Size | Price | Stock | Qty |
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50mg |
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100mg |
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250mg |
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500mg |
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1g |
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Other Sizes |
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Purity: ≥98%
ln Vitro |
Advice (this is our suggested plan; it should be adjusted to meet your individual needs; it is merely a guide). 1. To create a 10 mM extraction solution, dissolve H2DCFDA in DMSO. Then, extract again before to usage. 2. After staining the cells for 30 minutes at 37°C in the dark with PBS solution containing 5 μM H2DCFDA and 0.05% trypsin-EDTA solution, the cells were suspended in new culture medium and subjected to an instant flow cytometry analysis. 3. If required, employ the H2DCFDA probe in conjunction with the ROS-insensitive fluorescein dye DCFDA modifier as an improved control. The staining process is identical to that of H2DCFDA [1].
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References |
[1]. Lyublinskaya OG, et al. Redox environment in stem and differentiated cells: A quantitative approach. Redox Biol. 2017 Aug;12:758-769
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Molecular Formula |
C24H16CL2O7
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Molecular Weight |
487.2856
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CAS # |
4091-99-0
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Related CAS # |
4091-99-0;
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SMILES |
ClC1=C(C([H])=C2C(=C1[H])C([H])(C1=C([H])C([H])=C([H])C([H])=C1C(=O)O[H])C1=C([H])C(=C(C([H])=C1O2)OC(C([H])([H])[H])=O)Cl)OC(C([H])([H])[H])=O
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~125 mg/mL (~256.52 mM)
Ethanol : ~20 mg/mL (~41.04 mM) |
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Solubility (In Vivo) |
Solubility in Formulation 1: 2.08 mg/mL (4.27 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (4.27 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (4.27 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.0522 mL | 10.2608 mL | 20.5217 mL | |
5 mM | 0.4104 mL | 2.0522 mL | 4.1043 mL | |
10 mM | 0.2052 mL | 1.0261 mL | 2.0522 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.