| Size | Price | Stock | Qty |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg | |||
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| Targets |
Ginsenoside Rb1 targets multiple pathways and molecules. It inhibits Na+, K+-ATPase activity. It modulates the phosphorylation of synapsins through a cAMP-dependent protein kinase pathway, promoting neurotransmitter release. It also regulates the pregnane X receptor (PXR)/NF-κB signaling. Its effects on wound healing are mediated through these pathways.
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| ln Vitro |
Ginsenoside Rb1's IC50 against Na+ and K+-ATPase is 6.3±1.0 μM. Simultaneously, ginsenoside is a non-competitive ATP coupling, as demonstrated by the kinetic analysis of the rise in ginsenoside Rb1 concentration or the increase in Na+ and K+ concentration [1]. The reduction of IKK-β and IRAK-1 receptor-associated pump 1 (IRAK-1) was significantly inhibited by ginsenoside Rb1, which also improved the inhibitory effect. MAP moieties (ERK, JNK, and p-38) and NF-κB activation; however, no connection has been found between LPS and Toll-like receptor 4 responses, IRAK-2 activation, or IRAK-4 activation [2]. Rb1 is a part of ginseng, a traditional Chinese medicine. The primary bioactive ingredient that controls pro-alkane X receptor (PXR)/NF-κB signaling is ginsenoside Rb1. Ginsenoside Rb1 in ginsenoside extract (GSE) exhibits strong anti-inflammatory properties. Based on preliminary studies, the concentration of ginsenoside Rb1 (10 μM) in cells was optimized to ensure adequate anti-inflammatory activity without evident toxicity. Ginsenoside Rb1 returned the mRNA levels of PXR and CYP3A4 to LS174T cells and significantly decreased the TNF-α-induced upregulation of IL-1β and iNOS. Ginsenoside Rb1 significantly eliminated both the significant increase in the ratio of phosphorylated to total NF-κB p65 and the significant decrease in PXR protein levels caused by TNF-α [3].
In vitro, Ginsenoside Rb1 inhibits Na+, K+-ATPase activity with an IC50 of 6.3 μM. It promotes neurotransmitter release by modulating synapsin phosphorylation via a cAMP-dependent protein kinase pathway. It has shown antiproliferative effects against various cancer cell lines. It also has immunostimulatory and anti-oxidative activities. |
| ln Vivo |
Ginsenoside Rb1, at doses of 30 mg/kg and 60 mg/kg, significantly reduced lung damage as shown by histology. In older adults, ginsenoside Rb1 at 30 mg/kg and 60 mg/kg significantly reduced histological lung damage [4]. Rb1) has a positive impact on the underlying mechanism of intestinal membrane microvascular wafer permeability generated by lipopolysaccharide (LPS) and is a constituent of the traditional Chinese medication ginseng. Thirty minutes after cosmetic LPS infusion, ginsenoside Rb1 (5 mg/kg) flowed via the left jugular system in certain stents. Within microvessels, ginsenoside Rb1 decreases the amount of caveolae in the cell skin. Due to its capacity to suppress NF-κB and Src activation, ginsenoside Rb1 improves microvascular hyperpermeability following endotoxin demand and eliminates intestinal edema by decreasing development of caveolae and connections [5].
Ginsenoside Rb1 exhibits strong burn wound-healing action in vivo at low concentrations (100 pg/g to 10 fg/g ointment). It has anti-oxidative, immunostimulatory, and anticancer activities in animal models. Its effects on the PXR/NF-κB signaling pathway have also been studied in vivo. |
| Enzyme Assay |
In vitro enzyme/receptor binding assays for Ginsenoside Rb1 involve measuring its inhibition of Na+, K+-ATPase activity. These assays typically use purified enzyme preparations and appropriate substrates to determine the IC50 value. Its binding to the pregnane X receptor (PXR) has also been studied.
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| Cell Assay |
In vitro cell-based assays for Ginsenoside Rb1 involve treating various cell lines to assess its effects on cell proliferation, neurotransmitter release, and signaling pathways. Its effects on synapsin phosphorylation are studied in neuronal cells. Its immunostimulatory and anti-oxidative activities are assessed in immune cells and other cell types.
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| Animal Protocol |
In vivo animal experiments for Ginsenoside Rb1 have been conducted in models of burn wound healing. The compound is typically applied topically as an ointment, and its effects on wound closure and tissue regeneration are assessed. Its anticancer and immunostimulatory effects have also been studied in various animal models.
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| ADME/Pharmacokinetics |
Ginsenoside Rb1 is a major component of Panax ginseng. Its pharmacokinetic properties, including absorption, distribution, metabolism, and excretion, have been studied in the context of ginseng consumption. It is a large glycosylated molecule, which may influence its oral bioavailability.
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| Toxicity/Toxicokinetics |
Specific toxicity data for Ginsenoside Rb1 is not provided in the search results. As a major component of a widely consumed herbal medicine, it is generally considered to have a favorable safety profile. However, high doses may have adverse effects, and its safety in specific populations has not been fully established.
