| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| Targets |
H-Gly-Pro-AMC targets Dipeptidyl peptidase IV (DPP-IV), also known as CD26. DPP-IV is a serine protease that cleaves N-terminal dipeptides from polypeptides. The compound serves as a substrate for DPP-IV, and its cleavage releases the fluorescent AMC group. It does not have a therapeutic target but is a research tool for measuring DPP-IV activity in vitro and in biological samples.
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| ln Vitro |
Gly-Pro-AMC hydrobromide (50 µM; 20 minutes; 37 °C) can be employed as a particular fluorogenic substrate for DPP-IV activity detection [1]. The hydrobromide Gly-Pro-AMC exhibits fluorescence. The excitation wavelength of 350 nm and the fluorescence emission spectrum detection of 450 nm are the conditions for fluorescence detection[1].
In vitro, H-Gly-Pro-AMC is used as a fluorogenic substrate to measure DPP-IV activity. The compound is incubated with a sample containing DPP-IV, and the release of AMC is monitored by fluorescence (excitation at 350-380 nm, emission at 450-460 nm). The rate of fluorescence increase is proportional to enzyme activity. It is highly sensitive and is used for inhibitor screening and for measuring DPP-IV activity in biological fluids. |
| ln Vivo |
H-Gly-Pro-AMC is not a pharmacologically active compound and therefore does not have defined in vivo activity as a therapeutic agent. It is a research tool used to measure DPP-IV activity in biological samples. When administered to animals, it would likely be cleaved by DPP-IV to release the AMC fluorophore. Its primary value is as a diagnostic and research reagent for studying DPP-IV activity in health and disease.
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| Enzyme Assay |
In vitro enzyme assays for H-Gly-Pro-AMC are designed to measure DPP-IV activity. A standard protocol involves incubating the substrate with a sample containing DPP-IV (e.g., plasma, tissue homogenate, or purified enzyme) in a suitable buffer at 37°C. The release of AMC is monitored continuously or at fixed time points using a fluorometer with excitation at 350-380 nm and emission at 450-460 nm. The initial velocity is calculated, and the enzyme activity is determined. These assays are used for inhibitor screening and for measuring DPP-IV activity in biological samples.
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| Cell Assay |
In vitro cellular assays using H-Gly-Pro-AMC are conducted to measure DPP-IV activity in cell cultures. Cells are lysed, or the culture supernatant is collected, and incubated with the substrate. The release of AMC is measured fluorometrically. These assays are used to study the regulation of DPP-IV expression and activity in various cell types and to screen for compounds that modulate DPP-IV activity in a cellular context.
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| Animal Protocol |
In vivo animal studies with H-Gly-Pro-AMC are not typically conducted for therapeutic purposes. However, it may be used in pharmacokinetic or pharmacodynamic studies to measure DPP-IV activity in vivo. A typical protocol involves administering the substrate to rodents, followed by blood sampling to measure the rate of AMC release. These studies help to assess the in vivo activity of DPP-IV and the efficacy of DPP-IV inhibitors.
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| ADME/Pharmacokinetics |
As a small, hydrophilic peptide substrate, H-Gly-Pro-AMC is expected to be rapidly cleared from the circulation. Its pharmacokinetic properties are characteristic of small peptides, with rapid clearance and short half-life. The compound is used primarily as a research reagent for in vitro assays, and detailed pharmacokinetic data are not typically reported.
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| Toxicity/Toxicokinetics |
H-Gly-Pro-AMC is generally considered to have low toxicity, consistent with its use as a research reagent. It is a peptide substrate with a coumarin fluorophore. Standard laboratory safety precautions, including the use of personal protective equipment, are recommended. No significant systemic toxicity is anticipated at typical research doses.
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| References | |
| Additional Infomation |
H-Gly-Pro-AMC (Gly-Pro-AMC hydrobromide, CAS 115035-46-6) is a fluorogenic peptide substrate comprising the dipeptide Gly-Pro linked to 7-amino-4-methylcoumarin (AMC). Its molecular formula is C₁₇H₂₀BrN₃O₄ and its molecular weight is approximately 410.26 g/mol. It is a specific fluorescent substrate for detecting Dipeptidyl peptidase IV (DPP-IV) activity. It is used in enzymatic assays for inhibitor screening and for measuring DPP-IV activity in biological fluids. It is intended for research use only.
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| Molecular Formula |
C17H19N3O4.HBR
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|---|---|
| Molecular Weight |
410.2624
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| Exact Mass |
409.064
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| CAS # |
115035-46-6
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| PubChem CID |
24802182
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| Appearance |
Off-white to light yellow solid powder
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| Boiling Point |
653.5ºC at 760 mmHg
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| Flash Point |
349ºC
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| Vapour Pressure |
1.56E-17mmHg at 25°C
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| LogP |
2.658
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
25
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| Complexity |
574
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| Defined Atom Stereocenter Count |
1
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| SMILES |
CC1=CC(=O)OC2=C1C=CC(=C2)NC(=O)[C@@H]3CCCN3C(=O)CN.Br
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| InChi Key |
PASXTQMFJRHMLJ-ZOWNYOTGSA-N
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| InChi Code |
InChI=1S/C17H19N3O4.BrH/c1-10-7-16(22)24-14-8-11(4-5-12(10)14)19-17(23)13-3-2-6-20(13)15(21)9-18;/h4-5,7-8,13H,2-3,6,9,18H2,1H3,(H,19,23);1H/t13-;/m0./s1
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| Chemical Name |
(2S)-1-(2-aminoacetyl)-N-(4-methyl-2-oxochromen-7-yl)pyrrolidine-2-carboxamide;hydrobromide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~243.75 mM)
H2O : ~100 mg/mL (~243.75 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.09 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.09 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (6.09 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4375 mL | 12.1874 mL | 24.3748 mL | |
| 5 mM | 0.4875 mL | 2.4375 mL | 4.8750 mL | |
| 10 mM | 0.2437 mL | 1.2187 mL | 2.4375 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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