| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
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| 100mg |
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| 250mg |
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| Other Sizes |
| Targets |
Guanosine 5'-diphosphate (GDP) targets ATP-sensitive K⁺ channels, which it activates. It also targets the hepcidin-ferroportin iron transport system by blocking their interaction, thereby functioning as a potential iron mobilizer. Additionally, it modulates the IL-6/STAT-3 signaling pathway. GDP is a substrate for various enzymes involved in nucleotide and nucleic acid metabolism.
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|---|---|
| ln Vitro |
In vitro, Guanosine 5'-diphosphate disodium salt is used to study the kinetics and characteristics of GTPases, such as those associated with G-protein coupled receptors (GPCRs). It activates ATP-sensitive K⁺ channels. It serves as a substrate for nucleoside diphosphate kinase and other enzymes involved in nucleotide metabolism. It is also used in studies of GTPase activity, where GDP-bound forms represent the inactive state of small GTPases.
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| ln Vivo |
In vivo, Guanosine 5'-diphosphate disodium salt (30 mL/kg, i.p., three times a week for two weeks) can improve turpentine-induced inflammatory anemia in mice. By blocking hepcidin-ferroportin interaction, it promotes iron mobilization and may alleviate anemia of inflammation. It modulates the IL-6/STAT-3 pathway, which is involved in the regulation of hepcidin expression during inflammation.
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| Enzyme Assay |
In vitro assays for Guanosine 5'-diphosphate disodium salt are conducted to study GTPase activity. GTPase assays typically use radiolabeled GTP or fluorescent GTP analogs to monitor GTP hydrolysis. GDP is used as a control or as a substrate for nucleotide exchange assays. Hepcidin-ferroportin binding assays can be performed using surface plasmon resonance (SPR) or ELISA to study the inhibitory effect of GDP on this interaction.
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| Cell Assay |
Cellular assays for Guanosine 5'-diphosphate disodium salt are performed using cell lines relevant to iron metabolism or GPCR signaling. Cells are treated with GDP at concentrations ranging from 0.1 to 10 mM. Iron mobilization is assessed by measuring intracellular iron levels using colorimetric or fluorescent iron indicators. STAT3 phosphorylation is measured by Western blotting or ELISA.
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| Animal Protocol |
In vivo animal studies for Guanosine 5'-diphosphate disodium salt have been conducted in male BALB/c mice using a turpentine-induced inflammatory anemia model. Mice are administered the compound intraperitoneally at 30 mL/kg three times a week for two weeks. Endpoints include hemoglobin levels, serum iron, hepcidin levels, and inflammatory markers.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for Guanosine 5'-diphosphate disodium salt are limited. The compound is soluble in water (50 mg/mL, clear, colorless) and has a molecular weight of 487.16. As a nucleotide, it is expected to be rapidly metabolized by phosphatases and nucleotidases in the circulation and tissues. It is unlikely to have significant oral bioavailability and is typically administered parenterally in research settings.
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| Toxicity/Toxicokinetics |
Toxicological data for Guanosine 5'-diphosphate disodium salt are limited. As an endogenous nucleotide, it is generally considered to have low toxicity. No significant acute toxicity has been reported. Standard laboratory safety precautions apply. The compound is designated for research use only.
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| References | |
| Additional Infomation |
Guanosine 5'-diphosphate disodium salt (GDP-Na₂) is a nucleoside diphosphate that activates ATP-sensitive K⁺ channels, blocks hepcidin-ferroportin interaction, and modulates the IL-6/STAT-3 pathway. It improves turpentine-induced inflammatory anemia in mice. It is used to study GTPase kinetics, GPCR signaling, and iron metabolism. It is not a drug and has no clinical approval.
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| Molecular Formula |
C10H13N5NA2O11P2
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|---|---|
| Molecular Weight |
487.1642
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| Exact Mass |
486.988
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| CAS # |
7415-69-2
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| Related CAS # |
Guanosine 5'-diphosphate;146-91-8;Guanosine 5'-diphosphate sodium;43139-22-6
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| PubChem CID |
135818279
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| Appearance |
White to off-white solid powder
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| Boiling Point |
961ºC at 760 mmHg
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| Flash Point |
535ºC
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
13
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
30
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| Complexity |
750
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| Defined Atom Stereocenter Count |
4
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| SMILES |
C1=NC2=C(N1[C@H]3[C@@H]([C@@H]([C@H](O3)COP(=O)([O-])OP(=O)(O)[O-])O)O)N=C(NC2=O)N.[Na+].[Na+]
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| InChi Key |
LTZCGDIGAHOTKN-LGVAUZIVSA-L
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| InChi Code |
InChI=1S/C10H15N5O11P2.2Na/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(25-9)1-24-28(22,23)26-27(19,20)21;;/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H2,19,20,21)(H3,11,13,14,18);;/q;2*+1/p-2/t3-,5-,6-,9-;;/m1../s1
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| Chemical Name |
disodium;[[(2R,3S,4R,5R)-5-(2-amino-6-oxo-1H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-oxidophosphoryl] hydrogen phosphate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~62.5 mg/mL (~128.29 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (205.27 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.0527 mL | 10.2636 mL | 20.5271 mL | |
| 5 mM | 0.4105 mL | 2.0527 mL | 4.1054 mL | |
| 10 mM | 0.2053 mL | 1.0264 mL | 2.0527 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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