| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
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| ln Vitro |
Similar to fura-2, HC-056456 reduced the rise in [Ca2+]i caused by flat ground depotentiation. Reversibly and selectively lowering CatSper current captured via the patch-clamp button is another feature of HC-056456. A CatSper-null pharmacological phenotype is produced by HC-056456. The hyperactivated agent rapidly loses its hairy evaporability upon acute administration of HC-056456, suggesting that continuous Ca2+ input via CatSper channels is required to keep the hyperactivated agent active. CatSper current is selectively and reversibly boiled by HC-056456. Utilizing patch-clamp recordings, investigate the reversibility and intermittency of CatSper-dependent current flow by HC-056456. Twenty μM HC-056456 inhibited about half of the observed current (the calculated IC50 is closer to 15 μM). Theoretically, the remaining HC-056456-resistant current could still be explained by CatSper channel heterogeneity. It was also investigated how HC-056456 affected KSper channels, which are the other main cation channel seen in the membrane clamp. A subsequent application of 50 μM HC-056456 caused this current to be partially blocked. The calculated IC50 for HC-056456's impact on KSper is near 40 μM [1].
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| References |
| Molecular Formula |
C12H6N2O4S2
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|---|---|
| Molecular Weight |
306.31
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| Exact Mass |
305.977
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| CAS # |
7733-96-2
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| PubChem CID |
573747
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| Appearance |
White to off-white solid powder
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| LogP |
2.688
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
20
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| Complexity |
409
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
RUQGCDMXFBOTMW-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C12H6N2O4S2/c15-11(7-3-1-5-19-7)9-10(14(17)18-13-9)12(16)8-4-2-6-20-8/h1-6H
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| Chemical Name |
[5-oxido-4-(thiophene-2-carbonyl)-1,2,5-oxadiazol-5-ium-3-yl]-thiophen-2-ylmethanone
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| Synonyms |
HC056456; HC 056456; HC-056456
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~125 mg/mL (~408.07 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (6.79 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (6.79 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.2647 mL | 16.3233 mL | 32.6467 mL | |
| 5 mM | 0.6529 mL | 3.2647 mL | 6.5293 mL | |
| 10 mM | 0.3265 mL | 1.6323 mL | 3.2647 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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