| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
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| ln Vitro |
Glycitein (0-30 μM, 4 days/20 hours) suppresses the development and DNA synthesis of aortic smooth muscle cells (SMC) from stroke-prone spontaneous vessels (SHRSP) that are induced by glucose-coated charcoal/fetal bovine serum (DDC-FBS) [3]. Glycitein (100 μg/mL, 2 days) reduces Aβ-induced toxicity and induction in button nematodes Oxidation[6]. It also causes apoptosis, inhibits human cell viability, and promotes the G0/G1 phase.
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| ln Vivo |
Daidzin (3 mg/day, oral gavage, 4 days) will raise the embryonic weight of weaned female B6D2F1 mice, despite its limited effect on female growth [1]. Soybean supplementation (15, 30, or 45 mg/kg) can improve the antioxidant index, lower the amount of MDA in sow yarn and milk, improve the composition of milk, and boost piglet growth in sows throughout the late pregnancy and fetal phase. performance [5].
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| Cell Assay |
Cell viability assay [4]
Cell Types: human gastric cancer cells (AGS, MKN-28, 4]. MKN-45, NCI-N87, YCC-1, YCC-6, SNU-5, SNU-216, SNU-484, SNU-668) Tested Concentrations: 24 hrs (hours) Incubation Duration: 0-100 μM Experimental Results: IC50: 30.98, 60.17 are 35.07, 36.05, 33.11, 88.62, 97.68, 83.02, 46.87, 87.55 μM respectively. Apoptosis analysis [4] Cell Types: AGS Cell Tested Concentrations: 30 μM Incubation Duration: 3, 6, 12 and 24 hrs (hours) Experimental Results: Increased expression of Bax, Caspase-3 and cleaved PARP proteins and diminished Bcl-2 levels. Increase the fluorescence intensity of PI staining. |
| ADME/Pharmacokinetics |
Metabolism / Metabolites
Glycitein has known human metabolites that include (2S,3S,4S,5R)-3,4,5-trihydroxy-6-[3-(4-hydroxyphenyl)-6-methoxy-4-oxochromen-7-yl]oxyoxane-2-carboxylic acid. |
| References | |
| Additional Infomation |
Glycitein is a methoxyisoflavone that is isoflavone substituted by a methoxy group at position 6 and hydroxy groups at positions 7 and 4'. It has been isolated from the mycelia of the fungus Cordyceps sinensis. It has a role as a plant metabolite, a phytoestrogen and a fungal metabolite. It is a methoxyisoflavone and a 7-hydroxyisoflavone. It is functionally related to an isoflavone.
Glycitein has been reported in Salvia hispanica, Glycine max, and other organisms with data available. |
| Molecular Formula |
C16H12O5
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|---|---|
| Molecular Weight |
284.26
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| Exact Mass |
284.068
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| CAS # |
40957-83-3
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| PubChem CID |
5317750
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| Appearance |
White to off-white solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
547.4±50.0 °C at 760 mmHg
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| Melting Point |
>300ºC
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| Flash Point |
210.1±23.6 °C
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| Vapour Pressure |
0.0±1.5 mmHg at 25°C
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| Index of Refraction |
1.669
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| LogP |
2.57
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
21
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| Complexity |
424
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
DXYUAIFZCFRPTH-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C16H12O5/c1-20-15-6-11-14(7-13(15)18)21-8-12(16(11)19)9-2-4-10(17)5-3-9/h2-8,17-18H,1H3
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| Chemical Name |
7-hydroxy-3-(4-hydroxyphenyl)-6-methoxychromen-4-one
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| Synonyms |
Q100465; Q-100465; Glycitein
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~25 mg/mL (~87.95 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (8.79 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.79 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.5179 mL | 17.5895 mL | 35.1791 mL | |
| 5 mM | 0.7036 mL | 3.5179 mL | 7.0358 mL | |
| 10 mM | 0.3518 mL | 1.7590 mL | 3.5179 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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