| Size | Price | Stock | Qty |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
Gibberellin A4 primarily targets gibberellin receptors in plants. In the context of biomedical research, its key molecular target of interest is the transcription factor NF-κB, where it exhibits anti-NF-κB activity. By modulating this pathway, Gibberellin A4 can reduce inflammation, as demonstrated by its ability to decrease LPS-induced NF-κB activation in RAW 264.7 macrophages.
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| ln Vitro |
In vitro, Gibberellin A4 demonstrates significant anti-NF-κB and antioxidant activities. It has been shown to reduce LPS-induced NF-κB activation in RAW 264.7 macrophages, highlighting its potential as an anti-inflammatory agent. These activities make it a useful compound for investigating inflammatory pathways in cell-based systems, where it can modulate key signaling cascades involved in the immune response.
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| ln Vivo |
In vivo, Gibberellin A4 is primarily recognized for its potent plant growth-regulating effects, promoting flowering and other developmental processes. While its anti-inflammatory and antioxidant properties have been characterized in vitro, specific in vivo efficacy data in animal models of disease are not detailed in the available sources. Its documented anti-NF-κB activity suggests potential for in vivo applications in inflammation research.
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| Enzyme Assay |
In vitro assays for Gibberellin A4's anti-inflammatory activity typically involve culturing macrophage cell lines, such as RAW 264.7 cells, and stimulating them with lipopolysaccharide (LPS) to induce an inflammatory response. The cells are then treated with Gibberellin A4, and the inhibition of NF-κB activation is measured using reporter gene assays or by quantifying the expression of NF-κB target genes. Antioxidant activity can be assessed using standard cell-based ROS detection assays.
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| Cell Assay |
Cellular assays for Gibberellin A4 are conducted using mammalian cell lines like RAW 264.7 macrophages. The general protocol involves seeding cells, treating them with varying concentrations of Gibberellin A4, and then stimulating with LPS. After incubation, cells are harvested, and the levels of NF-κB activation are measured through methods such as Western blotting for phosphorylated IκB or by using NF-κB luciferase reporter constructs.
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| Animal Protocol |
In vivo studies for Gibberellin A4 in animal models are not detailed in the available research literature. As a plant hormone, its primary in vivo applications are in botanical research. For biomedical applications, the compound's documented anti-inflammatory mechanism suggests that it could be evaluated in standard animal models of inflammation, such as the carrageenan-induced paw edema model or models of periodontitis, but such studies have not been reported in the provided sources.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for Gibberellin A4 are not available in the provided sources. The compound has a molecular weight of 332.39 g/mol and is typically stored as a powder at -20°C for up to 3 years. It is soluble in DMSO. For research purposes, it can be dissolved in appropriate solvents for in vitro assays. No specific information regarding its absorption, distribution, metabolism, or excretion is provided.
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| Toxicity/Toxicokinetics |
Toxicity data for Gibberellin A4 are not reported in the available sources. As a naturally occurring plant hormone, it is generally considered to have a favorable safety profile. The compound is intended for research use only and is not approved for human therapeutic use. Specific toxicological studies have not been described in the provided literature.
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| References | |
| Additional Infomation |
Gibberellin A4 is a C19-gibberellin, initially discovered in Gibberella fujikuroi, differing from gibberellin A1 in that the hydroxyl group at the C-7 position (gibberellin number) is replaced by a hydrogen atom. It is a plant metabolite. It is a lactone, belonging to the gibberellin monocarboxylic acid family, and is also a C19-gibberellin. It is the conjugate acid of gibberellin A4(1-). Gibberellin A4 has been reported to exist in Fusarium fujikuroi, citrus (Citrus reticulata), and several other organisms with relevant data.
Gibberellin A4 is a plant hormone with emerging biomedical research applications due to its anti-NF-κB and antioxidant activities. It is a useful tool for studying inflammatory pathways. The compound is available from commercial suppliers for research purposes only. It is not an approved therapeutic agent. Its mechanism of action, involving the modulation of NF-κB signaling, makes it a compound of interest for investigating inflammation-related diseases. |
| Molecular Formula |
C19H24O5
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|---|---|
| Molecular Weight |
332.39
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| Exact Mass |
332.162
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| CAS # |
468-44-0
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| Related CAS # |
Gibberellin A4-13C,d3;Gibberellin A4-d2
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| PubChem CID |
92109
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| Appearance |
White to off-white solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
574.2±50.0 °C at 760 mmHg
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| Melting Point |
201 - 205ºC
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| Flash Point |
210.4±23.6 °C
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| Vapour Pressure |
0.0±3.6 mmHg at 25°C
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| Index of Refraction |
1.615
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| LogP |
1.7
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
24
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| Complexity |
685
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| Defined Atom Stereocenter Count |
8
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| SMILES |
C[C@@]12[C@H](CC[C@@]3([C@@H]1[C@@H]([C@]45[C@H]3CC[C@H](C4)C(=C)C5)C(=O)O)OC2=O)O
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| InChi Key |
RSQSQJNRHICNNH-NFMPGMCNSA-N
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| InChi Code |
InChI=1S/C19H24O5/c1-9-7-18-8-10(9)3-4-11(18)19-6-5-12(20)17(2,16(23)24-19)14(19)13(18)15(21)22/h10-14,20H,1,3-8H2,2H3,(H,21,22)/t10-,11-,12+,13-,14-,17-,18+,19-/m1/s1
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| Chemical Name |
(1R,2R,5R,8R,9S,10R,11S,12S)-12-hydroxy-11-methyl-6-methylidene-16-oxo-15-oxapentacyclo[9.3.2.15,8.01,10.02,8]heptadecane-9-carboxylic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~300.85 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (7.52 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (7.52 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (7.52 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0085 mL | 15.0426 mL | 30.0851 mL | |
| 5 mM | 0.6017 mL | 3.0085 mL | 6.0170 mL | |
| 10 mM | 0.3009 mL | 1.5043 mL | 3.0085 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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