| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg | |||
| Other Sizes |
| Targets |
H-Arg-4MβNA targets cathepsin H as a substrate. Cathepsin H is a lysosomal cysteine protease involved in protein degradation and processing. The compound is specifically designed for the detection of cathepsin H activity. Upon cleavage by cathepsin H, the compound releases a detectable fluorescent or chromogenic signal. H-Arg-4MβNA is used for enzyme activity detection in gel electrophoresis.
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| ln Vitro |
In vitro, H-Arg-4MβNA is a substrate for cathepsin H and is used for the detection of enzyme activity in gel electrophoresis. The compound is cleaved by cathepsin H to release a detectable fluorescent or chromogenic signal. It is employed for the detection of cathepsin H activity in various biological samples. H-Arg-4MβNA is a peptide substrate.
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| ln Vivo |
In vivo, H-Arg-4MβNA is used as a research tool for studying cathepsin H activity. The compound's utility in enzyme activity detection has been established. It is used in biochemical research for cathepsin H studies. Further in vivo studies are limited as the compound is primarily a laboratory reagent for enzyme assays.
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| Enzyme Assay |
In vitro enzyme assays for H-Arg-4MβNA involve measuring cathepsin H activity. The compound is incubated with cathepsin H-containing samples, and the release of 4-methoxy-β-naphthylamine is measured fluorometrically or chromogenically. For gel electrophoresis, the compound is incorporated into the gel or used as a substrate after electrophoresis. Enzyme activity is detected by fluorescence or color development. Assays are performed in appropriate buffer systems with positive controls such as known cathepsin H enzymes.
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| Cell Assay |
In vitro cell-based assays for H-Arg-4MβNA are conducted in cell lysates containing cathepsin H. Cells are cultured and lysed, and the lysates are incubated with the compound. Cathepsin H activity is measured by fluorescence or color development. Cell viability is assessed by standard assays. Experiments are performed with appropriate positive and negative controls.
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| Animal Protocol |
H-Arg-4MβNA in vivo studies are limited as the compound is primarily a laboratory reagent. Its utility in cathepsin H activity detection has been established. The compound is used in biochemical research for studying cathepsin H function. Studies are conducted in accordance with institutional guidelines for reagent use.
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| ADME/Pharmacokinetics |
H-Arg-4MβNA (MW 329.40 g/mol, C17H23N5O2) is a synthetic chromogenic substrate for cathepsin H. It consists of an arginine residue linked to 4-methoxy-β-naphthylamide. The compound is used for the detection of cathepsin H activity in gel electrophoresis. Pharmacokinetic properties are not typically characterized as it is a research reagent.
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| Toxicity/Toxicokinetics |
H-Arg-4MβNA is generally considered safe as a laboratory reagent. The compound is a substrate for cathepsin H used in enzyme activity assays. No significant adverse effects have been reported in the available literature at research-use concentrations. The compound is intended for research use only. Standard laboratory safety practices should be employed when handling this compound. Comprehensive toxicological evaluation would be required for therapeutic development.
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| Additional Infomation |
H-Arg-4MβNA is a synthetic chromogenic substrate for cathepsin H consisting of arginine linked to 4-methoxy-β-naphthylamide. It is cleaved by cathepsin H to release a detectable fluorescent or chromogenic signal. The compound is used for the detection of cathepsin H activity in gel electrophoresis. Its molecular formula is C17H23N5O2 with a molecular weight of 329.40 g/mol. All applications are limited to non-human research use.
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| Molecular Formula |
C17H23N5O2
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|---|---|
| Molecular Weight |
329.396823167801
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| Exact Mass |
329.185
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| CAS # |
60285-94-1
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| PubChem CID |
16218937
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| Appearance |
White to off-white solid powder
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| Density |
1.3±0.1 g/cm3
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| Index of Refraction |
1.631
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| LogP |
0.63
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
24
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| Complexity |
439
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| Defined Atom Stereocenter Count |
1
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| SMILES |
O=C([C@H](CCC/N=C(\N)/N)N)NC1C=C(C2C=CC=CC=2C=1)OC
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| InChi Key |
XVHWVDFGKDNOBO-AWEZNQCLSA-N
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| InChi Code |
InChI=1S/C17H23N5O2/c1-24-15-10-12(9-11-5-2-3-6-13(11)15)22-16(23)14(18)7-4-8-21-17(19)20/h2-3,5-6,9-10,14H,4,7-8,18H2,1H3,(H,22,23)(H4,19,20,21)/t14-/m0/s1
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| Chemical Name |
(2S)-2-amino-5-(diaminomethylideneamino)-N-(4-methoxynaphthalen-2-yl)pentanamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ≥ 125 mg/mL (~379.48 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 50 mg/mL (151.79 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0358 mL | 15.1791 mL | 30.3582 mL | |
| 5 mM | 0.6072 mL | 3.0358 mL | 6.0716 mL | |
| 10 mM | 0.3036 mL | 1.5179 mL | 3.0358 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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