| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| ln Vitro |
The anti-inflammatory effects of α-terminal acid (ALA) are enhanced by ginsenoside Rb2, and this enhancement is fully reliant on GPR120 activation. On lipopolysaccharide (LPS)-stimulated macrophage RAW264.7 cells, ginsenoside Rb2 exhibits anti-inflammatory properties through upregulating GPR120 expression, which in turn amplifies ω-3-induced GPR120 activation. Ginsenoside Rb2 decreases cholesterol and triacylglycerol levels in 3T3-L1 cells by lowering oxidative damage and enhances circulation in liver cells by activating AMPK. The anti-UV properties of ginsenoside Rb2 are seen in mouse bone marrow mesenchymal stem cells (BMMSCs). The results of the MTT assay demonstrated that ginsenoside Rb2 (up to 100 μM) had no discernible effect on RAW264.7 cells, whether ALA was present or not. Ginsenoside Rb2 (0.1-100 μM; 12 h) was dependent on GPR120 expression being upregulated. GPR120 mRNA expression increased by 2.8 times when RAW264.7 macrophages were incubated with Ginsenoside Rb2 (10 μM) for a brief duration of 12 hours, according to real-time PCR data. Furthermore, ginsenoside Rb2 induced a time-dependent rise in GPR120 expression that began as early as 6 hours. According to these findings, Rb2 increases GPR120 expression in RAW264.7 macrophages in a time- and dose-dependent way [1].
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| ln Vivo |
Rotavirus (RV) infection can be avoided with the antiviral drug ginsenoside Rb2. This ginsenoside treatment proved most successful when administered at a dose of 75 mg/kg three days before to viral challenge, even when varying doses of ginsenoside Rb2 (25 to 250 mg/kg) were provided three, two, or one day prior to viral challenge. minimizes diarrhea caused by the rotavirus (RV). Furthermore, it was found that administering ginsenoside Rb2 (75 mg/kg) continuously for 3, 2, and 1 days prior to viral infection was more effective than administering it only once on day 3. RV infections are destroyed when ginsenoside Rb2 is continuously formulated because it lowers viral titers [2].
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| References |
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| Additional Infomation |
Ginsenoside Rb2 is a ginsenoside found in Panax ginseng that is dammarane which is substituted by hydroxy groups at the 3beta, 12beta and 20 pro-S positions, in which the hydroxy groups at positions 3 and 20 have been converted to the corresponding beta-D-glucopyranosyl-(1->2)-beta-D-glucopyranoside and alpha-L-arabinopyranosyl-(1->6)-beta-D-glucopyranoside respectively, and in which a double bond has been introduced at the 24-25 position. It has a role as a plant metabolite, an antiviral agent and a hypoglycemic agent. It is a 12beta-hydroxy steroid, a beta-D-glucoside, a disaccharide derivative, a ginsenoside and a tetracyclic triterpenoid. It derives from a hydride of a dammarane.
Ginsenoside Rb2 has been reported in Gynostemma yixingense, Panax notoginseng, and other organisms with data available. See also: Asian Ginseng (part of); Panax notoginseng root (part of). |
| Molecular Formula |
C53H90O22
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|---|---|
| Molecular Weight |
1079.28
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| Exact Mass |
1078.592
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| CAS # |
11021-13-9
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| PubChem CID |
6917976
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| Appearance |
White to off-white solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
1117.1±65.0 °C at 760 mmHg
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| Melting Point |
197-199ºC
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| Flash Point |
629.4±34.3 °C
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| Vapour Pressure |
0.0±0.6 mmHg at 25°C
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| Index of Refraction |
1.622
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| LogP |
4.73
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| Hydrogen Bond Donor Count |
14
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| Hydrogen Bond Acceptor Count |
22
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| Rotatable Bond Count |
15
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| Heavy Atom Count |
75
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| Complexity |
1950
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| Defined Atom Stereocenter Count |
29
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| SMILES |
CC(=CCC[C@@](C)([C@H]1CC[C@@]2([C@@H]1[C@@H](C[C@H]3[C@]2(CC[C@@H]4[C@@]3(CC[C@@H](C4(C)C)O[C@H]5[C@@H]([C@H]([C@@H]([C@H](O5)CO)O)O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O)C)C)O)C)O[C@H]7[C@@H]([C@H]([C@@H]([C@H](O7)CO[C@H]8[C@@H]([C@H]([C@H](CO8)O)O)O)O)O)O)C
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| InChi Key |
NODILNFGTFIURN-GZPRDHCNSA-N
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| InChi Code |
InChI=1S/C53H90O22/c1-23(2)10-9-14-53(8,75-47-43(67)39(63)37(61)29(72-47)22-69-45-41(65)34(58)26(57)21-68-45)24-11-16-52(7)33(24)25(56)18-31-50(5)15-13-32(49(3,4)30(50)12-17-51(31,52)6)73-48-44(40(64)36(60)28(20-55)71-48)74-46-42(66)38(62)35(59)27(19-54)70-46/h10,24-48,54-67H,9,11-22H2,1-8H3/t24-,25+,26-,27+,28+,29+,30-,31+,32-,33-,34-,35+,36+,37+,38-,39-,40-,41+,42+,43+,44+,45-,46-,47-,48-,50-,51+,52+,53-/m0/s1
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| Chemical Name |
(2S,3R,4S,5S,6R)-2-[(2R,3R,4S,5S,6R)-4,5-dihydroxy-6-(hydroxymethyl)-2-[[(3S,5R,8R,9R,10R,12R,13R,14R,17S)-12-hydroxy-4,4,8,10,14-pentamethyl-17-[(2S)-6-methyl-2-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-[[(2S,3R,4S,5S)-3,4,5-trihydroxyoxan-2-yl]oxymethyl]oxan-2-yl]oxyhept-5-en-2-yl]-2,3,5,6,7,9,11,12,13,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-3-yl]oxy]oxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol
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| Synonyms |
NSC 308878; Ginsenoside RB2; Ginsenoside C
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ≥ 100 mg/mL (~92.66 mM)
DMSO : ~9.5 mg/mL (~8.80 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 0.92 mg/mL (0.85 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 9.2 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 0.92 mg/mL (0.85 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 9.2 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 0.92 mg/mL (0.85 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 100 mg/mL (92.66 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.9265 mL | 4.6327 mL | 9.2654 mL | |
| 5 mM | 0.1853 mL | 0.9265 mL | 1.8531 mL | |
| 10 mM | 0.0927 mL | 0.4633 mL | 0.9265 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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