| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| Other Sizes |
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| ln Vitro |
Gap 26 (0.25 mg/mL, 30 min) reduced channel size in cell lines (RBE4, SV-ARBEC, and ECV304). Gap 26 (0.25 mg/mL, 30 minutes) totally reduced the InsP3-triggered ATP response, lowering ATP release below control levels, showing that basal ATP release is also affected [1]. Gap 26 did considerably limit our InsP3-triggered intercellular calcium waves, but not dye conjugation across connected channels. Gap 26 (100-300 μM) was required to diminish the rhythmic response of the rabbit superior mesenteric artery with an IC50 of 28.4 ± 3.4 μM[ any effect, as evidenced by FRAP tests, despite connexin 43 in cell lines [1]. 2].
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| References |
[1]. Katleen Braet, et al. Photoliberating inositol-1,4,5-trisphosphate triggers ATP release that is blocked by the connexin mimetic peptide gap 26. Cell Calcium. 2003 Jan;33(1):37-48.
[2]. Chaytor AT, et al. Peptides homologous to extracellular loop motifs of connexin 43 reversibly abolish rhythmic contractile activity in rabbit arteries. J Physiol. 1997 Aug 15;503 ( Pt 1):99-110. |
| Molecular Formula |
C₇₀H₁₀₆N₁₉O₁₉S
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|---|---|
| Molecular Weight |
1550.78
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| CAS # |
197250-15-0
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| Related CAS # |
Gap 26 TFA
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| SMILES |
S([H])C([H])([H])C([H])(C(N([H])C([H])(C([H])([H])C1C([H])=C([H])C(=C([H])C=1[H])O[H])C(N([H])C([H])(C([H])([H])C(=O)O[H])C(N([H])C([H])(C([H])([H])C([H])([H])C([H])([H])C([H])([H])N([H])[H])C(N([H])C([H])(C([H])([H])O[H])C(N([H])C([H])(C([H])([H])C1C([H])=C([H])C([H])=C([H])C=1[H])C(N1C([H])([H])C([H])([H])C([H])([H])C1([H])C(N([H])C([H])(C(N([H])C([H])(C(N([H])C([H])(C(N([H])C([H])(C(N([H])C([H])(C(=O)O[H])C([H])([H])C([H])([H])C([H])([H])/N=C(\N([H])[H])/N([H])[H])=O)C([H])(C([H])([H])[H])C([H])([H])[H])=O)C([H])([H])C1=C([H])N=C([H])N1[H])=O)C([H])([H])O[H])=O)C([H])(C([H])([H])[H])C([H])([H])C([H])([H])[H])=O)=O)=O)=O)=O)=O)=O)N([H])C(C([H])(C([H])(C([H])([H])[H])C([H])([H])[H])N([H])[H])=O
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| Synonyms |
Gap 26Gap-26Gap26
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~50 mg/mL (~32.24 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (64.48 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.6448 mL | 3.2242 mL | 6.4484 mL | |
| 5 mM | 0.1290 mL | 0.6448 mL | 1.2897 mL | |
| 10 mM | 0.0645 mL | 0.3224 mL | 0.6448 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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