| Size | Price | Stock | Qty |
|---|---|---|---|
| 10g |
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| Other Sizes |
| Targets |
H-His-Ome.2HCl is classified as a histidine analogue and protected amino acid ester. It does not have a specific biological receptor target. Its primary application is as a building block in peptide synthesis for introducing histidine residues with C-terminal methyl ester protection. The methyl ester provides C-terminal protection that can be selectively hydrolyzed under basic conditions.
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| ln Vitro |
Commercial ergot supplements have been made from amino acids and their derivatives. They affect the release of anabolic hormones, the availability of fuel for activity, the ability to think clearly under pressure, and the prevention of muscular damage brought on by exertion. They are regarded as advantageous synergistic food ingredients [1].
In vitro activity for H-His-Ome.2HCl is primarily as a peptide synthesis reagent. Amino acids and amino acid derivatives have been commercially used as ergogenic supplements. They influence the secretion of anabolic hormones, supply of fuel during exercise, mental performance during stress-related tasks and prevent exercise-induced muscle damage. H-His-Ome.2HCl itself is not directly biologically active; its utility is in preparing histidine-containing peptides for subsequent biological evaluation. |
| ln Vivo |
In vivo activity data for H-His-Ome.2HCl as a standalone compound is not applicable. It is a protected amino acid ester used exclusively as a research building block and is not intended for direct in vivo administration. It is used in the preparation of optically pure L-(+)-Ergothioneine. Peptides synthesized using this building block may be evaluated in animal models after deprotection.
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| Enzyme Assay |
In vitro SPPS protocols for H-His-Ome.2HCl involve standard peptide synthesis chemistry. The compound is activated with coupling reagents such as HATU or HBTU and DIPEA in DMF, then coupled to a growing peptide chain. The methyl ester can be hydrolyzed under basic conditions. Purity is typically high.
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| Cell Assay |
Cell-based protocols are not directly applicable to H-His-Ome.2HCl as it is a protected amino acid derivative and not typically used in cell culture. Histidine-containing peptides synthesized using this reagent can be tested in cell culture after deprotection and purification. Peptides are dissolved in DMSO or appropriate buffers and added to cells at concentrations of 0.1-100 µM for 24-72 hours.
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| Animal Protocol |
Animal studies with H-His-Ome.2HCl are not conducted directly. Peptides containing histidine residues synthesized using this building block may be evaluated in vivo. Protocols involve administration of the peptide via appropriate routes in animal models. Pharmacokinetic sampling, efficacy assessment, and toxicological evaluation follow standard preclinical study designs.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for H-His-Ome.2HCl as a standalone compound is not applicable. The compound has a molecular weight of 242.10 g/mol, formula C7H11N3O2·2HCl, and CAS number 7389-87-9. Melting point is 207°C (dec.). Storage is recommended at room temperature in a cool, dark place below 15°C.
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| Toxicity/Toxicokinetics |
Limited toxicological data is available for H-His-Ome.2HCl. The compound is for research use only and is not intended for human therapeutic use. Standard safety precautions for handling protected amino acids should be observed. No specific toxicity studies have been reported.
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| References |
[1]. Luckose F, et al. Effects of amino acid derivatives on physical, mental, and physiological activities. Crit Rev Food Sci Nutr. 2015;55(13):1793-1144.
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| Additional Infomation |
H-His-Ome.2HCl (CAS#: 7389-87-9) is also known as L-Histidine methyl ester dihydrochloride, Methyl L-histidinate dihydrochloride, and (2S)-2-amino-3-(1H-imidazol-4-yl)propanoate dihydrochloride. Its molecular formula is C7H11N3O2·2HCl. It is a histidine analogue used in peptide synthesis. It has no approved therapeutic indications.
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| Molecular Formula |
C7H13CL2N3O2
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|---|---|
| Molecular Weight |
242.1030
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| Exact Mass |
241.038
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| CAS # |
7389-87-9
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| PubChem CID |
2723645
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| Appearance |
White to off-white powder
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| Boiling Point |
368.2ºC at 760 mmHg
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| Melting Point |
207 °C (dec.)(lit.)
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| Flash Point |
176.5ºC
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| Index of Refraction |
10 ° (C=2, H2O)
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| LogP |
1.756
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
14
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| Complexity |
163
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| Defined Atom Stereocenter Count |
1
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| SMILES |
Cl[H].Cl[H].O(C([H])([H])[H])C([C@]([H])(C([H])([H])C1=C([H])N=C([H])N1[H])N([H])[H])=O
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| InChi Key |
DWAYENIPKPKKMV-ILKKLZGPSA-N
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| InChi Code |
InChI=1S/C7H11N3O2.2ClH/c1-12-7(11)6(8)2-5-3-9-4-10-5;;/h3-4,6H,2,8H2,1H3,(H,9,10);2*1H/t6-;;/m0../s1
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| Chemical Name |
methyl (2S)-2-amino-3-(1H-imidazol-5-yl)propanoate;dihydrochloride
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| Synonyms |
Methyl L-histidinate dihydrochloride; H-His-Ome.2HCl
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~100 mg/mL (~413.05 mM)
DMSO : ~7.69 mg/mL (~31.76 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 0.77 mg/mL (3.18 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 7.7 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 0.77 mg/mL (3.18 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 7.7 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 0.77 mg/mL (3.18 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.1305 mL | 20.6526 mL | 41.3052 mL | |
| 5 mM | 0.8261 mL | 4.1305 mL | 8.2610 mL | |
| 10 mM | 0.4131 mL | 2.0653 mL | 4.1305 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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