| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
H-Pro-Gly-OH does not have a specific biological target as a drug. It is an endogenous metabolite. The compound may be recognized by certain peptidases and amino acid transporters, but it is not designed to interact with specific receptors or enzymes for therapeutic purposes. Its primary value is as a research tool for studying peptide metabolism, proline biology, and the role of dipeptides in cellular function.
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|---|---|
| ln Vitro |
In vitro, H-Pro-Gly-OH is used as a substrate in enzymatic assays to study the activity of peptidases. It may also be used in studies of amino acid transport and metabolism. The compound does not exhibit significant pharmacological activities such as receptor binding or enzyme inhibition. Its role in research is as a reagent for studying peptide metabolism, proline biology, and the role of dipeptides in biological systems.
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| ln Vivo |
H-Pro-Gly-OH is not a pharmacologically active compound and therefore does not have defined in vivo activity as a therapeutic agent. It is an endogenously produced metabolite. When administered to animals, it would likely be metabolized by peptidases to release proline and glycine. Its primary value is as a research tool for studying peptide metabolism, proline biology, and the role of dipeptides in cellular function.
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| Enzyme Assay |
In vitro enzyme assays for H-Pro-Gly-OH are typically designed to study peptidase or protease activity. A standard protocol involves incubating the compound with an enzyme preparation in a suitable buffer. The hydrolysis of the dipeptide bond releases proline and glycine, which can be quantified by HPLC or mass spectrometry. The progress of the reaction can be monitored, and kinetic parameters can be determined. These assays are used to characterize the substrate specificity of peptidases.
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| Cell Assay |
In vitro cellular assays using H-Pro-Gly-OH are limited because the compound is primarily a metabolite. However, it can be used in cell culture studies to investigate peptide transport and metabolism. Cells are cultured in media supplemented with the compound, and its uptake and hydrolysis are monitored. The effects of increased proline and glycine availability on cellular metabolism can be assessed.
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| Animal Protocol |
In vivo animal studies with H-Pro-Gly-OH are not typically conducted for therapeutic purposes. However, it may be used in metabolic studies to investigate proline and glycine metabolism. A typical protocol involves administration of the compound to rodents, followed by blood and tissue sampling to measure its levels and metabolites. These studies help to understand the metabolism of proline-containing peptides.
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| ADME/Pharmacokinetics |
As a small, hydrophilic dipeptide, H-Pro-Gly-OH is expected to be rapidly metabolized in the body. Its pharmacokinetic properties are characteristic of small peptides, with rapid clearance and short half-life. Detailed pharmacokinetic data are not typically reported, as the compound is primarily a metabolite rather than a drug candidate.
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| Toxicity/Toxicokinetics |
H-Pro-Gly-OH is generally considered to have low toxicity, consistent with its status as an endogenous metabolite. It is a dipeptide composed of the amino acids proline and glycine. Standard laboratory safety precautions, including the use of personal protective equipment, are recommended. No significant systemic toxicity is anticipated at typical research doses.
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| Additional Infomation |
L-Prolylglycine is a dipeptide composed of an L-prolyl group attached to the α-amino nitrogen atom of glycine. It is the zwitterion tautomer of L-prolylglycine. Prolylglycine has been reported to exist in Homo sapiens, and relevant data are available for reference.
H-Pro-Gly-OH (L-Prolylglycine, CAS 2578-57-6) is a dipeptide composed of L-proline and glycine. Its molecular formula is C₇H₁₂N₂O₃ and its molecular weight is 172.18 g/mol. It is an endogenously produced metabolite. It is used in biochemical research to study peptide metabolism and the role of proline-containing peptides in biological systems. It is intended for research use only. |
| Molecular Formula |
C7H12N2O3
|
|---|---|
| Molecular Weight |
172.1818
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| Exact Mass |
172.085
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| CAS # |
2578-57-6
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| PubChem CID |
6426709
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| Appearance |
White to off-white solid powder
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| Density |
1.266 g/cm3
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| Boiling Point |
468.5ºC at 760 mmHg
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| Melting Point |
236℃
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| Flash Point |
237.1ºC
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| Vapour Pressure |
4.5E-10mmHg at 25°C
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| Index of Refraction |
1.511
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| LogP |
-3
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
12
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| Complexity |
193
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| Defined Atom Stereocenter Count |
1
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| SMILES |
C1C[C@H](NC1)C(=O)NCC(=O)O
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| InChi Key |
RNKSNIBMTUYWSH-YFKPBYRVSA-N
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| InChi Code |
InChI=1S/C7H12N2O3/c10-6(11)4-9-7(12)5-2-1-3-8-5/h5,8H,1-4H2,(H,9,12)(H,10,11)/t5-/m0/s1
|
| Chemical Name |
2-[[(2S)-pyrrolidine-2-carbonyl]amino]acetic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~50 mg/mL (~290.39 mM)
DMSO :< 1 mg/mL |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (580.79 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.8079 mL | 29.0394 mL | 58.0788 mL | |
| 5 mM | 1.1616 mL | 5.8079 mL | 11.6158 mL | |
| 10 mM | 0.5808 mL | 2.9039 mL | 5.8079 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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