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| Targets |
H-D-Orn-OH.HCl is an endogenous metabolite that targets amino acid metabolism pathways. It promotes the production of L-arginine and regulates intramuscular hormone levels. As a D-amino acid, it interacts with bacterial cell wall synthesis enzymes. It is studied for its neuroprotective properties and potential role in treating neurodegenerative diseases by supporting neuronal health. It also plays a role in urea cycle studies and nitrogen metabolism research.
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| ln Vitro |
In vitro studies have shown that D-ornithine hydrochloride can influence cellular metabolism. It has been found to regulate intramuscular hormone levels. D-ornithine is used in research on dry skin and insomnia. The compound serves as a valuable tool in studying branched-chain amino acid metabolism due to its structural similarity to ornithine. It is also used in bacterial cell wall studies as a constituent. Cell culture applications include studying amino acid transport and metabolism.
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| ln Vivo |
In vivo activity of D-ornithine hydrochloride has been studied in the context of sports nutrition and athletic performance. It is used as a dietary supplement to enhance athletic performance and recovery by supporting muscle metabolism and reducing fatigue. It has been studied for neuroprotective properties potentially aiding in neurodegenerative disease treatment. It may have effects on urea cycle function and nitrogen detoxification processes in animal models.
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| Enzyme Assay |
In vitro enzyme/receptor binding assays for H-D-Orn-OH.HCl typically involve studying its interactions with enzymes involved in amino acid metabolism, such as ornithine decarboxylase and arginase. Protocols involve incubating the compound with purified enzyme preparations in appropriate buffers at physiological pH, followed by measuring enzyme activity using spectrophotometric or chromatographic methods. For receptor binding studies, radiolabeled ornithine derivatives can be used to assess binding to amino acid transporters.
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| Cell Assay |
Cell culture protocols for H-D-Orn-OH.HCl involve dissolving the compound in sterile water or cell culture medium at concentrations ranging from 0.1-100 mM. Cells are treated with the compound for various time points (24-72 hours) and effects on cell viability, proliferation, or metabolic activity are assessed. For transport studies, cells are incubated with labeled ornithine and uptake is measured. The compound shows high solubility in water (~100 mg/mL), facilitating preparation of stock solutions.
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| Animal Protocol |
In vivo animal study protocols for D-ornithine hydrochloride involve administration via oral gavage or injection in saline or PBS. Dosing typically ranges from 50-500 mg/kg depending on the study. Blood and tissue samples are collected for analysis of metabolite levels and compound distribution. Studies may assess effects on athletic performance, muscle metabolism, or neuroprotection in appropriate animal models. Formulations should be freshly prepared for optimal results.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of H-D-Orn-OH.HCl are characteristic of small amino acids. The compound has a molecular weight of 168.62 g/mol and formula C₅H₁₃ClN₂O₂. It shows high aqueous solubility (~100 mg/mL in water and DMSO). It is readily absorbed and distributed to tissues. As an endogenous metabolite, it participates in normal amino acid metabolism pathways. Elimination is via renal excretion. The compound can be formulated in PBS for in vivo administration.
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| Toxicity/Toxicokinetics |
Toxicological data for H-D-Orn-OH.HCl indicates it is generally safe at research doses. As an endogenous metabolite, it has low inherent toxicity. Standard safety precautions for handling should be observed. The compound should be stored in a sealed, protected environment away from moisture. It is not approved for human therapeutic use and is for research purposes only. At high concentrations, cellular effects may be observed depending on cell type.
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| Additional Infomation |
D-ornithine hydrochloride is an organic molecular entity.
H-D-Orn-OH.HCl (CAS#: 16682-12-5) is also known as D-Ornithine hydrochloride, (R)-Ornithine hydrochloride, and (2R)-2,5-diaminopentanoic acid hydrochloride. Its molecular weight is 168.62 and formula is C₅H₁₃ClN₂O₂. The compound has a melting point of 239°C (dec.) and purity of ≥98%. It is an endogenous metabolite with applications in urea cycle research, sports nutrition, and neuroprotection studies. It is not approved for therapeutic use. |
| Molecular Formula |
C5H13CLN2O2
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|---|---|
| Molecular Weight |
168.6219
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| Exact Mass |
168.066
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| CAS # |
16682-12-5
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| PubChem CID |
443122
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| Appearance |
White to off-white powder
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| Density |
1.165g/cm3
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| Boiling Point |
308.7ºC at 760mmHg
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| Melting Point |
239ºC (dec.)(lit.)
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| Flash Point |
140.5ºC
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| Vapour Pressure |
0.00015mmHg at 25°C
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| LogP |
1.339
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
10
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| Complexity |
95
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| Defined Atom Stereocenter Count |
1
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| SMILES |
Cl[H].O([H])C([C@@]([H])(C([H])([H])C([H])([H])C([H])([H])N([H])[H])N([H])[H])=O
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| InChi Key |
GGTYBZJRPHEQDG-PGMHMLKASA-N
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| InChi Code |
InChI=1S/C5H12N2O2.ClH/c6-3-1-2-4(7)5(8)9;/h4H,1-3,6-7H2,(H,8,9);1H/t4-;/m1./s1
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| Chemical Name |
(2R)-2,5-diaminopentanoic acid;hydrochloride
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| Synonyms |
D-Ornithine hydrochloride; (R)-Ornithine hydrochloride; H-D-Orn-OH.HCl
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 100 mg/mL (~593.05 mM)
H2O : ~100 mg/mL (~593.05 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (593.05 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.9305 mL | 29.6525 mL | 59.3049 mL | |
| 5 mM | 1.1861 mL | 5.9305 mL | 11.8610 mL | |
| 10 mM | 0.5930 mL | 2.9652 mL | 5.9305 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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