, and then imaged using a fluorescence microscope or analyzed by flow cytometry. The dye's fluorescence is measured at its excitation and emission wavelengths (typically in the NIR range).
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| Cell Assay |
For cellular assays, Heptamethine cyanine dye-1 is used to stain live cells. Cells are incubated with the dye at a specific concentration for a set period. After washing, the cells can be imaged live or fixed. The dye's fluorescence intensity is a measure of its uptake and can be used to assess cell viability or track cellular processes.
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| Animal Protocol |
In vivo imaging studies are performed using small animal imaging systems. Heptamethine cyanine dye-1 is injected intravenously into mice. At various time points post-injection, the animals are anesthetized and imaged using appropriate excitation and emission filters. The fluorescence signal is quantified and analyzed to determine the dye's distribution and accumulation in tissues.
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| ADME/Pharmacokinetics |
Heptamethine cyanine dye-1 has a molecular weight of 739.17 g/mol and a chemical formula of C42H44ClIN2. It is a solid compound that is soluble in DMSO. As a fluorescent dye, its pharmacokinetics are characterized by its distribution and clearance from the body. The dye is typically cleared through the renal system.
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| Toxicity/Toxicokinetics |
Heptamethine cyanine dye-1 is generally considered non-toxic for labeling applications at the concentrations used. The dye itself has low cytotoxicity. However, as with any chemical, standard safety precautions should be followed when handling it. It is for research use only and not for human therapeutic or diagnostic use.
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| Additional Infomation |
Heptamethine cyanine dye-1 is a near-infrared cyanine dye for fluorescence imaging in biological systems. It is a versatile tool for in vivo imaging, tumor detection, and drug delivery studies. Its excellent cell membrane permeability and NIR fluorescence make it a valuable probe for non-invasive imaging of biological processes.
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| Molecular Formula |
C42H44N2CL+.I-
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|---|---|
| Molecular Weight |
739.169
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| Exact Mass |
738.223
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| CAS # |
162411-29-2
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| PubChem CID |
10395228
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| Appearance |
Light brown to black solid powder
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
46
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| Complexity |
1200
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CCN\1C2=C(C3=CC=CC=C3C=C2)C(/C1=C\C=C\4/CCCC(=C4Cl)/C=C/C5=[N+](C6=C(C5(C)C)C7=CC=CC=C7C=C6)CC)(C)C.[I-]
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| InChi Key |
CNQCCCLLXKARPK-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C42H44ClN2.HI/c1-7-44-34-24-20-28-14-9-11-18-32(28)38(34)41(3,4)36(44)26-22-30-16-13-17-31(40(30)43)23-27-37-42(5,6)39-33-19-12-10-15-29(33)21-25-35(39)45(37)8-2;/h9-12,14-15,18-27H,7-8,13,16-17H2,1-6H3;1H/q+1;/p-1
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| Chemical Name |
(2E)-2-[(2E)-2-[2-chloro-3-[(E)-2-(3-ethyl-1,1-dimethylbenzo[e]indol-3-ium-2-yl)ethenyl]cyclohex-2-en-1-ylidene]ethylidene]-3-ethyl-1,1-dimethylbenzo[e]indole;iodide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~67.64 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (3.38 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.3529 mL | 6.7643 mL | 13.5287 mL | |
| 5 mM | 0.2706 mL | 1.3529 mL | 2.7057 mL | |
| 10 mM | 0.1353 mL | 0.6764 mL | 1.3529 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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