| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| 5mg | |||
| 100mg | |||
| Other Sizes |
| Targets |
Others (not specifically classified). Gentianine has anti-diabetic, antipsychotic, and anti-inflammatory activities. Its anti-inflammatory action might be at least partly based on the suppressed production of TNF-alpha and IL-6.
|
|---|---|
| ln Vitro |
Gentianine has anti-diabetic effect, antipsychotic activity, and potential anti-inflammatory action. Its anti-inflammatory action might be at least partly based on the suppressed production of TNF-alpha and IL-6. No specific IC50 or EC50 values are reported.
|
| ln Vivo |
No specific in vivo activity data is available for Gentianine. Its anti-diabetic, antipsychotic, and anti-inflammatory activities suggest potential for in vivo studies in diabetes, psychiatric, and inflammatory disease models.
|
| Enzyme Assay |
In vitro assays for Gentianine typically involve measuring its anti-inflammatory activity by assessing the suppression of TNF-alpha and IL-6 production in activated immune cells (e.g., macrophages). Anti-diabetic activity can be assessed by measuring glucose uptake or insulin secretion in cell-based assays.
|
| Cell Assay |
The in vitro cellular activity of Gentianine is evaluated in immune cells (e.g., macrophages, microglia) for its anti-inflammatory effects. Cells are stimulated with LPS or other inflammatory stimuli, and cytokine production (TNF-alpha, IL-6) is measured by ELISA or qRT-PCR. Anti-diabetic effects can be assessed in insulin-sensitive cell lines.
|
| Animal Protocol |
No specific in vivo animal experimental protocols are available for Gentianine. For studying its anti-inflammatory effects, the compound could be administered to rodent models of inflammation (e.g., LPS-induced sepsis, carrageenan-induced paw edema). For anti-diabetic studies, models of diabetes (e.g., STZ-induced diabetes, db/db mice) could be used.
|
| ADME/Pharmacokinetics |
Gentianine has a molecular formula of C10H9NO2 and a molecular weight of 175.18. It is a pyranopyridine compound belonging to the lactone class and pyridine alkaloids. Gentianine is also known as 5-vinyl-3,4-dihydro-1H-pyrano[3,4-c]pyridin-1-one.
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| Toxicity/Toxicokinetics |
No specific toxicological data is available for Gentianine. As a natural alkaloid, it may have unknown toxicity. Standard laboratory safety precautions should be observed.
|
| References |
|
| Additional Infomation |
Gentianine is a pyranopyridine compound, belonging to the lactone class and pyridine alkaloids. It has been reported to exist in Hunteria zeylanica, Swertia ciliata, and other organisms with relevant data. See also: fenugreek seeds (partial); whole plant of Centaurium erythraea (partial).
Gentianine is a pyranopyridine alkaloid with anti-diabetic, antipsychotic, and anti-inflammatory activities. Its anti-inflammatory action might be based on the suppressed production of TNF-alpha and IL-6. It is not approved for clinical use and is intended for research purposes only. |
| Molecular Formula |
C10H9NO2
|
|---|---|
| Molecular Weight |
175.1840
|
| Exact Mass |
175.063
|
| CAS # |
439-89-4
|
| PubChem CID |
354616
|
| Appearance |
White to off-white solid powder
|
| Density |
1.2±0.1 g/cm3
|
| Boiling Point |
386.5±42.0 °C at 760 mmHg
|
| Melting Point |
84-86ºC
|
| Flash Point |
187.6±27.9 °C
|
| Vapour Pressure |
0.0±0.9 mmHg at 25°C
|
| Index of Refraction |
1.604
|
| Source |
Originated from plants: Gentianaceae Gentiana macrophylla Pall.
|
| LogP |
1.73
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
1
|
| Heavy Atom Count |
13
|
| Complexity |
227
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
O=C1OCCC2=C1C=NC=C2C=C
|
| InChi Key |
DFNZYFAJQPLJFI-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C10H9NO2/c1-2-7-5-11-6-9-8(7)3-4-13-10(9)12/h2,5-6H,1,3-4H2
|
| Chemical Name |
5-ethenyl-3,4-dihydropyrano[3,4-c]pyridin-1-one
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: ~50 mg/mL (~285.4 mM; with ultrasonication)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (7.14 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (12.5 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (7.14 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (12.5 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.7084 mL | 28.5421 mL | 57.0841 mL | |
| 5 mM | 1.1417 mL | 5.7084 mL | 11.4168 mL | |
| 10 mM | 0.5708 mL | 2.8542 mL | 5.7084 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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