| Size | Price | Stock | Qty |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| Other Sizes |
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| ln Vitro |
In the damaged area of myenteric neurons isolated from guinea pig longitudinal muscle strips, hemicholinium 3 (HC-3) inhibits nAChRs[2]. With a Ki value of 13.3 μM, hemicholinium 3 (HC-3) inhibits [3H]choline solution in NCI-H69 cells [3]. At a high affinity choline dosage (IC50=18 nM), hemicholinium 3 (HC-3) (1 mM) strongly reduces sodium cell inhibition in NCI-H69 cells [4].
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|---|---|
| ln Vivo |
Learning about space is hampered by hemicholinium-3. The capacity to learn spatially is impaired by hemicholinium-3 (2.5, 5.0 μg/bag/ICV; 1 hour before training) computing devices [5].
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| Cell Assay |
Cell Viability Assay [3]
Cell Types: The Human Small Cell Lung Cancer Cell Line NCI-H69 Tested Concentrations: 1 mM Incubation Duration: Add daily for 2 days. Experimental Results: Dramatically inhibited cell viability. |
| References |
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| Additional Infomation |
A potent inhibitor of the high affinity uptake system for CHOLINE. It has less effect on the low affinity uptake system. Since choline is one of the components of ACETYLCHOLINE, treatment with hemicholinium can deplete acetylcholine from cholinergic terminals. Hemicholinium 3 is commonly used as a research tool in animal and in vitro experiments.
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| Molecular Formula |
C24H34BR2N2O4
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|---|---|
| Molecular Weight |
574.35
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| Exact Mass |
572.089
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| CAS # |
312-45-8
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| PubChem CID |
9399
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| Appearance |
White to off-white solid powder
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| Melting Point |
180 °C
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
32
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| Complexity |
544
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
OPYKHUMNFAMIBL-UHFFFAOYSA-L
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| InChi Code |
InChI=1S/C24H34N2O4.2BrH/c1-25(2)13-15-29-23(27,17-25)21-9-5-19(6-10-21)20-7-11-22(12-8-20)24(28)18-26(3,4)14-16-30-24;;/h5-12,27-28H,13-18H2,1-4H3;2*1H/q+2;;/p-2
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| Chemical Name |
2-[4-[4-(2-hydroxy-4,4-dimethylmorpholin-4-ium-2-yl)phenyl]phenyl]-4,4-dimethylmorpholin-4-ium-2-ol;dibromide
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| Synonyms |
HC-3; HC 3; HC3
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~33.33 mg/mL (~58.03 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 10 mg/mL (17.41 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication (<60°C).
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.7411 mL | 8.7055 mL | 17.4110 mL | |
| 5 mM | 0.3482 mL | 1.7411 mL | 3.4822 mL | |
| 10 mM | 0.1741 mL | 0.8705 mL | 1.7411 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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