| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 250mg | |||
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| Other Sizes |
| Targets |
H2S Donor 5a exerts biological effects by releasing hydrogen sulfide (H2S), a gaseous signaling molecule; [1]
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|---|---|
| ln Vitro |
1. H2S Donor 5a is a cysteine-activated H2S donor that releases H2S in a concentration- and time-dependent manner upon reaction with L-cysteine (Cys): in phosphate-buffered saline (PBS, pH 7.4) containing 1 mM L-cysteine, H2S Donor 5a (100 μM) released H2S with an initial burst phase (0–30 min) followed by sustained release, reaching a cumulative H2S release of ~85 μM after 2 hours (detected by the methylene blue method) [1]
2. In the absence of L-cysteine, H2S Donor 5a showed minimal H2S release (<5 μM over 2 hours) in PBS (pH 7.4), demonstrating high selectivity for cysteine activation [1] 3. H2S Donor 5a exhibited good chemical stability in PBS (pH 7.4) without cysteine, with less than 10% degradation after 24 hours at 37°C [1] |
| References | |
| Additional Infomation |
1. Hydrogen sulfide (H2S) is an important endogenous gaseous signaling molecule that participates in regulating vascular tone, inflammation and cellular redox balance; however, conventional H2S donors (e.g., sodium hydrosulfide, NaHS) release H2S rapidly and nonselectively, limiting their therapeutic applications [1]. 2. H2S donor 5a is a novel aryl thiocarbamate-based H2S donor designed to achieve controlled H2S release when reacting with cysteine (a biothiol abundant in mammalian cells); the reaction mechanism involves the nucleophilic attack of the carbonyl carbon of H2S donor 5a by the cysteine thiol group, followed by CS bond cleavage, releasing H2S and phenolic byproducts [1]. 3. The cysteine-activated release properties of H2S donor 5a overcome the limitations of nonselective H2S donors, making it a promising tool compound for studying the biological functions of H2S and for developing H2S-based compounds. Potential lead compounds for therapeutic drugs [1]
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| Molecular Formula |
C14H11NO2S
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|---|---|
| Molecular Weight |
257.307642221451
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| Exact Mass |
257.051
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| CAS # |
134861-13-5
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| PubChem CID |
14952493
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| Appearance |
White to off-white solid powder
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| LogP |
3.295
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
18
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| Complexity |
294
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
IJYATHJDICJZLJ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C14H11NO2S/c16-13(11-7-3-1-4-8-11)15-18-14(17)12-9-5-2-6-10-12/h1-10H,(H,15,16)
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| Chemical Name |
S-benzamido benzenecarbothioate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~388.64 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (9.72 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.8864 mL | 19.4318 mL | 38.8636 mL | |
| 5 mM | 0.7773 mL | 3.8864 mL | 7.7727 mL | |
| 10 mM | 0.3886 mL | 1.9432 mL | 3.8864 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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