| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg | |||
| 100mg | |||
| Other Sizes |
| Targets |
DENV type 2 (DENV2) E protein (envelope protein). Glabranine interacts with the soluble ectodomain of DENV2 E protein.
|
|---|---|
| ln Vitro |
Glabranine exerts an inhibitory activity against dengue virus in vitro. It interacts with the soluble ectodomain of DENV type 2 (DENV2) E protein. No specific IC50 or EC50 values are reported.
|
| ln Vivo |
No specific in vivo activity data is available for Glabranine. Its in vitro inhibitory activity against dengue virus suggests potential for in vivo studies in dengue virus infection models.
|
| Enzyme Assay |
In vitro assays for Glabranine involve measuring its antiviral activity against dengue virus. Viral replication is assessed in infected cell cultures treated with the compound. The interaction of Glabranine with the DENV2 E protein can be studied using surface plasmon resonance (SPR) or other biophysical methods.
|
| Cell Assay |
The in vitro cellular activity of Glabranine is evaluated in cell lines infected with dengue virus. Cells are treated with the compound, and viral replication is measured by plaque assay, qRT-PCR, or immunofluorescence. Cytotoxicity is assessed to differentiate antiviral effects from general toxicity.
|
| Animal Protocol |
No specific in vivo animal experimental protocols are available for Glabranine. For studying its anti-dengue activity in vivo, the compound could be administered to mouse models of dengue virus infection. Endpoints would include viral load, clinical signs, and survival.
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| ADME/Pharmacokinetics |
Glabranine has a molecular formula of C20H20O4 and a molecular weight of 324.37. It is a natural flavonoid extracted from Tephrosia sp. IUPAC name: (2S)-5,7-dihydroxy-8-(3-methylbut-2-en-1-yl)-2-phenyl-3,4-dihydro-2H-1-benzopyran-4-one.
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| Toxicity/Toxicokinetics |
No specific toxicological data is available for Glabranine. As a natural flavonoid, it is expected to have relatively low toxicity, though this has not been formally evaluated. Standard laboratory safety precautions should be observed.
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| References | |
| Additional Infomation |
Glabranin is a dihydroxyflavanone formed by the substitution of pinocembrin at the 8-position with an isopentenyl group. It is a plant metabolite. It is a dihydroxyflavanone, and also a (2S)-flavan-4-one. Functionally, it is related to pinocembrin. Glabranin has been reported to be found in Houttuynia cordata (Piscidia piscipula), licorice (Glycyrrhiza uralensis), and several other organisms with relevant data.
Glabranine is a natural flavonoid from Tephrosia sp that exerts inhibitory activity against dengue virus in vitro by interacting with the DENV2 E protein. It is not approved for clinical use and is intended for research purposes only. |
| Molecular Formula |
C20H20O4
|
|---|---|
| Molecular Weight |
324.3704
|
| Exact Mass |
324.136
|
| CAS # |
41983-91-9
|
| PubChem CID |
124049
|
| Appearance |
White to off-white solid powder
|
| Density |
1.2±0.1 g/cm3
|
| Boiling Point |
545.9±50.0 °C at 760 mmHg
|
| Flash Point |
197.1±23.6 °C
|
| Vapour Pressure |
0.0±1.5 mmHg at 25°C
|
| Index of Refraction |
1.622
|
| LogP |
6.02
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
24
|
| Complexity |
476
|
| Defined Atom Stereocenter Count |
1
|
| SMILES |
CC(=CCC1=C2C(=C(C=C1O)O)C(=O)C[C@H](O2)C3=CC=CC=C3)C
|
| InChi Key |
DAWSYIQAGQMLFS-SFHVURJKSA-N
|
| InChi Code |
InChI=1S/C20H20O4/c1-12(2)8-9-14-15(21)10-16(22)19-17(23)11-18(24-20(14)19)13-6-4-3-5-7-13/h3-8,10,18,21-22H,9,11H2,1-2H3/t18-/m0/s1
|
| Chemical Name |
(2S)-5,7-dihydroxy-8-(3-methylbut-2-enyl)-2-phenyl-2,3-dihydrochromen-4-one
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~308.29 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (7.71 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (7.71 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0829 mL | 15.4145 mL | 30.8290 mL | |
| 5 mM | 0.6166 mL | 3.0829 mL | 6.1658 mL | |
| 10 mM | 0.3083 mL | 1.5414 mL | 3.0829 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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