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| Other Sizes |
| Targets |
H-Lys(Z)-OtBu.HCl is classified as a protected amino acid ester and lysine analogue. It does not have a specific biological receptor target. Its primary application is as a building block in peptide synthesis for introducing lysine residues with orthogonal N-terminal and C-terminal protection. The Z group provides protection for the ε-amino group that is stable to acid but removable by hydrogenolysis, while the tert-butyl ester provides acid-labile C-terminal protection.
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| ln Vitro |
Commercial ergot supplements have been made from amino acids and their derivatives. They affect the release of anabolic hormones, the availability of fuel for activity, the ability to think clearly under pressure, and the prevention of muscular damage brought on by exertion. They are regarded as advantageous synergistic food ingredients [1].
In vitro activity for H-Lys(Z)-OtBu.HCl is primarily as a peptide synthesis reagent. Amino acids and amino acid derivatives have been commercially used as ergogenic supplements. They influence the secretion of anabolic hormones, supply of fuel during exercise, mental performance during stress-related tasks and prevent exercise-induced muscle damage. H-Lys(Z)-OtBu.HCl itself is not directly biologically active; its utility is in preparing lysine-containing peptides for subsequent biological evaluation. |
| ln Vivo |
In vivo activity data for H-Lys(Z)-OtBu.HCl as a standalone compound is not applicable. It is a protected amino acid ester used exclusively as a research building block and is not intended for direct in vivo administration. Peptides synthesized using this building block may be evaluated in animal models for various biological activities after the protecting groups are removed.
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| Enzyme Assay |
In vitro SPPS protocols for H-Lys(Z)-OtBu.HCl involve standard peptide synthesis chemistry. The compound is activated with coupling reagents such as HATU or HBTU and DIPEA in DMF, then coupled to a growing peptide chain. The tert-butyl ester is removed with TFA, and the Z group is removed by hydrogenolysis over Pd/C. Purity is typically high.
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| Cell Assay |
Cell-based protocols are not directly applicable to H-Lys(Z)-OtBu.HCl as it is a protected amino acid derivative and not typically used in cell culture. Lysine-containing peptides synthesized using this reagent can be tested in cell culture after deprotection and purification. Peptides are dissolved in DMSO or appropriate buffers and added to cells at concentrations of 0.1-100 µM for 24-72 hours.
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| Animal Protocol |
Animal studies with H-Lys(Z)-OtBu.HCl are not conducted directly. Peptides containing lysine residues synthesized using this building block may be evaluated in vivo. Protocols involve administration of the peptide via appropriate routes in animal models. Pharmacokinetic sampling, efficacy assessment, and toxicological evaluation follow standard preclinical study designs.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for H-Lys(Z)-OtBu.HCl as a standalone compound is not applicable. The compound has a molecular weight of 372.89 g/mol, formula C18H28N2O4·HCl, and CAS number 5978-22-3. Storage is recommended in a cool, dry place. The Z group is removed by hydrogenolysis and the tert-butyl ester by acid.
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| Toxicity/Toxicokinetics |
Limited toxicological data is available for H-Lys(Z)-OtBu.HCl. The compound is for research use only and is not intended for human therapeutic use. Standard safety precautions for handling protected amino acids should be observed. No specific toxicity studies have been reported.
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| References | |
| Additional Infomation |
H-Lys(Z)-OtBu.HCl (CAS#: 5978-22-3) is also known as Nε-Benzyloxycarbonyl-L-lysine tert-butyl ester hydrochloride. Its molecular formula is C18H28N2O4·HCl. It is a protected lysine building block used in peptide synthesis. It has no approved therapeutic indications.
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| Molecular Formula |
C18H29CLN2O4
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|---|---|
| Molecular Weight |
372.8869
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| Exact Mass |
372.181
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| CAS # |
5978-22-3
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| PubChem CID |
13536460
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| Appearance |
White to off-white powder
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| Boiling Point |
469.6ºC at 760 mmHg
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| Flash Point |
237.8ºC
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| LogP |
4.645
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
11
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| Heavy Atom Count |
25
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| Complexity |
387
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| Defined Atom Stereocenter Count |
1
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| SMILES |
Cl[H].O(C([C@]([H])(C([H])([H])C([H])([H])C([H])([H])C([H])([H])N([H])C(=O)OC([H])([H])C1C([H])=C([H])C([H])=C([H])C=1[H])N([H])[H])=O)C(C([H])([H])[H])(C([H])([H])[H])C([H])([H])[H]
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| InChi Key |
HEMZMPXAQORYDR-RSAXXLAASA-N
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| InChi Code |
InChI=1S/C18H28N2O4.ClH/c1-18(2,3)24-16(21)15(19)11-7-8-12-20-17(22)23-13-14-9-5-4-6-10-14;/h4-6,9-10,15H,7-8,11-13,19H2,1-3H3,(H,20,22);1H/t15-;/m0./s1
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| Chemical Name |
tert-butyl (2S)-2-amino-6-(phenylmethoxycarbonylamino)hexanoate;hydrochloride
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| Synonyms |
H-Lys(Z)-OtBu.HCl
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~100 mg/mL (~268.18 mM)
DMSO : ~100 mg/mL (~268.18 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.70 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.70 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (6.70 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6818 mL | 13.4088 mL | 26.8176 mL | |
| 5 mM | 0.5364 mL | 2.6818 mL | 5.3635 mL | |
| 10 mM | 0.2682 mL | 1.3409 mL | 2.6818 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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