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| References |
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| Additional Infomation |
Ginsenoside Rb1, found in ginseng (Panax ginseng) and Japanese ginseng (Panax japonicus var. major), is a derivative of ginsenoside Rd, in which the β-D-glucopyranoside group at position 20 is replaced by a β-D-glucopyranoside-β-D-glucopyranoside group. It possesses multiple functions, including neuroprotection, anti-obesity, anti-inflammation, inhibition of apoptosis, free radical scavenging, and as a plant metabolite. It is a ginsenoside, glycoside, and tetracyclic triterpenoid compound functionally related to ginsenoside Rd. Ginsenosides are a class of steroidal glycosides and triterpenoid saponins, found only in plants of the genus Panax. Ginsenosides have been a focus of research because they are considered the active ingredients behind ginseng's efficacy. Their effects are complex and difficult to isolate because ginsenosides appear to affect multiple pathways. Rb1 is most abundant in American ginseng (Panax quinquefolius). Rb1 appears to affect the reproductive system of the testes in animals. Recent studies have shown that Rb1 affects rat embryonic development and has teratogenic effects, leading to birth defects. Another study suggests that Rb1 may indirectly increase testosterone production in male rats by stimulating luteinizing hormone. Ginsenoside rb1 has been reported to be present in Japanese ginseng (Panax japonicus), licorice (Glycyrrhiza uralensis), and several other organisms with relevant data. See also: American ginseng (partial); Asian ginseng (partial); Panax notoginseng root (partial).
Ginsenoside Rb1 is a major bioactive ginsenoside from Panax ginseng with diverse pharmacological activities, including wound healing, neuroprotective, and anticancer effects. It is one of the most abundant ginsenosides in ginseng and has been extensively studied for its health benefits. |
| Molecular Formula |
C54H92O23
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|---|---|
| Molecular Weight |
1109.2945
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| Exact Mass |
1108.602
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| CAS # |
41753-43-9
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| PubChem CID |
9898279
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| Appearance |
White to off-white solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
1145.9±65.0 °C at 760 mmHg
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| Flash Point |
646.8±34.3 °C
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| Vapour Pressure |
0.0±0.6 mmHg at 25°C
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| Index of Refraction |
1.626
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| LogP |
2.9
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| Hydrogen Bond Donor Count |
15
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| Hydrogen Bond Acceptor Count |
23
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| Rotatable Bond Count |
16
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| Heavy Atom Count |
77
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| Complexity |
2000
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| Defined Atom Stereocenter Count |
30
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| SMILES |
CC(=CCC[C@@](C)([C@H]1CC[C@@]2([C@@H]1[C@@H](C[C@H]3[C@]2(CC[C@@H]4[C@@]3(CC[C@@H](C4(C)C)O[C@H]5[C@@H]([C@H]([C@@H]([C@H](O5)CO)O)O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O)C)C)O)C)O[C@H]7[C@@H]([C@H]([C@@H]([C@H](O7)CO[C@H]8[C@@H]([C@H]([C@@H]([C@H](O8)CO)O)O)O)O)O)O)C
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| InChi Key |
GZYPWOGIYAIIPV-JBDTYSNRSA-N
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| InChi Code |
InChI=1S/C54H92O23/c1-23(2)10-9-14-54(8,77-48-44(69)40(65)37(62)29(74-48)22-70-46-42(67)38(63)34(59)26(19-55)71-46)24-11-16-53(7)33(24)25(58)18-31-51(5)15-13-32(50(3,4)30(51)12-17-52(31,53)6)75-49-45(41(66)36(61)28(21-57)73-49)76-47-43(68)39(64)35(60)27(20-56)72-47/h10,24-49,55-69H,9,11-22H2,1-8H3/t24-,25+,26+,27+,28+,29+,30-,31+,32-,33-,34+,35+,36+,37+,38-,39-,40-,41-,42+,43+,44+,45+,46+,47-,48-,49-,51-,52+,53+,54-/m0/s1
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| Chemical Name |
(2R,3R,4S,5S,6R)-2-[[(2R,3S,4S,5R,6S)-6-[(2S)-2-[(3S,5R,8R,9R,10R,12R,13R,14R,17S)-3-[(2R,3R,4S,5S,6R)-4,5-dihydroxy-6-(hydroxymethyl)-3-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]oxy-12-hydroxy-4,4,8,10,14-pentamethyl-2,3,5,6,7,9,11,12,13,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-17-yl]-6-methylhept-5-en-2-yl]oxy-3,4,5-trihydroxyoxan-2-yl]methoxy]-6-(hydroxymethyl)oxane-3,4,5-triol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~90.15 mM)
H2O : ≥ 33.33 mg/mL (~30.05 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (2.25 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (2.25 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (2.25 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.9015 mL | 4.5073 mL | 9.0147 mL | |
| 5 mM | 0.1803 mL | 0.9015 mL | 1.8029 mL | |
| 10 mM | 0.0901 mL | 0.4507 mL | 0.9015 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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